| Mitogen-activated protein kinase, putative [Q4Q7S2] | |
|---|---|
| Systematic Name | LmjF.30.0370 [Leishmania major] |
| Gene Name | MPK12 |
| Molecular Weight | 46332 Da |
| Protein Sequence Size | 410 |
| Function | |
| Charge | -4 |
| Isoelectric Point | 5.9 pH |
| Description | Mitogen-activated protein kinase, putative (Protein kinase, putative) (EC 2.7.1.-). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 2.7.1.- |
| Sequence | >tr|Q4Q7S2|Q4Q7S2_LEIMA Mitogen-activated protein kinase, putative (Protein kinase, putative) (EC 2.7.1.-) - Leishmania major. ILTKVEGPNAAGNKVYVFGVNDYRLEVPERYNVQHFVGRGAYGFVCSAVDAVTNEPVAIK KVMHLFDDAVDAKRVLREVKLLAYLKHPNILSLKDLFKSPDPVDTYSELYVVTDLMESDM DAILRSPRIRLAAGHGQYFTLQLLCALQYIHSAHVLHRDLKPGNLLTDSECNLKLGDFGL ARGIGHDDTMTQYVFTRWYRPPELLLVCKHCNYSADMWAVGCLAAEMFTGKPLFPGKDYI NQINLIVELLGIPDLARDLPPSTSKEAIHYLSSLPPSKGKKLEEYAPELRRRFDETTFYD SFDTELEEAIAVEGAIIARPRPHPPEEYYAEFVDFIFGLLCYNPAKRRTAKESIAHAWLS DVRGPQETIGGCEAERIYRWDADGTAFTIPQLRQLFIDEIGKFASTRSS |
| DNA Sequence | >LmjF30.0370 |MPK12||mitogen-activated protein kinase, putative|Leishmania major|chr 30|||Manual ATGATACTCA CCAAGGTAGA GGGCCCTAAT GCGGCTGGCA ACAAGGTTTA CGTCTTTGGTGTGAACGACT ACCGCCTTGA GGTGCCAGAG CGCTACAACG TTCAGCACTT CGTTGGGCGTGGCGCTTACG GATTTGTGTG CAGCGCCGTG GACGCGGTGA CGAACGAGCC GGTTGCGATTAAGAAGGTGA TGCACCTCTT CGACGATGCC GTCGATGCAA AGCGCGTCTT GCGGGAGGTGAAGCTGCTAG CCTATCTCAA ACACCCAAAC ATCCTCTCTC TCAAGGACCT CTTCAAGTCACCGGACCCCG TTGACACGTA CAGCGAGCTC TACGTCGTGA CTGACTTGAT GGAGTCGGACATGGACGCCA TCCTGCGCTC CCCACGCATC CGTCTCGCCG CCGGGCACGG GCAGTACTTCACCCTTCAGC TGCTGTGCGC ACTGCAATAC ATTCACAGTG CGCATGTGCT GCACCGAGACCTGAAGCCGG GGAACCTGCT GACAGATTCG GAGTGCAATC TTAAGCTGGG CGACTTTGGCCTCGCGCGCG GTATCGGGCA TGACGACACC ATGACACAGT ACGTGTTCAC GCGGTGGTACCGGCCACCAG AGTTGCTGCT GGTGTGCAAG CACTGCAACT ACAGCGCGGA CATGTGGGCTGTTGGCTGCC TTGCGGCAGA GATGTTCACC GGCAAGCCGC TCTTTCCGGG CAAGGACTACATCAACCAGA TCAATCTCAT AGTGGAGTTG CTGGGCATAC CAGACCTGGC ACGTGACCTGCCGCCGAGCA CGTCAAAGGA GGCGATACAC TACCTCTCCT CCCTGCCCCC TAGCAAGGGGAAGAAGCTTG AGGAGTACGC CCCGGAGCTG CGACGTCGCT TCGACGAGAC CACCTTTTATGACAGCTTCG ACACGGAGCT AGAGGAGGCG ATCGCTGTGG AAGGCGCCAT CATTGCCCGCCCTCGGCCAC ACCCGCCGGA GGAGTACTAC GCTGAGTTTG TCGACTTCAT CTTTGGACTGCTTTGCTACA ACCCCGCGAA GCGGCGCACG GCGAAAGAGT CCATAGCGCA TGCGTGGCTGTCGGACGTGC GCGGCCCACA GGAGACCATT GGCGGCTGCG AGGCGGAGCG AATATACCGTTGGGATGCCG ACGGCACCGC CTTCACCATC CCACAACTTC GCCAGCTGTT CATAGATGAGATCGGAAAGT TTGCGTCTAC CCGGAGCAGC TGA |
| Mitogen-activated protein kinase, putative Q4Q7S2] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; protein kinase activity; protein serine/threonine kinase activity; protein-tyrosine kinase activity |
| Biochemical Pathway | protein amino acid phosphorylation |
| Regulatory Pathway | |
| KEGG Pathways | |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | mitogen-activated protein kinase 7 isoform 1 [Homo sapiens] | 43 | 2e-59 | 227 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YDL108w cyclin-dependent serthr protein kinase | Saccharomyces cerevisiae | 29% | 2e-36 | 147 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC01049 | MAP kinase signature | 67-172; | PS01351 | |
| PDOC00100 | Protein kinases signatures and profile | 38-62; | PS00107 | |
| PDOC00100 | Protein kinases signatures and profile | 156-168; | PS00108 | |
| PDOC00100 | Protein kinases signatures and profile | 40.725 | 32-360 | PS50011 |
| Acylation | N-myristoylation site | 137-142; 164-169; 180-185; 222-227; 315-320; 371-376; 385-390; | PS00008 | |
| Amidation | Amidation site | 279-282; | PS00009 | |
| Glycosylation | N-glycosylation site | 213-216; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 347-350; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 4-7; 48-51; 93-96; 106-109; 119-122; 168-171; 264-267; 298-301; 305-308; 350-353; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 93-95; 127-129; 230-232; 264-266; 350-352; 406-408; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 292-300; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 100-114; 293-307; | PS00003 | |
| Mitogen-activated protein kinase, putative [Q4Q7S2] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2ozaB | |
| Percent Identity | 45% | |
| Target Region | 14-410 | |
| Template Region | 6-340 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 147 | Sidechain |
| LYS | 149 | Sidechain |
| ASN | 152 | Sidechain |
| THR | 184 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/422320/Q4Q7S2.pdb 2.0 397 = residues | | = | *| Ramachandran plot: 88.2% core 10.9% allow 0.6% gener 0.3% = disall | | = | *| All Ramachandrans: 18 labelled residues (out of 395) = | +| Chi1-chi2 plots: 2 labelled residues (out of 246) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 3 | *| Bond len/angle: 5.4 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: -0.02 Covalent: -0.20 Overall: = -0.08 | | = | | M/c bond lengths: 98.9% within limits 1.1% highlighted = | | M/c bond angles: 93.3% within limits 6.7% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |