| DEAD box RNA helicase, putative [Q4Q8B2] | |
|---|---|
| Systematic Name | LmjF.28.1530 [Leishmania major] |
| Gene Name | LMJF_28_1530 |
| Molecular Weight | 43998 Da |
| Protein Sequence Size | 389 |
| Function | |
| Charge | 2 |
| Isoelectric Point | 6.8516 pH |
| Description | DEAD box RNA helicase, putative. |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4Q8B2|Q4Q8B2_LEIMA DEAD box RNA helicase, putative - Leishmania major ETEQVDNIQANVLAIPTFEAMGLKEDLLKGMYSFGYKQPTAIQKRFIMPFLKGRDVIAQA SSGTGKTSAFCVCLLQACDPHTRETQALILSPTRELAVQTQDLCNNIGHHMGLTAYACIG GKSTEEDIRRLESGVHIVSGTPGRVFDMIRRKSLRVNGLKTLVLDEADEMLGKGFKAQIH DIYRMVPPLQIILVSATLPADVLEMTEKFMTEPTSILVKRDEITVDSVKQYFVSVDEEKN KFDVLMELYDSLTIAHAVVFCNTRKKVEQLAKKMTREKFAVSAMHGDMPQAERDEIMRQF RNGHSRVLITTDLWARGIDVERVSLVLSYDLPLAREQYIHRIGRTGRMGRTGLAITFVRH DELRLLRDIEQFYATQIEELPANIGEQM |
| DNA Sequence | >LmjF28.1530 |||DEAD box RNA helicase, putative|Leishmania major|chr 28|||Manual ATGGAGACGG AGCAAGTAGA TAATATTCAG GCCAATGTGC TTGCCATCCC AACCTTTGAGGCGATGGGGT TGAAGGAGGA CCTGCTCAAG GGCATGTACA GCTTCGGTTA CAAGCAGCCCACCGCCATTC AGAAGCGCTT CATTATGCCC TTCCTAAAGG GACGCGACGT GATTGCGCAGGCGTCTTCGG GTACGGGCAA GACGTCCGCC TTCTGCGTCT GTCTCCTGCA AGCGTGCGACCCGCACACGC GTGAGACGCA GGCCCTCATC CTCTCGCCGA CTCGCGAGCT GGCGGTGCAGACGCAGGATT TGTGCAACAA CATTGGCCAC CACATGGGGC TGACAGCGTA CGCGTGCATCGGTGGCAAGA GCACGGAGGA GGACATCCGC CGTCTGGAGA GCGGTGTGCA CATCGTCTCGGGCACGCCCG GCCGCGTCTT TGATATGATC CGCCGCAAGA GCCTTCGCGT AAATGGGCTGAAAACCCTCG TGCTGGACGA GGCTGATGAA ATGCTGGGCA AAGGCTTTAA GGCTCAGATTCACGACATCT ACCGCATGGT CCCACCGCTG CAGATCATCC TCGTGTCGGC GACGCTGCCGGCCGACGTGC TGGAGATGAC GGAGAAGTTC ATGACGGAGC CCACGAGCAT TCTCGTGAAGCGGGATGAGA TCACCGTGGA TAGCGTTAAA CAGTACTTTG TCTCCGTCGA CGAAGAGAAAAACAAATTCG ATGTCTTGAT GGAGTTGTAC GACAGCCTCA CGATCGCCCA CGCCGTGGTCTTCTGCAACA CCCGCAAAAA GGTGGAGCAG CTTGCCAAGA AGATGACGCG CGAGAAGTTCGCTGTGTCCG CCATGCACGG CGACATGCCC CAGGCGGAGC GCGACGAGAT TATGCGGCAGTTCCGTAATG GACACAGCCG CGTGCTCATT ACAACGGACC TGTGGGCGCG TGGCATTGATGTGGAGCGCG TCTCGCTCGT CCTCAGCTAC GATTTGCCGC TCGCGCGTGA GCAGTACATTCACCGCATCG GCCGCACTGG CCGCATGGGC CGCACTGGAC TGGCCATCAC CTTCGTGCGCCACGACGAGC TGCGCTTGTT GCGCGACATT GAGCAGTTCT ACGCGACGCA AATTGAAGAACTCCCCGCGA ATATCGGCGA GCAAATGTAG |
| DEAD box RNA helicase, putative Q4Q8B2] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; ATP-dependent helicase activity; helicase activity; nucleic acid binding |
| Biochemical Pathway | nucleobase, nucleoside, nucleotide and nucleic acid metabolism |
| Regulatory Pathway | |
| KEGG Pathways | K03257 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 [Homo sapiens] | 58 | 1e-129 | 458 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YDR021w involved in maturation of 18S rRNA | Saccharomyces cerevisiae | 51% | 1e-110 | 393 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00017 | ATP/GTP-binding site motif A (P-loop) | 61-68; | PS00017 | |
| PDOC00039 | DEAD and DEAH box families ATP-dependent helicases signatures | 164-172; | PS00039 | |
| PDOC51195 | DEAD-box RNA helicase Q motif profile | 10.265 | 17-45 | PS51195 |
| PDOC51192 | Superfamilies 1 and 2 helicase domain profiles | 26.636 | 48-217 | PS51192 |
| PDOC51192 | Superfamilies 1 and 2 helicase domain profiles | 24.471 | 228-389 | PS51194 |
| Acylation | N-myristoylation site | 64-69; 121-126; 353-358; | PS00008 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 151-154; 273-276; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 124-127; 125-128; 235-238; 376-379; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 65-67; 154-156; 207-209; 228-230; 264-266; 346-348; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 368-374; | PS00007 | |
| DEAD box RNA helicase, putative [Q4Q8B2] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2j0uB | |
| Percent Identity | 58% | |
| Target Region | 17-391 | |
| Template Region | 38-347 | |
| Domain Information | ||
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| Domains | Start | End |
| Active Site Information | ||
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| Residue | Active Site Number | Functional Part |
| Co-Factor | |
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| Metal | Description |
| Ligands | |||||
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| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| Mutational Information | ||
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| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/893550/Q4Q8B2.pdb 2.0 373 = residues | | = | *| Ramachandran plot: 90.4% core 7.5% allow 1.5% gener 0.6% = disall | | = | *| All Ramachandrans: 19 labelled residues (out of 371) = | +| Chi1-chi2 plots: 4 labelled residues (out of 235) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 5.3 Bad contacts: = 7 | *| Bond len/angle: 13.7 Morris et al class: 1 = 1 3 | +| 3 cis-peptides = | | G-factors Dihedrals: -0.07 Covalent: -0.40 Overall: = -0.19 | | = | *| M/c bond lengths: 98.5% within limits 1.5% highlighted 3 off = graph | *| M/c bond angles: 91.7% within limits 8.3% highlighted 8 off = graph | +| Planar groups: 99.3% within limits 0.7% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |