| Cytosolic malate dehydrogenase, putative [Q4Q7X6] | |
|---|---|
| Systematic Name | LmjF.28.2860 [Leishmania major] |
| Gene Name | cMDH |
| Molecular Weight | 34155 Da |
| Protein Sequence Size | 324 |
| Function | |
| Charge | -1.5 |
| Isoelectric Point | 6.1673 pH |
| Description | Cytosolic malate dehydrogenase, putative (EC 1.1.1.37). |
| Subcellular Location | cytoplasm[Predict] |
| E. C. Number | 1.1.1.37 |
| Sequence | >tr|Q4Q7X6|Q4Q7X6_LEIMA Cytosolic malate dehydrogenase, putative (EC 1.1.1.37) - Leishmania major SAVKVAVTGAAGQIGYALVPLIARGALLGPTTPVELRLLDIEPALKALAGVEAELEDCAF PLLDKVVVTADPRVAFDGVAIAIMCGAFPRKAGMERKDLLEMNARIFKEQGEAIAAVAAS DCRVVVVGNPANTNALILLKSAQGKLNPRHVTAMTRLDHNRALSLLARKAGVPVSQVRNV IIWGNHSSTQVPDTDSAVIGTTPAREAIKDDALDDDFVQVVRGRGAEIIQLRGLSSAMSA AKAAVDHVHDWIHGTPEGVYVSMGVYSDENPYGVPSGLIFSFPCTCHAGEWTVVSGKLNG DLGKQRLASTIAELQEERAQAGL |
| DNA Sequence | >LmjF28.2860 |||cytosolic malate dehydrogenase, putative|Leishmania major|chr 28|||Manual ATGTCCGCTG TGAAGGTGGC CGTGACGGGT GCGGCTGGTC AGATCGGGTA TGCATTGGTACCGCTGATCG CGCGCGGCGC CCTCCTCGGG CCCACCACGC CTGTGGAGCT CCGCCTGCTGGATATCGAGC CCGCTCTGAA GGCGCTTGCC GGCGTCGAAG CGGAGCTGGA AGACTGCGCCTTCCCTCTCC TGGACAAGGT CGTCGTCACG GCAGACCCGC GCGTTGCCTT CGACGGGGTCGCCATTGCCA TCATGTGCGG TGCCTTCCCG CGCAAGGCAG GGATGGAGCG CAAGGACCTCCTCGAGATGA ACGCGCGCAT CTTCAAGGAG CAGGGTGAGG CCATCGCTGC TGTGGCCGCGTCAGACTGCC GCGTGGTGGT TGTCGGCAAT CCGGCCAACA CAAACGCTCT CATCCTCCTCAAGTCAGCTC AGGGGAAGTT GAACCCCCGT CATGTGACCG CCATGACCCG CCTCGATCACAACCGTGCCT TGTCTCTTCT GGCTCGCAAG GCCGGTGTGC CGGTGTCGCA GGTGCGCAACGTGATCATCT GGGGCAACCA TAGCTCTACA CAGGTCCCTG ACACTGACAG CGCTGTGATCGGCACCACTC CGGCCCGCGA AGCGATCAAG GACGATGCAC TCGATGACGA CTTTGTGCAGGTGGTGCGCG GGCGCGGCGC CGAAATCATC CAACTACGTG GTCTTTCCTC AGCCATGTCCGCCGCCAAGG CCGCCGTAGA CCACGTACAT GACTGGATAC ATGGCACACC GGAGGGCGTGTACGTTTCGA TGGGCGTGTA CTCGGATGAA AACCCGTACG GCGTGCCGAG CGGCCTCATCTTCTCCTTTC CGTGCACGTG CCACGCCGGC GAGTGGACTG TTGTCTCGGG CAAGCTGAATGGGGACCTCG GGAAGCAGCG TCTCGCCAGC ACGATCGCCG AGCTGCAGGA GGAGAGGGCACAGGCAGGGC TATGA |
| Cytosolic malate dehydrogenase, putative Q4Q7X6] | |
|---|---|
| Metabolite Information | |
| Molecular Function | malate dehydrogenase activity; oxidoreductase activity |
| Biochemical Pathway | malate metabolism; tricarboxylic acid cycle; tricarboxylic acid cycle intermediate metabolism |
| Regulatory Pathway | |
| KEGG Pathways | K00026 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | cytosolic malate dehydrogenase [Homo sapiens] | 49 | 4e-77 | 285 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| mdh malate dehydrogenase | Haemophilus influenzae | 28% | 0.000000000000004 | 76.3 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00676 | Carbamoyl-phosphate synthase subdomain signatures | 100-107; | PS00867 | |
| PDOC00066 | Malate dehydrogenase active site signature | 155-167; | PS00068 | |
| Acylation | N-myristoylation site | 79-84; 129-134; 172-177; 185-190; 201-206; 234-239; 255-260; 259-264; 274-279; 278-283; | PS00008 | |
| Glycosylation | N-glycosylation site | 186-189; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 33-36; 156-159; 311-314; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 296-298; | PS00005 | |
| Cytosolic malate dehydrogenase, putative [Q4Q7X6] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2cvqB | |
| Percent Identity | 53% | |
| Target Region | 1-324 | |
| Template Region | 1-327 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 159 | Sidechain |
| ARG | 161 | Sidechain |
| HIS | 187 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | 743.405 | C21 H28 N7 O17 P3 | c1cc(c[n+](c1)C2C(C(C(O2)COP(=O)([O-])OP(=O)(O)OCC3C(C(C(O3)n4cnc5c4ncnc5N)OP(=O)(O)O)O)O)O)C(=O)N | 2cvq | |
| 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | 122.143 | C4 H12 N O3 | OCC([NH3+])(CO)CO | 2cvq | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/4859484/Q4Q7X6.pdb 2.0 324 = residues | | = | | Ramachandran plot: 94.2% core 5.8% allow 0.0% gener 0.0% = disall | | = | +| All Ramachandrans: 6 labelled residues (out of 322) = | | Chi1-chi2 plots: 0 labelled residues (out of 163) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | | Residue properties: Max.deviation: 2.7 Bad contacts: = 4 | +| Bond len/angle: 4.7 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.13 Covalent: -0.08 Overall: = 0.05 | | = | | M/c bond lengths: 99.7% within limits 0.3% highlighted = | | M/c bond angles: 95.2% within limits 4.8% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |