| Serine/threonine protein phosphatase, putative [Q4Q8T8] | |
|---|---|
| Systematic Name | LmjF.26.2530 [Leishmania major] |
| Gene Name | LMJF_26_2530 |
| Molecular Weight | 55122 Da |
| Protein Sequence Size | 499 |
| Function | |
| Charge | -15 |
| Isoelectric Point | 4.8 pH |
| Description | Serine/threonine protein phosphatase, putative (EC 3.1.3.16). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 3.1.3.16 |
| Sequence | >tr|Q4Q8T8|Q4Q8T8_LEIMA Serine/threonine protein phosphatase, putative (EC 3.1.3.16) - Leishmania major LLTDRGNPSVPPPNWEPLSSSALFDESGTARPDVVREHLRKEGLLQEADALTIITQCALI WKDEPNVLRVDGPVAIAGDIHGQFFDLLNLISIGGDPSQQKYIFLGDYVDRGCFGMEVIL LLMCYKICYPDTMIMLRGNHESRHLTTYFNFKREVLYKYSIAVYNAIMSAFDCLPLACIL NNRFLCVHGGLSPELKRISDIGAIHRFREPPSSGPMCDLLWADPLDEKEEDPAAAPLFVP NTTRGCSYVYSNAAACNFLEENDLITIIRGHEAQDEGYHLYKKTSKGFPAVICIFSAPNY CDTYDNRAAVVMLNRNIMSIRQFNSSPHPYYLPNFMNAFTWSLPFVEEKLLDIVANVLHP IDGGEDHLLVDGAEAAKAAPTPGANTESRDGRVLEQRGENIREKILAMGRLSRMFHTLCE GSESSLPPKGLTGDIPPQGGLPCGPDSVQAACRGLQQSKELDSVNERWQDCLIESDLEPQ PSAATTVLSAASSSTGSR |
| DNA Sequence | >LmjF26.2530 |||serine/threonine protein phosphatase, putative|Leishmania major|chr 26|||Manual ATGCTCCTCA CAGACCGTGG CAATCCGTCA GTGCCACCGC CGAATTGGGA GCCACTGAGCAGCTCTGCGC TCTTCGACGA GAGTGGCACG GCGCGCCCCG ATGTGGTGCG CGAGCACCTCAGAAAGGAGG GTCTCCTGCA GGAGGCGGAT GCCCTGACAA TTATTACTCA GTGCGCTCTGATATGGAAAG ACGAGCCAAA CGTGCTGCGT GTCGATGGTC CGGTGGCCAT CGCTGGCGATATACACGGCC AATTTTTCGA TTTACTGAAC CTCATCTCCA TCGGCGGCGA CCCGTCCCAGCAGAAGTACA TCTTCCTGGG CGACTATGTG GACCGCGGGT GCTTCGGAAT GGAGGTGATTCTGCTACTCA TGTGCTATAA GATCTGCTAC CCCGACACGA TGATCATGCT GCGCGGCAACCACGAAAGCC GACACCTCAC GACGTACTTC AACTTCAAGC GCGAGGTGCT CTACAAGTACTCCATCGCCG TGTACAACGC CATAATGTCC GCCTTCGATT GCCTGCCGCT CGCATGCATCCTCAACAATC GATTCCTCTG CGTGCACGGC GGCTTGTCGC CGGAGCTGAA GCGGATCTCCGACATTGGTG CCATTCACCG CTTCCGCGAG CCGCCGTCAT CGGGGCCGAT GTGCGACCTGCTGTGGGCAG ACCCGCTGGA CGAAAAAGAG GAGGACCCTG CGGCGGCGCC GCTGTTCGTGCCCAACACAA CCCGCGGCTG CTCTTACGTC TACAGCAACG CTGCGGCCTG CAACTTCTTGGAGGAAAACG ACCTCATCAC AATCATCCGT GGCCACGAGG CCCAGGATGA GGGCTACCACTTGTACAAGA AGACAAGCAA GGGGTTTCCT GCGGTGATTT GCATCTTTTC CGCGCCGAACTACTGCGACA CGTACGACAA CCGGGCCGCC GTCGTCATGC TCAACCGCAA CATTATGAGCATCCGCCAGT TCAACAGCAG CCCTCACCCC TACTACCTGC CTAACTTCAT GAACGCCTTCACTTGGTCGC TGCCCTTTGT GGAGGAGAAG TTGCTCGACA TTGTGGCGAA CGTCCTCCACCCCATCGACG GTGGTGAGGA TCATCTGCTT GTGGACGGGG CGGAGGCGGC GAAGGCCGCACCGACACCGG GGGCGAACAC AGAAAGCCGC GACGGGAGAG TCCTGGAACA GCGAGGCGAGAACATTCGCG AGAAGATACT GGCGATGGGG AGGCTCTCCC GCATGTTCCA CACCTTGTGCGAAGGAAGCG AGAGCAGTCT TCCACCCAAG GGACTGACAG GTGACATACC ACCGCAGGGTGGACTGCCGT GTGGCCCCGA CAGTGTGCAA GCAGCCTGTC GCGGCCTCCA GCAATCAAAGGAGCTGGACT CCGTCAATGA GCGCTGGCAG GATTGTCTGA TCGAGAGCGA TTTGGAGCCGCAGCCGAGTG CGGCGACCAC AGTTCTCAGT GCCGCGTCCT CTAGCACCGG CAGTAGGTGA |
| Serine/threonine protein phosphatase, putative Q4Q8T8] | |
|---|---|
| Metabolite Information | |
| Molecular Function | hydrolase activity; protein serine/threonine phosphatase activity |
| Biochemical Pathway | protein amino acid dephosphorylation |
| Regulatory Pathway | |
| KEGG Pathways | K04348 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha) [Homo sapiens] | 47 | 1e-126 | 450 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YER133w serthr phosphoprotein phosphatase 1, catalytic chain | Saccharomyces cerevisiae | 35% | 1e-52 | 201 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00115 | Serine/threonine specific protein phosphatases signature | 137-142; | PS00125 | |
| Acylation | N-myristoylation site | 95-100; 139-144; 373-378; 422-427; 440-445; 455-460; | PS00008 | |
| Glycosylation | N-glycosylation site | 242-245; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 197-200; 283-286; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 170-173; 418-421; 464-467; 476-479; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 4-6; 30-32; 243-245; 285-287; 320-322; | PS00005 | |
| Serine/threonine protein phosphatase, putative [Q4Q8T8] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1auiA | |
| Percent Identity | 47% | |
| Target Region | 1-500 | |
| Template Region | 14-378 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 111 | Sidechain |
| ARG | 112 | Sidechain |
| ASN | 140 | Sidechain |
| HIS | 141 | Sidechain |
| ARG | 245 | Sidechain |
| HIS | 272 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 17787-72-3 | CALCIUM ION | 40.078 | Ca | [Ca+2] | 1aui |
| FE (III) ION | 55.845 | Fe | [Fe+3] | 1aui | |
| 23713-49-7 | ZINC ION | 65.409 | Zn | [Zn+2] | 1aui |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/738405/Q4Q8T8.pdb 2.0 499 = residues | | = | *| Ramachandran plot: 91.1% core 7.0% allow 1.4% gener 0.5% = disall | | = | +| All Ramachandrans: 11 labelled residues (out of 497) = | +| Chi1-chi2 plots: 1 labelled residues (out of 288) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 7 | *| Bond len/angle: 6.1 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.12 Covalent: -0.21 Overall: = -0.01 | | = | | M/c bond lengths: 98.3% within limits 1.7% highlighted = | | M/c bond angles: 93.9% within limits 6.1% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |