| Glucose-regulated protein 78, putative [Q4Q8E6] | |
|---|---|
| Systematic Name | LmjF.28.1200 [Leishmania major] |
| Gene Name | LMJF_28_1200 |
| Molecular Weight | 71941 Da |
| Protein Sequence Size | 658 |
| Function | |
| Charge | -17 |
| Isoelectric Point | 4.7866 pH |
| Description | Glucose-regulated protein 78, putative. |
| Subcellular Location | endoplasmic reticulum[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4Q8E6|Q4Q8E6_LEIMA Glucose-regulated protein 78, putative - Leishmania major TRKDNLTLMAVCLVSAILVVSAAAVPDGSGKVESPCIGVDLGTTYSVAGVWQKGEVHIVT NEMGNRITPSVVAFTDAERLVGDGAKNQLPQNPENTIYAIKRLIGRKYADPTVQNDKKLL SYHIVADKTGKPLVQVTVKGQQKRFTPEEVSAMVLQKMKEISETFLGEKVKNAVVTVPAY FNDAQRQATKDSGKIAGLNVVRIINEPTAAAIAYGLNKAGEKNILVFDLGGGTFDVSLLT IDEGFFEVVATNGDTHLGGEDFDNNMMKFFVDGLKRKQNVDISNDQKALARLRKACEAAK RQLSSHPEARVEVDSLVEGYDFSEKITRAKFEELNMALFKNTLVPVQKVLEDAKLKKSDI DEIVLVGGSTRIPKVQQLIKDFFSGKEPNKGINPDEAVAYGAAVQAAVLTGESEVGGKVV LVDVIPLSLGIETVGGVMTKLIERNTQIPTKKSQIFSTYQDNQPSVLIQVFEGERGMTKD NRLLGKFDLSGIPPAPRGVPQIEVAFDVDENSILQVTASDKSSGKREEITITNDKGRLSE EEIERMVREAAEFEDEDRKVRERVEAKNSLESIAYSLRNQINDKDKLGDKLDADDKKAIE EAVKDALDFVDENPNADREEFEAARTKLQSVTNPIIQKVYQGTAGSGAEEADAMDDL |
| DNA Sequence | >LmjF28.1200 |||glucose-regulated protein 78, putative|Leishmania major|chr 28|||Manual ATGACGCGGA AAGACAACCT AACACTCATG GCCGTGTGCC TCGTGTCCGC CATACTGGTGGTGTCGGCTG CGGCTGTCCC TGATGGCAGC GGCAAGGTGG AGTCGCCGTG CATCGGCGTTGACCTGGGTA CGACGTACTC GGTTGCCGGT GTTTGGCAGA AGGGTGAGGT CCACATTGTCACGAACGAGA TGGGCAACCG CATCACCCCG TCTGTGGTCG CCTTCACCGA CGCGGAGCGTCTGGTCGGCG ATGGCGCGAA GAATCAGTTG CCGCAGAACC CGGAGAACAC CATCTACGCCATCAAGCGTC TCATCGGCCG CAAATACGCG GACCCCACCG TTCAGAATGA CAAGAAGCTGCTCTCGTACC ATATTGTGGC GGACAAAACT GGCAAGCCGC TCGTGCAGGT GACGGTGAAGGGCCAGCAGA AGCGGTTCAC GCCTGAGGAG GTGAGCGCCA TGGTGCTACA GAAGATGAAAGAGATCTCTG AGACCTTCCT CGGCGAGAAG GTGAAGAACG CCGTCGTAAC GGTGCCAGCGTACTTTAATG ATGCGCAGCG CCAGGCAACG AAGGACTCCG GTAAGATCGC CGGCCTTAACGTCGTCCGCA TCATCAACGA GCCCACAGCG GCGGCCATAG CGTACGGGCT CAACAAAGCTGGCGAGAAGA ACATCCTGGT CTTCGACCTC GGTGGCGGCA CGTTCGATGT GTCGCTGCTGACAATCGACG AGGGTTTCTT CGAGGTGGTC GCGACGAACG GTGATACCCA CCTCGGTGGTGAGGACTTCG ATAACAACAT GATGAAGTTT TTCGTGGACG GCCTCAAGCG CAAGCAGAACGTCGACATCT CGAATGACCA GAAGGCCCTG GCCCGTCTGC GCAAGGCATG CGAGGCTGCGAAGCGTCAGT TGTCGTCCCA CCCGGAGGCG CGCGTAGAGG TGGACAGCCT TGTCGAGGGCTACGACTTCA GCGAGAAGAT CACGCGGGCC AAGTTCGAGG AGCTGAACAT GGCCCTCTTCAAGAACACGC TGGTGCCTGT GCAGAAAGTG CTAGAGGATG CAAAGCTGAA GAAGAGTGACATCGACGAGA TCGTCCTGGT CGGCGGCTCG ACCCGAATTC CGAAGGTGCA ACAGCTCATCAAGGACTTCT TCAGCGGCAA GGAGCCGAAC AAGGGCATCA ACCCCGACGA GGCCGTCGCGTACGGCGCTG CGGTGCAGGC GGCCGTGCTG ACGGGCGAGA GCGAGGTGGG CGGCAAGGTCGTGCTCGTTG ATGTGATTCC CTTGTCTCTC GGCATCGAGA CCGTTGGCGG AGTCATGACGAAGCTGATCG AGCGCAACAC GCAGATCCCG ACCAAGAAGA GCCAGATCTT CTCCACCTATCAGGACAACC AACCCAGCGT GCTGATTCAG GTCTTCGAGG GCGAGCGCGG GATGACGAAAGACAACCGCT TGCTTGGCAA GTTCGACCTC TCCGGCATCC CGCCGGCGCC GCGCGGCGTGCCGCAGATCG AGGTCGCCTT CGATGTGGAC GAGAACAGCA TCCTGCAGGT GACGGCGAGCGACAAGTCCT CTGGAAAGCG GGAGGAGATC ACCATCACAA ATGACAAGGG CCGCCTCAGCGAGGAGGAGA TCGAGCGCAT GGTGCGCGAG GCTGCCGAGT TCGAGGATGA GGACCGTAAGGTGCGCGAGC GTGTCGAAGC GAAGAACTCG CTAGAGAGCA TCGCGTACTC CCTTCGCAACCAGATCAACG ACAAGGACAA GCTTGGTGAC AAGCTCGACG CGGACGACAA GAAGGCGATCGAGGAGGCTG TGAAGGATGC CCTCGACTTT GTCGACGAGA ACCCCAATGC AGACCGTGAGGAGTTCGAGG CAGCACGCAC GAAGCTGCAG AGTGTGACGA ACCCCATCAT TCAAAAGGTGTACCAGGGCA CCGCCGGCTC TGGTGCAGAA GAGGCGGACG CGATGGATGA CTTGTAG |
| Glucose-regulated protein 78, putative Q4Q8E6] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; molecular function unknown |
| Biochemical Pathway | SRP-dependent cotranslational protein targeting to membrane; protein folding |
| Regulatory Pathway | |
| KEGG Pathways | K09490 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) [Homo sapiens] | 59 | 0 | 718 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YJL034w nuclear fusion protein | Saccharomyces cerevisiae | 57% | 0 | 687 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00269 | Heat shock hsp70 proteins family signatures | 40-47; | PS00297 | |
| PDOC00269 | Heat shock hsp70 proteins family signatures | 227-240; | PS00329 | |
| PDOC00269 | Heat shock hsp70 proteins family signatures | 364-378; | PS01036 | |
| Acylation | N-myristoylation site | 39-44; 43-48; 216-221; 436-441; 643-648; 648-653; | PS00008 | |
| Amidation | Amidation site | 105-108; 524-527; | PS00009 | |
| Glycosylation | N-glycosylation site | 6-9; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 144-147; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 2-5; 76-79; 147-150; 241-244; 252-255; 306-309; 316-319; 359-362; 385-388; 459-462; 518-521; 540-543; 647-650; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 2-4; 30-32; 130-132; 138-140; 193-195; 324-326; 370-372; 385-387; 451-453; 520-522; 524-526; 577-579; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 568-576; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 314-328; | PS00003 | |
| Glucose-regulated protein 78, putative [Q4Q8E6] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1yuwA | |
| Percent Identity | 63% | |
| Target Region | 33-586 | |
| Template Region | 2-554 | |
| Domain Information | ||
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| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| LYS | 70 | Sidechain |
| Co-Factor | |
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| Metal | Description |
| Ligands | |||||
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| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| Mutational Information | ||
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| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/626418/Q4Q8E6.pdb 2.0 551 = residues | | = | *| Ramachandran plot: 89.9% core 8.4% allow 1.2% gener 0.4% = disall | | = | *| All Ramachandrans: 21 labelled residues (out of 549) = | +| Chi1-chi2 plots: 3 labelled residues (out of 328) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 6.9 Bad contacts: = 4 | *| Bond len/angle: 7.2 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.01 Covalent: -0.15 Overall: = -0.04 | | = | | M/c bond lengths: 99.6% within limits 0.4% highlighted = | | M/c bond angles: 93.6% within limits 6.4% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |