| Propionyl-coa carboxylase beta chain, putative [Q4Q8M1] | |
|---|---|
| Systematic Name | LmjF.28.0490 [Leishmania major] |
| Gene Name | LMJF_28_0490 |
| Molecular Weight | 56723 Da |
| Protein Sequence Size | 522 |
| Function | |
| Charge | 12.5 |
| Isoelectric Point | 8.7283 pH |
| Description | Propionyl-coa carboxylase beta chain, putative (EC 6.4.1.3). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 6.4.1.3 |
| Sequence | >tr|Q4Q8M1|Q4Q8M1_LEIMA Propionyl-coa carboxylase beta chain, putative (EC 6.4.1.3) - Leishmania major. RRLGCGLVSAAMGVGGMSSSQLTKEFDRHTHTPTAAEESRHKKKRLTAMERVQLFCDPGT FRERDALVEHGCHNFGMEKRRVPGDGFVTGAGKVFGRPVFLFSHDFTVFGGSLSGSNAAK VVRIMEEAAKIGVPVIGFNDSGGARIQEGVDSLAGYADIFLRNTLFSGVIPQISVIMGPC AGGAVYSPAITDFTFMVDNSSYMFVTGPEVVSVVGGKLVTKDELGGPHVHATKSGVSAGT FPNDIVAMAQLRRLYSYLPLSNRDSAPVVPTADNRYRDVSSLNTVVPVEVKEAYDMRDAI FPVIDQDSFFEIQPQFAKNIICGFARVEGRSVCIIANQPKVQAGVLDIDSSVKAARMVRF ADAFNIPIITFVDVPGFLPGVQQEYGGIIRHGAKLLYAYAEATVPKVTIITRKAYGGAYD VMSSKHLRGDGNYAWPRAEIAVMGAAGACKLLYSKETAEQQAQRIADYEKAFCTPLSAAR KGFVDAVIDPSETRMRVCEDLERLSRKQLRNPWKKHGNIPL |
| DNA Sequence | >LmjF28.0490 |||propionyl-coa carboxylase beta chain, putative|Leishmania major|chr 28|||Manual ATGCGCCGGC TGGGATGTGG GCTGGTTTCG GCGGCCATGG GCGTGGGCGG GATGAGCTCCTCGCAGCTAA CGAAGGAGTT TGACCGCCAC ACACACACGC CGACCGCCGC TGAGGAGTCACGCCACAAGA AGAAGCGCCT GACTGCCATG GAGCGCGTGC AGCTCTTCTG CGACCCCGGCACGTTCCGCG AGCGTGATGC CCTGGTTGAG CACGGGTGCC ACAATTTCGG CATGGAGAAGAGGAGGGTGC CTGGTGACGG CTTCGTTACG GGTGCGGGCA AGGTCTTTGG CCGCCCTGTGTTTCTCTTCT CGCACGACTT CACGGTGTTC GGCGGCTCTC TCTCCGGCTC CAACGCCGCCAAAGTGGTGC GCATCATGGA GGAGGCCGCC AAGATCGGTG TGCCAGTGAT CGGCTTCAACGACTCCGGCG GTGCTCGCAT CCAAGAGGGT GTGGACTCGC TGGCTGGCTA CGCTGACATTTTCCTCCGCA ACACGCTCTT CTCCGGTGTC ATTCCACAAA TCTCCGTGAT CATGGGGCCTTGTGCCGGCG GTGCCGTCTA CTCGCCCGCC ATCACCGACT TCACGTTCAT GGTGGACAACTCGTCCTACA TGTTTGTGAC GGGTCCGGAG GTGGTGAGTG TCGTGGGCGG CAAGCTAGTGACCAAGGACG AACTCGGCGG TCCGCATGTG CACGCGACCA AGTCGGGTGT GTCGGCGGGTACATTCCCGA ATGACATCGT TGCGATGGCG CAGCTCCGCC GCCTGTACTC GTATCTGCCATTGAGCAACC GCGACTCTGC CCCGGTTGTG CCGACGGCCG ACAACCGCTA CCGTGACGTCTCCTCGCTGA ACACCGTCGT GCCGGTGGAG GTGAAGGAGG CGTACGATAT GCGCGACGCCATCTTCCCTG TGATCGACCA AGACTCCTTC TTCGAGATAC AGCCGCAGTT CGCCAAGAACATCATCTGCG GTTTCGCCCG CGTTGAGGGC CGCTCCGTGT GCATCATTGC GAACCAACCGAAGGTGCAGG CTGGCGTGCT CGATATCGAC TCCTCCGTCA AGGCTGCGCG CATGGTGCGTTTTGCCGATG CCTTCAACAT CCCGATCATC ACCTTCGTAG ACGTCCCCGG ATTCTTGCCTGGCGTGCAGC AGGAATACGG CGGCATCATT CGCCACGGCG CGAAGCTGCT GTACGCCTACGCTGAGGCGA CTGTCCCGAA GGTGACAATC ATCACGCGCA AGGCGTACGG TGGCGCCTACGATGTGATGA GCTCGAAACA CCTCCGTGGC GACGGCAACT ACGCCTGGCC GCGCGCCGAGATTGCCGTCA TGGGCGCCGC CGGTGCATGC AAGCTTCTGT ACTCGAAGGA GACGGCGGAGCAGCAGGCGC AGCGTATCGC CGATTACGAG AAGGCCTTCT GCACACCGCT GTCGGCCGCTCGAAAGGGAT TTGTGGATGC GGTGATTGAC CCCAGCGAGA CCCGCATGCG CGTGTGCGAGGACCTGGAGC GCCTGTCCCG CAAGCAGCTC CGCAATCCAT GGAAGAAGCA CGGCAACATTCCGCTGTAA |
| Propionyl-coa carboxylase beta chain, putative Q4Q8M1] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ligase activity |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K01966 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | propionyl Coenzyme A carboxylase, beta polypeptide [Homo sapiens] | 62 | 1e-173 | 605 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| accD4 PROBABLE PROPIONYL-CoA CARBOXYLASE BETA CHAIN 4 ACCD4 (PCCASE) (PROPANOYL-COA:CARBON DIOXIDE LIGASE) | Mycobacterium tuberculosis H37Rv | 45% | 1e-110 | 392 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC50980 | Acetyl-coenzyme A carboxyltransferase domain profiles | 40.197 | 21-235 | PS50980 |
| PDOC50980 | Acetyl-coenzyme A carboxyltransferase domain profiles | 76.216 | 236-514 | PS50989 |
| PDOC00016 | Cell attachment sequence | 429-431; | PS00016 | |
| Acylation | N-myristoylation site | 7-12; 16-21; 17-22; 111-116; 112-117; 116-121; 236-241; 240-245; 445-450; | PS00008 | |
| Glycosylation | N-glycosylation site | 140-143; 200-203; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 45-48; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 35-38; 48-51; 61-64; 207-210; 221-224; 262-265; 309-312; 371-374; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 61-63; 262-264; 352-354; 412-414; 424-426; 506-508; | PS00005 | |
| Propionyl-coa carboxylase beta chain, putative [Q4Q8M1] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1vrgF | |
| Percent Identity | 55% | |
| Target Region | 1-522 | |
| Template Region | 1-515 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| GLY | 183 | Backbone amide |
| GLY | 184 | Backbone amide |
| GLY | 418 | Backbone amide |
| ALA | 419 | Backbone amide |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 71-52-3 | BICARBONATE ION | 61.017 | C H O3 | [O-]C(=O)O | 1vrg |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 1vrg |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/2124352/Q4Q8M1.pdb 2.0 522 = residues | | = | +| Ramachandran plot: 93.0% core 6.3% allow 0.7% gener 0.0% = disall | | = | +| All Ramachandrans: 7 labelled residues (out of 520) = | | Chi1-chi2 plots: 0 labelled residues (out of 276) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.6 Bad contacts: = 4 | *| Bond len/angle: 10.0 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.12 Covalent: -0.14 Overall: = 0.02 | | = | | M/c bond lengths: 99.2% within limits 0.8% highlighted = | *| M/c bond angles: 93.8% within limits 6.2% highlighted 1 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |