| Histone H2B variant [Q4Q8P9] | |
|---|---|
| Systematic Name | LmjF.28.0210 [Leishmania major] |
| Gene Name | LMJF_28_0210 |
| Molecular Weight | 15756 Da |
| Protein Sequence Size | 142 |
| Function | |
| Charge | 25 |
| Isoelectric Point | 11.8 pH |
| Description | Histone H2B variant. |
| Subcellular Location | Nuclear[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4Q8P9|Q4Q8P9_LEIMA Histone H2B variant - Leishmania major PPTKGGKRPIPLGGKGKGKRSAGKAPKSGNDHKRRHSHKRSQHWDLYIHRALRRFFKRGT LSKAAVRVLSSFIEDMFNRIQTEAVFVAKINKVKTLTAREIQTSARLLLPPELAKHAMSE GTKAVAKYNASRGEAYAQGGI |
| DNA Sequence | >LmjF28.0210 |||histone H2B variant|Leishmania major|chr 28|||Manual ATGCCTCCGA CCAAGGGTGG AAAACGTCCG ATCCCGCTAG GGGGTAAGGG CAAGGGCAAGCGCTCGGCGG GTAAGGCACC CAAGTCGGGC AACGATCACA AGCGCCGCCA CAGTCACAAACGCAGTCAGC ACTGGGACCT GTACATCCAT CGTGCTCTCC GCCGCTTCTT TAAGCGCGGCACGCTGAGCA AGGCGGCCGT GCGTGTGCTT TCCTCCTTTA TTGAGGACAT GTTCAACCGCATCCAGACCG AGGCCGTCTT TGTCGCCAAG ATCAACAAGG TCAAGACTTT GACTGCTCGCGAAATCCAGA CGTCGGCCCG TCTGCTGCTG CCACCGGAGC TGGCGAAGCA CGCCATGAGCGAGGGCACCA AGGCTGTCGC CAAGTACAAC GCTTCCCGCG GTGAGGCGTA TGCTCAGGGCGGCATTTAG |
| Histone H2B variant Q4Q8P9] | |
|---|---|
| Metabolite Information | |
| Molecular Function | DNA binding |
| Biochemical Pathway | DNA replication; chromosome organization and biogenesis (sensu Eukaryota); nucleosome assembly |
| Regulatory Pathway | |
| KEGG Pathways | |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | histone H2B [Homo sapiens] | 46 | 4e-19 | 90 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YHR074w weak similarity to B.subtilis spore outgrowth factor B | Saccharomyces cerevisiae | 69% | 2.9 | 25 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00308 | Histone H2B signature | 100-122; | PS00357 | |
| PDOC00015 | The rule PS00015 has been deleted from the PROSITE database and replaced by the following motif: PS50079 (Bipartite nuclear localization signal profile | 20-36; | PS00015 | |
| Amidation | Amidation site | 6-9; 18-21; | PS00009 | |
| Glycosylation | N-glycosylation site | 130-133; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 35-38; 58-61; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 29-32; 72-75; 98-101; 132-135; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 38-40; 98-100; 105-107; | PS00005 | |
| Histone H2B variant [Q4Q8P9] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2pyoH | |
| Percent Identity | 48% | |
| Target Region | 39-133 | |
| Template Region | 28-95 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 16887-00-6 | CHLORIDE ION | 35.453 | Cl | [Cl-] | 2pyo |
| 16397-91-4 | MANGANESE (II) ION | 54.938 | Mn | [Mn+2] | 2pyo |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1876290/Q4Q8P9.pdb 2.0 95 = residues | | = | | Ramachandran plot: 97.8% core 2.2% allow 0.0% gener 0.0% = disall | | = | | All Ramachandrans: 0 labelled residues (out of 93) = | +| Chi1-chi2 plots: 1 labelled residues (out of 60) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | | Residue properties: Max.deviation: 2.1 Bad contacts: = 1 | +| Bond len/angle: 3.4 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.25 Covalent: -0.08 Overall: = 0.12 | | = | | M/c bond lengths: 99.8% within limits 0.2% highlighted = | | M/c bond angles: 95.0% within limits 5.0% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |