| Serine hydroxymethyltransferase [Q4Q828] | |
|---|---|
| Systematic Name | LmjF.28.2370 [Leishmania major] |
| Gene Name | SHMT-L |
| Molecular Weight | 52413 Da |
| Protein Sequence Size | 474 |
| Function | |
| Charge | 12 |
| Isoelectric Point | 8.7911 pH |
| Description | Serine hydroxymethyltransferase (EC 2.1.2.1). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 2.1.2.1 |
| Sequence | >tr|Q4Q828|Q4Q828_LEIMA Serine hydroxymethyltransferase (EC 2.1.2.1) - Leishmania major ILRAAPASTHRHRSPSLPGNISLRDHDPEVHQLIQREMRRQIEGLELIASENFTSRAVLD CLGSVLTNKYAEGLPGNRYYGGTEVVDELENLCVRRARAAFCLDAALWGVSVQPYSGSPA NLAVYTALLRPHDRMMGLSLQAGGHLTHGFYTATKRLSASSIFFESLPYSITPKGLVDYD QLAYLADIYKPRLIIAGGSAYPRDWDYKRYREICDSVGAYFMVDMSHFSGLVAAREHNDP FEYADVVTTTTHKTLRGPRSGMIFFKKSIKQGKENVCVEDSINNAVFPALQGGPHLHQIA GIATQLKEVASPEWRTYIKQVKANARALAAVLTEGGETLVSGGTDNHLLLWNLRPHGLTG SKLEKLLDMVNITVNKNTIFGDRSAQAPYGIRLGTPALTTRGLQEEDFRRVGQLLIRSVQ LSKEVQKSAGSTKLVDFVKAAETSKALQEMAEEVKAYARQFPYPGLESAYPIQ |
| DNA Sequence | >LmjF28.2370 |SHMT||serine hydroxymethyltransferase|Leishmania major|chr 28|||Manual ATGATCCTGC GGGCGGCCCC CGCCTCCACG CACCGTCACC GAAGTCCATC TCTGCCCGGCAACATATCTC TCAGAGACCA TGATCCTGAG GTGCACCAGC TTATTCAGAG AGAGATGCGCCGGCAGATAG AAGGGCTAGA GCTGATTGCG TCGGAGAACT TTACCTCCCG CGCGGTGCTTGACTGCCTCG GGTCTGTCCT CACGAACAAG TACGCAGAGG GTCTGCCTGG GAACCGCTACTACGGCGGCA CGGAGGTCGT TGACGAGCTC GAGAACCTGT GCGTGCGGCG CGCACGCGCAGCATTTTGCT TGGATGCTGC CTTATGGGGC GTCAGCGTGC AGCCGTACAG CGGCTCACCTGCCAACCTGG CCGTCTACAC CGCCCTGTTG CGCCCGCATG ACCGCATGAT GGGCCTCTCACTGCAGGCGG GCGGCCACCT CACGCACGGC TTCTACACCG CAACGAAGCG CCTCTCGGCCTCCTCGATCT TCTTCGAAAG CCTCCCGTAC TCCATCACGC CGAAGGGCCT CGTCGACTACGATCAGCTAG CGTACTTGGC GGATATCTAC AAGCCACGGC TCATCATTGC CGGCGGCTCCGCCTACCCGC GCGACTGGGA CTACAAGCGC TACCGCGAAA TCTGCGACAG CGTTGGTGCCTACTTCATGG TGGACATGTC CCACTTTTCT GGCCTGGTTG CCGCCCGGGA GCACAACGACCCGTTTGAGT ACGCCGATGT CGTGACCACC ACCACCCACA AGACGCTGCG CGGGCCTCGGTCTGGCATGA TCTTCTTCAA GAAGTCGATA AAGCAGGGCA AGGAGAACGT GTGCGTGGAGGATTCCATCA ACAACGCCGT CTTCCCCGCG CTACAGGGTG GTCCACACCT CCACCAGATCGCCGGCATCG CCACACAGCT GAAGGAAGTG GCATCGCCGG AGTGGCGCAC CTACATAAAGCAAGTGAAGG CGAACGCCAG GGCCCTGGCC GCCGTCCTCA CGGAGGGTGG GGAGACGCTCGTCAGCGGCG GCACCGACAA CCACCTGCTT CTGTGGAACC TCCGCCCGCA CGGCCTCACAGGCTCCAAGC TGGAGAAGCT GCTCGACATG GTCAACATCA CGGTGAACAA GAACACTATCTTTGGCGACA GAAGCGCCCA GGCGCCGTAC GGCATACGTC TTGGCACACC AGCCCTCACCACCCGCGGCC TCCAGGAGGA GGACTTCAGG CGGGTCGGCC AGCTCCTCAT TCGCTCGGTGCAGCTCTCCA AGGAGGTGCA GAAGTCCGCC GGCTCTACGA AGCTCGTCGA CTTCGTGAAGGCCGCCGAGA CCTCGAAGGC GTTGCAGGAG ATGGCCGAGG AGGTGAAGGC GTATGCGCGTCAGTTCCCGT ACCCCGGCCT GGAGAGCGCC TATCCCATTC AGTAG |
| Serine hydroxymethyltransferase Q4Q828] | |
|---|---|
| Metabolite Information | |
| Molecular Function | glycine hydroxymethyltransferase activity |
| Biochemical Pathway | L-serine metabolism; glycine metabolism |
| Regulatory Pathway | |
| KEGG Pathways | K00600 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | serine hydroxymethyltransferase 2 (mitochondrial) [Homo sapiens] | 58 | 1e-145 | 511 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| glyA Serine hydroxymethyltransferase (EC 2.1.2.1) | Escherichia coli MG1655 | 47% | 2e-93 | 337 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00090 | Serine hydroxymethyltransferase pyridoxal-phosphate attachment site | 246-262; | PS00096 | |
| PDOC00574 | Trp-Asp (WD-40) repeats signature and profiles | 340-354; | PS00678 | |
| Acylation | N-myristoylation site | 64-69; 74-79; 118-123; 144-149; 231-236; 343-348; 358-363; 391-396; 466-471; | PS00008 | |
| Glycosylation | N-glycosylation site | 21-24; 53-56; 372-375; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 156-159; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 23-26; 362-365; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 10-12; 23-25; 55-57; 68-70; 155-157; 173-175; 252-254; 255-257; 269-271; 400-402; 432-434; 444-446; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 204-211; 236-244; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 237-251; | PS00003 | |
| Serine hydroxymethyltransferase [Q4Q828] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2a7vA | |
| Percent Identity | 58% | |
| Target Region | 17-480 | |
| Template Region | 26-420 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| GLU | 57 | Sidechain |
| THR | 235 | Sidechain |
| LYS | 238 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Pyridoxal phosphate | |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/663728/Q4Q828.pdb 2.0 458 = residues | | = | *| Ramachandran plot: 91.7% core 7.0% allow 0.8% gener 0.5% = disall | | = | +| All Ramachandrans: 8 labelled residues (out of 456) = | +| Chi1-chi2 plots: 1 labelled residues (out of 265) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.1 Bad contacts: = 4 | *| Bond len/angle: 6.8 Morris et al class: 1 = 1 2 | +| 3 cis-peptides = | | G-factors Dihedrals: 0.07 Covalent: -0.21 Overall: = -0.03 | | = | | M/c bond lengths: 99.0% within limits 1.0% highlighted = | | M/c bond angles: 93.6% within limits 6.4% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |