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Ribonucleoside-diphosphate reductase large chain, putative [Q4Q8H8]
Systematic NameLmjF.28.0890 [Leishmania major]
Gene NameLMJF_28_0890
Molecular Weight90728 Da
Protein Sequence Size799
Function
Charge5
Isoelectric Point7.157 pH
DescriptionRibonucleoside-diphosphate reductase large chain, putative (EC 1.17.4.1).
Subcellular Locationnucleus; ribonucleoside-diphosphate reductase complex[Predict]
E. C. Number 1.17.4.1
Sequence>tr|Q4Q8H8|Q4Q8H8_LEIMA Ribonucleoside-diphosphate reductase large chain, putative (EC 1.17.4.1) - Leishmania major.
SDSAPMIIKRNGEAQPYDASKIRRRFERVMEGLDRDHLDVDMLTENVTRGLTDQIRYDKL
DELVAQTAAYSVTKHPDYGRMGGRLCTTSLHKQTNESLLETFRLLHSHVTVQTNRPAPLI
ADDVWEVMKQHHAELQGMIDYSRDLNFEFFGYKTLERSYLLRIETGRGEMRIVERPQQMF
MRVALGIHGADLKNVRETYDLMSKGFFTHATPTLFNAGTPKPQMSSCFLVASKEDSIDGI
YDTLKECAVISKAAGGIGLHVHNIRAAGSYIAGTNGTSNGLVPMLRVFNNTARYVDQGGG
KRKGAFAVYLEPWHADVFGFLLLKRNTGKDDQRARDLFYALWIPDLFMRRVKSEGKWTLM
DPNTCPGLSDCYGEEFERLYERYESEGRGVKTIQAQEVWFAILESQIETGGPFILYKDAC
NSKSNQKNLGTIKCSNLCTEIVEYTSSQETAVCNLASIALPRFVEVEKRELNHQLLYTVT
KQITRNLNRVIDRNYYPVETARRSNMRNRPIGLGVQGLADAFILMRLPFVSAEAQRVNAE
IFETIYFAAVESSMELAKEEGPYETFAGSPASEGILQFDMWKNARPNSGRWDWASLKEKV
MQVGMRNSLLVAPMPTASTSQILGNNECFEPFTSNIYVRRVLSGEFPVVNKYLVMDLIAR
GLWNESMRNEIISYNGSILAINGMPDDLRELYRTVWEIPQRKLIDMARDRGQYIDQSQSL
NLFLQSPTSGQITSMLFYSWEAGLKTGVYYLRTKAAADAIKFTVDAKRMKEIQERETSQQ
TMSSHRASQPEECLNCGS
DNA Sequence>LmjF28.0890 |||ribonucleoside-diphosphate reductase large chain, putative|Leishmania major|chr 28|||Manual
ATGTCCGATT CGGCGCCGAT GATCATCAAG CGCAATGGCG AGGCGCAACC GTACGATGCTTCCAAGATCC GCCGCCGCTT CGAGCGGGTG ATGGAGGGGC TCGACCGCGA CCATCTCGACGTGGACATGC TGACGGAGAA CGTGACGCGC GGCTTGACGG ACCAGATCCG CTACGACAAGCTGGACGAGC TCGTGGCGCA GACGGCGGCG TACTCGGTCA CAAAGCACCC AGACTACGGCCGCATGGGCG GTCGCCTGTG CACGACATCA CTGCACAAGC AGACGAATGA GTCGCTGCTGGAGACGTTCC GCCTTCTGCA CAGCCACGTC ACGGTGCAGA CAAACCGTCC CGCTCCGCTGATCGCCGACG ATGTGTGGGA GGTTATGAAG CAGCACCACG CGGAGCTGCA GGGCATGATTGACTACTCCC GCGATTTGAA CTTCGAGTTC TTCGGGTACA AGACGCTTGA GCGCTCGTACCTGCTGCGTA TCGAGACTGG AAGGGGTGAG ATGCGAATTG TGGAGCGTCC ACAGCAGATGTTCATGCGTG TCGCTCTCGG CATTCACGGC GCAGATCTCA AGAACGTGCG TGAGACGTACGACCTCATGT CCAAGGGCTT CTTCACCCAC GCCACCCCGA CCCTCTTTAA CGCCGGCACCCCGAAGCCGC AAATGAGCTC GTGCTTCCTG GTGGCGTCGA AGGAAGACAG CATCGACGGCATCTACGACA CGCTGAAGGA GTGCGCCGTG ATCAGCAAGG CGGCCGGCGG CATTGGCCTGCACGTGCACA ACATCCGCGC GGCTGGCAGC TACATAGCTG GTACGAACGG CACGTCGAACGGGCTCGTGC CAATGCTACG CGTCTTCAAC AACACCGCCC GCTACGTGGA CCAGGGGGGCGGTAAGCGCA AGGGCGCCTT TGCCGTCTAT CTTGAGCCCT GGCACGCCGA CGTCTTTGGCTTTCTGCTGC TGAAACGAAA CACGGGCAAA GATGACCAGC GCGCCCGCGA CCTCTTCTATGCGCTGTGGA TCCCAGATCT ATTTATGAGG CGTGTGAAAA GCGAGGGGAA GTGGACGCTGATGGATCCCA ACACGTGCCC GGGCCTCAGC GATTGCTATG GTGAGGAGTT TGAGCGTCTCTACGAGCGCT ACGAGTCCGA GGGCCGCGGC GTCAAGACGA TCCAGGCCCA GGAGGTGTGGTTTGCCATCC TGGAGTCGCA GATCGAGACG GGCGGGCCGT TCATCCTCTA CAAGGACGCATGCAACAGCA AGTCGAACCA GAAGAATCTT GGCACGATCA AGTGCTCGAA CCTATGCACGGAGATTGTCG AGTACACGTC GTCGCAGGAG ACGGCGGTGT GCAACCTCGC CTCCATCGCGCTGCCCCGCT TCGTCGAGGT TGAGAAGCGC GAGCTGAACC ACCAGCTTCT CTACACCGTGACAAAGCAGA TCACACGCAA CCTCAACCGC GTTATTGACC GCAACTACTA CCCGGTCGAGACGGCGCGCC GCAGTAACAT GCGCAACCGC CCCATCGGCC TCGGCGTTCA GGGGCTCGCGGACGCCTTCA TCCTCATGCG GCTGCCGTTT GTCTCGGCGG AGGCGCAGCG GGTGAACGCAGAGATCTTCG AAACGATCTA CTTTGCTGCG GTCGAGTCGT CGATGGAGCT GGCGAAGGAGGAGGGCCCGT ACGAGACCTT CGCGGGCAGC CCGGCCAGCG AGGGCATTCT GCAGTTCGACATGTGGAAGA ATGCGCGGCC CAACAGCGGT CGCTGGGACT GGGCCAGCCT CAAGGAGAAGGTGATGCAGG TTGGCATGCG CAACTCCCTG CTGGTCGCCC CCATGCCAAC CGCGTCCACCTCGCAGATTC TCGGCAACAA CGAGTGCTTC GAGCCCTTCA CCTCGAACAT CTACGTCCGCCGTGTGCTGA GCGGCGAGTT CCCTGTTGTG AACAAGTACC TCGTCATGGA CCTCATCGCGCGTGGTCTAT GGAACGAGTC GATGCGGAAC GAGATCATCT CTTACAATGG CTCTATCCTCGCTATTAACG GCATGCCGGA CGACCTGAGG GAGCTTTACC GGACCGTGTG GGAGATTCCGCAGCGCAAGC TGATAGATAT GGCACGCGAC CGCGGCCAGT ACATTGACCA GTCGCAGTCGCTGAACCTGT TCCTCCAGAG CCCCACCTCC GGCCAGATCA CGTCCATGCT CTTCTATTCCTGGGAGGCCG GACTGAAGAC TGGCGTCTAC TACCTGCGCA CGAAGGCCGC GGCGGACGCGATCAAGTTCA CCGTGGACGC GAAGCGAATG AAGGAGATTC AGGAGAGGGA AACTTCGCAGCAGACCATGT CCTCGCACCG CGCTTCGCAG CCCGAGGAGT GCCTGAACTG CGGATCCTAA
Ribonucleoside-diphosphate reductase large chain, putative Q4Q8H8]
Metabolite Information
Molecular Functionribonucleoside-diphosphate reductase activity
Biochemical PathwayDNA replication
Regulatory Pathway
KEGG PathwaysK00525
Orthologs
Homologs GI Percent Identity Evalue Score
Homo sapiensribonucleoside-diphosphate reductase M1 chain [Homo sapiens]560920
DEG Information
DEG Protein DEG Organism Percent Identity Evalue Bit Score
YER070w ribonucleoside-diphosphate reductase, large subunitSaccharomyces cerevisiae61%0970
Post Translational Modification
PTM Type PTM Sub Type Score Modification Site Prosite ID
PDOC51161ATP-cone domain profile16.4401-06-1997 00:00:00PS51161
PDOC00076Multicopper oxidases signatures590-610; PS00079
PDOC00084Ribonucleotide reductase large subunit signature594-616; PS00089
PDOC00015The rule PS00015 has been deleted from the PROSITE database and replaced by the following motif: PS50079 (Bipartite nuclear localization signal profile10-26; PS00015
