| Serine/threonine protein phosphatase catalytic subunit, putative [Q4Q8K1] | |
|---|---|
| Systematic Name | LmjF.28.0690 [Leishmania major] |
| Gene Name | LMJF_28_0690 |
| Molecular Weight | 34402 Da |
| Protein Sequence Size | 301 |
| Function | |
| Charge | -3.5 |
| Isoelectric Point | 5.4488 pH |
| Description | Serine/threonine protein phosphatase catalytic subunit, putative (EC 3.1.3.16). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 3.1.3.16 |
| Sequence | >tr|Q4Q8K1|Q4Q8K1_LEIMA Serine/threonine protein phosphatase catalytic subunit, putative (EC 3.1.3.16) - Leishmania major SVDTIIEQLLEVRGAKPGKQVQLAENDVKQLAIRTREILLSQPPLLELEAPIKICGDIHG QYYDLIRLFENGGFPPTANYLFLGDYVDRGKQGLETICLVFAFKVKFPENFFILRGNHEC ASINRIYGFFDECKRRYNIRLWKAFTDTFNCLPVACIIDDKIFCCHGGLSPELQTMDQIK KITRPCDVADTGLICDLLWSDPEEGLSGWGENDRGVSFTFGQDIVEKFLNKHQFELICRA HQVVEDGYQFFAKRKLITIFSAPNYCNEFDNSGAVMTVDNELMCSFQILKPSVKKPKFYS |
| DNA Sequence | >LmjF28.0690 |||serine/threonine protein phosphatase catalytic subunit, putative|Leishmania major|chr 28|||Manual ATGAGCGTCG ACACCATTAT CGAGCAGCTG CTGGAGGTGC GTGGCGCGAA GCCGGGCAAGCAGGTGCAGT TAGCCGAGAA TGACGTGAAG CAGCTGGCGA TACGCACGCG CGAGATTCTTCTCTCGCAGC CGCCTCTGCT TGAGCTGGAG GCGCCCATCA AGATCTGCGG TGACATTCACGGCCAGTACT ACGACCTTAT CCGTCTTTTC GAGAACGGCG GCTTCCCGCC TACAGCGAACTACCTCTTCC TCGGCGACTA CGTGGACCGC GGAAAGCAGG GTCTCGAGAC GATCTGCCTGGTCTTCGCCT TCAAGGTGAA GTTCCCCGAA AACTTCTTCA TCCTTCGAGG TAACCACGAGTGCGCCAGCA TCAATCGCAT CTACGGCTTC TTCGACGAGT GCAAGCGCCG CTACAACATTCGCCTCTGGA AGGCGTTCAC AGATACGTTC AACTGCCTTC CTGTGGCGTG CATTATCGATGACAAGATCT TCTGCTGCCA TGGTGGTCTT TCGCCGGAGC TGCAAACGAT GGACCAGATCAAGAAGATCA CGCGCCCGTG CGATGTGGCG GACACTGGTC TGATCTGCGA CTTGCTGTGGTCAGACCCGG AGGAAGGACT CTCGGGGTGG GGCGAGAACG ACCGTGGCGT GTCCTTCACTTTCGGTCAGG ATATTGTAGA GAAGTTCTTG AACAAGCACC AGTTTGAGCT GATTTGCCGCGCGCACCAAG TCGTAGAGGA TGGGTACCAG TTCTTCGCGA AGCGCAAGCT CATCACCATCTTCTCTGCCC CTAACTACTG TAATGAGTTC GATAACAGCG GCGCAGTGAT GACCGTCGACAACGAGCTCA TGTGCTCCTT CCAGATCCTT AAGCCGTCTG TGAAGAAGCC GAAGTTCTACTCGTAA |
| Serine/threonine protein phosphatase catalytic subunit, putative Q4Q8K1] | |
|---|---|
| Metabolite Information | |
| Molecular Function | hydrolase activity; protein phosphatase type 1 activity |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K06269 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | protein phosphatase 1, catalytic subunit, gamma isoform [Homo sapiens] | 75 | 1e-142 | 501 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YER133w serthr phosphoprotein phosphatase 1, catalytic chain | Saccharomyces cerevisiae | 72% | 1e-137 | 481 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00115 | Serine/threonine specific protein phosphatases signature | 115-120; | PS00125 | |
| PDOC00002 | The rule PS00002 has been deleted from the PROSITE database | 208-211; | PS00002 | |
| Acylation | N-myristoylation site | 15-20; 117-122; 216-221; 274-279; | PS00008 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 181-184; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 5-8; 201-204; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 293-295; | PS00005 | |
| Serine/threonine protein phosphatase catalytic subunit, putative [Q4Q8K1] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2o8gB | |
| Percent Identity | 75% | |
| Target Region | 1-301 | |
| Template Region | 6-295 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 89 | Sidechain |
| ARG | 90 | Sidechain |
| ASN | 118 | Sidechain |
| HIS | 119 | Sidechain |
| ARG | 215 | Sidechain |
| HIS | 242 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 16397-91-4 | MANGANESE (II) ION | 54.938 | Mn | [Mn+2] | 2o8g |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1619316/Q4Q8K1.pdb 2.0 301 = residues | | = | *| Ramachandran plot: 90.9% core 7.2% allow 1.5% gener 0.4% = disall | | = | +| All Ramachandrans: 7 labelled residues (out of 299) = | +| Chi1-chi2 plots: 1 labelled residues (out of 199) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 1 | *| Bond len/angle: 5.5 Morris et al class: 1 = 1 2 | +| 3 cis-peptides = | | G-factors Dihedrals: 0.05 Covalent: -0.07 Overall: = 0.01 | | = | | M/c bond lengths: 99.5% within limits 0.5% highlighted = | | M/c bond angles: 94.9% within limits 5.1% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |