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DNA polymerase kappa, putative [Q4Q8C4]
Systematic NameLmjF.28.1420 [Leishmania major]
Gene NameLMJF_28_1420
Molecular Weight67200 Da
Protein Sequence Size598
Function
Charge17.5
Isoelectric Point8.8 pH
DescriptionDNA polymerase kappa, putative (Dna polymerase iv, putative) (EC 2.7.7.7).
Subcellular LocationN.A.[Predict]
E. C. Number 2.7.7.7
Sequence>tr|Q4Q8C4|Q4Q8C4_LEIMA DNA polymerase kappa, putative (Dna polymerase iv, putative) (EC 2.7.7.7) - Leishmania major
SPAPRGRYSDSHTEEIAADCAALEDHGRDAPQDSHHPQNHAGMNGAQHYDHRSAGKLNFD
ASKAGLQQVDKDYVEHVIQEASKGSAFYQKEQRLEETRRRKAEELLEKAKTFDSISAAEK
QRIKAMVDTMVDELEATRDLRRRYIHIDMDMFYAAVEEKKTPSLRGKPFGVGSQQMLSTT
NYIARQYGVRSGMPGFIGKKLCPELIIVPNDFPAYQREAARVHSIASRYDAQFVSVGLDE
LTMDVTKYLQEFPAVSASDIAHDFRDEVFLKTQLTSSGGIGPTSILAKIASNVNKPNGQH
EITLLTREEVINYVRDIPLRKIPGIGYAQEMTLGALHIHTCGGLLEHKYLLAYLFREKTL
AHYLSVGLGLAETFSLRKHQARQSVGKETSFSEPLPSPEAFTRLFRKLLEQCHVRCVRDH
LQPRKMTLVLKYRTYDTEQFSVTLPSHTNDLKVWLEASQKLLEPHLLHYAELRLIGVRLQ
RFTDIDDDHAGLNSTRDALTGALTEPLQVNDDLDMDADDADRDAEKSGIAAKQSDPYGES
PAQPTAAHKRRIPVSKFLTDPNIPKARPAEPAAVPAKAAKPARRRTKPSKRKLHKKK
DNA Sequence>LmjF28.1420 |||DNA polymerase kappa, putative|Leishmania major|chr 28|||Manual
ATGTCGCCTG CCCCGCGAGG TCGCTACAGC GACTCGCACA CGGAGGAAAT CGCGGCGGACTGCGCCGCCC TCGAAGATCA CGGCCGCGAC GCGCCGCAGG ACTCGCATCA TCCGCAGAACCACGCCGGCA TGAACGGTGC ACAGCACTAT GACCACCGCA GCGCTGGCAA ACTCAACTTTGATGCCTCGA AGGCAGGGCT TCAGCAAGTA GATAAAGACT ACGTCGAGCA CGTCATTCAGGAGGCGTCGA AGGGGTCTGC GTTCTACCAA AAGGAGCAGC GGCTCGAGGA GACGCGGCGTCGCAAAGCCG AGGAGCTGCT GGAGAAGGCC AAGACATTCG ACTCCATCAG CGCCGCCGAGAAGCAGCGGA TCAAGGCCAT GGTAGACACC ATGGTGGACG AGCTCGAGGC GACGCGCGATCTCCGGCGCC GGTACATCCA CATCGACATG GACATGTTCT ACGCTGCGGT GGAGGAGAAGAAGACGCCGT CGCTGAGAGG CAAGCCCTTC GGCGTCGGCT CTCAGCAGAT GCTCTCCACGACAAACTACA TTGCGCGGCA GTACGGTGTG CGCTCTGGCA TGCCCGGCTT CATCGGCAAGAAGCTCTGCC CTGAGCTCAT TATCGTGCCA AACGACTTCC CAGCCTACCA GCGAGAGGCGGCGCGGGTGC ACAGCATCGC CTCCCGCTAC GATGCGCAGT TCGTCAGCGT CGGCCTCGATGAACTGACGA TGGACGTGAC AAAGTACCTG CAGGAGTTCC CGGCCGTTTC AGCCTCTGACATCGCCCACG ACTTCCGCGA CGAGGTGTTC CTCAAGACAC AGCTTACAAG CAGCGGCGGCATCGGACCCA CGTCGATTCT CGCGAAGATT GCGAGTAACG TCAACAAGCC CAATGGTCAGCACGAGATCA CGCTGCTGAC GCGAGAGGAA GTTATCAACT ACGTGCGCGA CATTCCGCTGCGCAAGATCC CTGGCATCGG CTACGCGCAA GAGATGACAC TCGGTGCGCT GCACATCCACACCTGCGGCG GCCTCCTGGA GCACAAGTAC CTCCTAGCCT ACCTGTTCCG AGAAAAGACTCTGGCGCACT ACCTCAGTGT TGGACTGGGG CTGGCAGAGA CGTTCTCGCT GCGCAAGCATCAGGCACGGC AGTCGGTTGG TAAGGAAACG TCGTTTAGCG AGCCACTGCC CTCGCCAGAGGCCTTCACTC GACTCTTCCG CAAGCTGCTT GAGCAGTGCC ATGTCCGCTG CGTTCGCGATCACCTGCAGC CTCGCAAGAT GACACTGGTT CTCAAGTATC GCACCTATGA CACGGAGCAGTTCAGTGTGA CGCTCCCGAG CCACACAAAC GACCTCAAGG TGTGGCTCGA GGCCTCGCAGAAGCTGCTGG AGCCGCATCT TCTGCACTAT GCCGAGCTCC GCCTGATCGG CGTCCGCCTGCAGCGCTTCA CAGATATTGA TGATGACCAT GCTGGCCTCA ACAGCACCCG CGATGCCTTGACGGGCGCCT TGACAGAACC GCTTCAAGTG AATGACGACT TAGATATGGA TGCCGACGATGCCGACCGTG ACGCTGAGAA GAGCGGGATA GCTGCGAAGC AGTCAGACCC GTATGGAGAGTCGCCGGCGC AGCCTACCGC AGCGCACAAG CGCAGGATTC CCGTCTCGAA GTTCCTGACGGATCCAAACA TCCCGAAAGC CCGCCCTGCA GAGCCCGCTG CGGTGCCCGC CAAGGCCGCCAAGCCGGCGC GCAGGCGCAC GAAGCCGTCG AAGCGAAAGC TGCACAAAAA GAAGTGA
DNA polymerase kappa, putative Q4Q8C4]
Metabolite Information
Molecular Functionmolecular function unknown
Biochemical PathwayDNA repair; biological process unknown
Regulatory Pathway
KEGG PathwaysK02346
Orthologs
Homologs GI Percent Identity Evalue Score
Homo sapienspolymerase (DNA directed) kappa [Homo sapiens]411e-41168
DEG Information
DEG Protein DEG Organism Percent Identity Evalue Bit Score
HP0760 conserved hypothetical proteinHelicobacter pylori27%0.07933.1
Post Translational Modification
PTM Type PTM Sub Type Score Modification Site Prosite ID
PDOC50173UmuC domain profile48.878145-327PS50173
AcylationN-myristoylation site43-48; 189-194; 280-285; 325-330; 368-373; 370-375; 492-497; PS00008
AmidationAmidation site198-201; PS00009
GlycosylationN-glycosylation site494-497; PS00001
PhosphorylationcAMP- and cGMP-dependent protein kinase phosphorylation site425-428; 584-587; PS00004
PhosphorylationCasein kinase II phosphorylation site12-15; 117-120; 130-133; 228-231; 257-260; 307-310; 391-394; 484-487; 495-498; PS00006
PhosphorylationProtein kinase C phosphorylation site98-100; 164-166; 376-378; 459-461; 495-497; 590-592; PS00005
DNA polymerase kappa, putative [Q4Q8C4]
Model Information
Template PDB ID2oh2B
Percent Identity41%
Target Region60-616
Template Region21-440
Domain Information
Domains Start End
Active Site Information
Residue Active Site Number Functional Part
Co-Factor
Metal Description
Ligands
CAS number Name Mol. Weight Mol. Formula Smile Notation PDB Reference
7791-18-6MAGNESIUM ION24.305Mg[Mg+2]2oh2
154-87-0THYMIDINE-5'-TRIPHOSPHATE482.168C10 H17 N2 O14 P3O=P(O)(O)OP(=O)(O)OP(=O)(O)OCC2OC(N1C(=O)NC(=O)C(=C1)C)CC2O2oh2
Mutational Information
Residue Feature Description
Modeled Protein Template Structure
+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/2093850/Q4Q8C4.pdb 2.0 539 = residues | | = | +| Ramachandran plot: 90.0% core 9.1% allow 0.8% gener 0.0% = disall | | = | +| All Ramachandrans: 15 labelled residues (out of 537) = | +| Chi1-chi2 plots: 6 labelled residues (out of 334) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 5.9 Bad contacts: = 8 | *| Bond len/angle: 5.9 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.08 Covalent: -0.22 Overall: = -0.03 | | = | | M/c bond lengths: 98.3% within limits 1.7% highlighted = | | M/c bond angles: 92.8% within limits 7.2% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further.
Overlapped Structure Procheck Summary
LeishBase: Leishmania Structural Database
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