AcylationN-myristoylation site83-88; 187-192; 240-245; 269-274; 274-279; 277-282; 368-373; 431-436; 518-523; 569-574; 605-610; 625-630; 677-682; 731-736; 744-749; PS00008
AmidationAmidation site300-303; PS00009
GlycosylationN-glycosylation site47-50; 96-99; 276-279; 290-293; 665-668; 676-679; PS00001
PhosphorylationcAMP- and cGMP-dependent protein kinase phosphorylation site325-328; PS00004
PhosphorylationCasein kinase II phosphorylation site98-101; 233-236; 244-247; 328-331; 359-362; 406-409; 447-450; 553-556; 596-599; 695-698; 789-792; PS00006
PhosphorylationProtein kinase C phosphorylation site102-104; 114-116; 166-168; 220-222; 244-246; 292-294; 328-330; 432-434; 501-503; 589-591; 596-598; 667-669; 785-787; PS00005
PhosphorylationTyrosine kinase phosphorylation site10-18; 50-58; 154-160; 334-340; 490-497; PS00007
SulfationTyrosine sulfation site235-249; 366-380; 438-452; 557-571; PS00003
Ribonucleoside-diphosphate reductase large chain, putative [Q4Q8H8]
Model Information
Template PDB ID2eudA
Percent Identity61%
Target Region95-760
Template Region90-648
Domain Information
Domains Start End
Active Site Information
Residue Active Site Number Functional Part
CYS134Sidechain
ASN343Sidechain
CYS345Sidechain
GLU347Sidechain
CYS360Sidechain
Co-Factor
Metal Description
Ligands
CAS number Name Mol. Weight Mol. Formula Smile Notation PDB Reference
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER506.196C10 H17 N6 O12 P3O=P(O)(O)NP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O2eud
GEMCITABINE DIPHOSPHATE423.158C9 H13 F2 N3 O10 P2O=P(O)(O)OP(=O)(O)OCC2OC(N1C(=O)N=C(N)C=C1)C(F)(F)C2O2eud
7791-18-6MAGNESIUM ION24.305Mg[Mg+2]2eud
Mutational Information
Residue Feature Description
Modeled Protein Template Structure
+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/3867750/Q4Q8H8.pdb 2.0 661 = residues | | = | *| Ramachandran plot: 93.0% core 6.3% allow 0.3% gener 0.3% = disall | | = | +| All Ramachandrans: 15 labelled residues (out of 659) = | +| Chi1-chi2 plots: 5 labelled residues (out of 413) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 11.4 Bad contacts: = 8 | *| Bond len/angle: 6.1 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.13 Covalent: -0.12 Overall: = 0.04 | | = | | M/c bond lengths: 99.4% within limits 0.6% highlighted = | *| M/c bond angles: 94.8% within limits 5.2% highlighted 1 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further.
Overlapped Structure Procheck Summary
LeishBase: Leishmania Structural Database
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