| Vacuolar ATP synthase subunit B, putative [Q4Q821] | |
|---|---|
| Systematic Name | LmjF.28.2430 [Leishmania major] |
| Gene Name | LMJF_28_2430 |
| Molecular Weight | 55519 Da |
| Protein Sequence Size | 495 |
| Function | Noncatalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells |
| Charge | -6 |
| Isoelectric Point | 5.5451 pH |
| Description | Vacuolar ATP synthase subunit B, putative (EC 3.6.3.14). |
| Subcellular Location | cytoplasm; lysosomal hydrogen-translocating V-type ATPase complex; proton-transporting two-sector ATPase complex[Predict] |
| E. C. Number | 3.6.3.14 |
| Sequence | >tr|Q4Q821|Q4Q821_LEIMA Vacuolar ATP synthase subunit B, putative (EC 3.6.3.14) - Leishmania major GRDEEHVRVLSKQELLATHIKELNESYSVKPHLEYTTIRAVNGPLVILEDVRKPTFAEIV NIELADGTARRGQVLEVDGTKAVVQVFEGTSGIDVVRSKCEFTGKVMELGVSEDMLGRIF NGSGIPIDNGPPVLPEQFRSIEGIPINPRARVYPEEMIQTGISSIDVMTSISRGQKIPLF SGAGLPHNEIAAQIVRQAGLVKREGKTEDFCVVFAAMGVNQETARFFRTEFEQNGSMEKT VLFLNLANDPTIERIVTPRLALTTAEYLAYDCGKHVLVILTDMSSYADALREVSAAREEV PGRRGFPGYMYTNLACIYERAGRVLGRAGSITQIPILSMPNDDITHPIPDLTGYITEGQI YVDRQLHNRQLYPPINILPSLSRLMKNAIGEGMTRKDHSGVSNQMYAAYAISRDILAMKA VVGEEALSSEDLLYLEFLDKFEHKFICQGFYETRDIFQSLDLCWELLRTFPKSMLNKIDM KTRDEFYDRHPGRK |
| DNA Sequence | >LmjF28.2430 |||vacuolar ATP synthase subunit B, putative|Leishmania major|chr 28|||Manual ATGGGCCGCG ATGAGGAACA TGTTCGCGTG CTGTCGAAGC AGGAGCTGCT CGCCACGCACATCAAGGAGC TCAACGAGAG CTACTCGGTG AAACCGCATC TTGAGTACAC GACGATTCGTGCCGTCAACG GTCCCCTCGT CATCCTGGAG GATGTGCGCA AGCCGACCTT CGCCGAGATCGTCAACATCG AGCTGGCCGA CGGCACCGCG CGTCGTGGCC AGGTGCTCGA GGTGGATGGCACCAAGGCCG TCGTGCAGGT CTTCGAGGGC ACCTCCGGTA TCGATGTCGT GCGCTCCAAGTGCGAGTTTA CCGGCAAGGT TATGGAGCTC GGCGTAAGCG AGGACATGCT GGGCCGCATCTTCAACGGTT CCGGCATCCC GATCGATAAC GGCCCACCGG TGCTGCCGGA GCAGTTCCGCAGTATCGAGG GTATTCCAAT CAACCCGCGT GCGCGTGTCT ACCCGGAGGA GATGATCCAGACGGGTATCT CGTCCATCGA CGTCATGACC TCCATCTCGC GCGGCCAGAA GATTCCGCTCTTCTCCGGCG CTGGGCTGCC GCACAATGAA ATCGCCGCGC AGATTGTGCG TCAGGCCGGTCTCGTGAAGC GCGAGGGCAA GACGGAGGAC TTCTGTGTCG TCTTCGCCGC CATGGGTGTGAACCAGGAGA CGGCCCGCTT TTTCCGCACA GAGTTCGAGC AGAACGGCTC GATGGAGAAGACCGTCCTCT TCCTGAACCT GGCGAACGAC CCGACCATTG AGCGCATCGT GACGCCGCGTCTGGCCCTCA CTACTGCTGA GTACCTCGCC TACGACTGCG GTAAGCACGT GCTCGTCATTCTGACCGACA TGTCCTCGTA CGCCGATGCC CTGCGTGAGG TGTCCGCCGC CCGTGAGGAGGTGCCCGGCC GCCGAGGTTT CCCTGGCTAC ATGTACACGA ATCTGGCGTG CATCTACGAGCGTGCAGGCC GTGTGCTGGG CCGCGCCGGC TCCATCACCC AGATCCCGAT TCTGTCCATGCCGAACGATG ATATCACCCA CCCCATTCCA GATCTCACCG GGTACATTAC AGAAGGCCAGATCTACGTGG ACCGCCAGCT GCACAACCGG CAGCTGTACC CGCCGATTAA TATTCTGCCGTCGCTGTCGC GTCTGATGAA GAACGCCATT GGTGAGGGCA TGACCCGCAA GGATCACAGCGGCGTGAGCA ACCAAATGTA CGCCGCCTAC GCCATCAGTC GTGATATTCT CGCCATGAAGGCCGTCGTTG GCGAGGAGGC GTTGAGTAGC GAGGATCTGC TGTACCTCGA GTTTCTCGACAAGTTCGAGC ACAAGTTCAT CTGCCAAGGC TTCTACGAAA CACGCGATAT CTTCCAAAGCCTTGACCTGT GCTGGGAACT GCTGCGCACG TTCCCCAAGA GCATGCTGAA CAAGATCGACATGAAGACCC GTGATGAGTT TTATGACCGC CACCCGGGCC GCAAGTAA |
| Vacuolar ATP synthase subunit B, putative Q4Q821] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; hydrogen-exporting ATPase activity, phosphorylative mechanism; hydrogen-transporting ATP synthase activity, rotational mechanism; hydrogen-transporting ATPase activity, rotational mechanism |
| Biochemical Pathway | ATP biosynthesis; ATP hydrolysis coupled proton transport; ATP synthesis coupled proton transport; energy coupled proton transport, against electrochemical gradient |
| Regulatory Pathway | |
| KEGG Pathways | K02147 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | ATPase, H+ transporting, lysosomal 56/58kD, V1 subunit B, isoform 2 [Homo sapiens] | 73 | 0 | 695 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| HP1132 ATP synthase F1, subunit beta (atpD) | Helicobacter pylori | 27% | 1e-32 | 135 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00137 | ATP synthase alpha and beta subunits signature | 373-382; | PS00152 | |
| Acylation | N-myristoylation site | 184-189; | PS00008 | |
| Amidation | Amidation site | 301-304; 491-494; | PS00009 | |
| Glycosylation | N-glycosylation site | 25-28; 121-124; 234-237; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 53-56; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 12-15; 56-59; 91-94; 98-101; 163-166; 229-232; 263-266; 285-288; 394-397; 428-431; 482-485; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 29-31; 38-40; 68-70; 103-105; 223-225; 257-259; 394-396; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 481-487; | PS00007 | |
| Vacuolar ATP synthase subunit B, putative [Q4Q821] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2c61B | |
| Percent Identity | 56% | |
| Target Region | 44-495 | |
| Template Region | 13-425 | |
| Domain Information | ||
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| Domains | Start | End |
| Active Site Information | ||
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| Residue | Active Site Number | Functional Part |
| ARG | 341 | Unknown |
| Co-Factor | |
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| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| Mutational Information | ||
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| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/2309040/Q4Q821.pdb 2.0 452 = residues | | = | *| Ramachandran plot: 91.0% core 6.6% allow 1.8% gener 0.5% = disall | | = | +| All Ramachandrans: 13 labelled residues (out of 450) = | +| Chi1-chi2 plots: 7 labelled residues (out of 275) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.9 Bad contacts: = 11 | *| Bond len/angle: 18.3 Morris et al class: 1 = 1 2 | +| 2 cis-peptides = | +| G-factors Dihedrals: -0.01 Covalent: -0.52 Overall: = -0.20 | | = | *| M/c bond lengths: 98.0% within limits 2.0% highlighted 10 off = graph | *| M/c bond angles: 92.5% within limits 7.5% highlighted 7 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |