| Glyceraldehyde 3-phosphate dehydrogenase, glycosomal [Q4Q6Z5] | |
|---|---|
| Systematic Name | LmjF.30.2970 [Leishmania major] |
| Gene Name | LMJF_30_2970 |
| Molecular Weight | 39167 Da |
| Protein Sequence Size | 361 |
| Function | |
| Charge | 11.5 |
| Isoelectric Point | 9.3372 pH |
| Description | Glyceraldehyde 3-phosphate dehydrogenase, glycosomal (EC 1.2.1.12). |
| Subcellular Location | glycosome[Predict] |
| E. C. Number | 1.2.1.12 |
| Sequence | >tr|Q4Q6Z5|Q4Q6Z5_LEIMA Glyceraldehyde 3-phosphate dehydrogenase, glycosomal (EC 1.2.1.12) - Leishmania major APIKVGINGFGRIGRMVLQAICDQGLIGNEIDVVAVVDMSTNAEYFAYQMKYDTVHGRPK YTVEAVKSTPSVKTPDVLVVNGHRIKCVKAQRNPADLPWGKLGVDYVIESTGLFTDKLQA EGHIKGGAKKVVISAPASGGAKTIVMGVNQHEYSPTSHHVVSNASCTTNCLAPIVHVLTK ENFGIETGLMTTIHSYTATQKTVDGVSLKDWRGGRAAAINIIPSTTGAAKAVGMVIPSTK GKLTGMSFRVPTPDVSVVDLTFRSTRETSIQEIDKAIKKAAQTYMKDILGFTNDELVSSD FINDNRSSVYDSKATLQNNLPGEKRFFKIVSWYDNEWGYSHRVVDLVRYMAATDAASAKM |
| DNA Sequence | >LmjF30.2970 |||glyceraldehyde 3-phosphate dehydrogenase, glycosomal|Leishmania major|chr 30|||Manual ATGGCTCCTA TCAAGGTCGG CATCAACGGC TTCGGCCGCA TTGGTCGCAT GGTGCTTCAGGCCATCTGTG ACCAGGGTCT TATCGGCAAC GAGATCGACG TCGTCGCTGT CGTGGACATGAGCACGAATG CTGAATACTT CGCGTACCAG ATGAAGTACG ACACGGTGCA CGGTCGCCCGAAGTACACGG TGGAGGCTGT GAAGAGCACC CCGTCCGTGA AGACGCCAGA TGTGCTTGTGGTGAACGGCC ACCGCATCAA GTGCGTGAAG GCGCAGCGCA ACCCCGCGGA TCTGCCGTGGGGCAAGCTCG GCGTGGATTA CGTGATCGAG TCTACTGGCC TGTTCACGGA CAAGTTGCAGGCCGAGGGTC ACATCAAGGG TGGTGCGAAG AAGGTCGTGA TCAGCGCGCC GGCGTCTGGCGGCGCCAAGA CGATCGTGAT GGGCGTGAAC CAGCACGAGT ACTCGCCGAC GTCGCACCACGTGGTGTCGA ACGCGTCGTG CACAACCAAC TGCCTGGCCC CCATCGTGCA TGTGCTGACAAAGGAGAACT TCGGCATCGA GACCGGTCTG ATGACCACCA TCCACTCCTA CACGGCGACGCAGAAGACGG TGGACGGCGT GTCGCTGAAG GACTGGCGCG GTGGCCGCGC GGCGGCCATAAACATCATCC CGAGCACGAC CGGCGCCGCC AAGGCCGTGG GCATGGTGAT CCCGTCGACCAAGGGCAAGC TGACCGGCAT GTCCTTCCGC GTGCCGACGC CGGACGTGTC CGTCGTGGACCTGACGTTCC GCTCGACGCG CGAAACGTCG ATCCAGGAGA TCGACAAGGC CATCAAGAAGGCCGCGCAGA CGTACATGAA GGACATTCTC GGCTTCACCA ACGACGAGCT CGTCAGCTCTGACTTTATTA ACGACAACCG CAGCTCGGTC TACGACTCCA AGGCGACACT GCAGAACAACCTACCCGGCG AGAAGCGGTT CTTCAAGATC GTCTCGTGGT ATGACAACGA GTGGGGCTACTCGCACCGCG TGGTGGACCT GGTGCGCTAC ATGGCCGCCA CGGACGCTGC GAGCGCCAAGATGTAG |
| Glyceraldehyde 3-phosphate dehydrogenase, glycosomal Q4Q6Z5] | |
|---|---|
| Metabolite Information | |
| Molecular Function | NAD binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; glyceraldehyde-3-phosphate dehydrogenase activity |
| Biochemical Pathway | glucose metabolism; glycolysis |
| Regulatory Pathway | |
| KEGG Pathways | K00134 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | glyceraldehyde-3-phosphate dehydrogenase, spermatogenic [Homo sapiens] | 53 | 4e-95 | 345 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| gapA Glyceraldehyde 3-phosphate dehydrogenase A (EC 1.2.1.12) | Escherichia coli MG1655 | 51% | 2e-91 | 330 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 164-167; 306-309; | PS00722 | |
| PDOC00069 | Glyceraldehyde 3-phosphate dehydrogenase active site | 165-172; | PS00071 | |
| PDOC00299 | Microbodies C-terminal targeting signal | 359-361; | PS00342 | |
| Acylation | N-myristoylation site | 26-31; 140-145; 185-190; 189-194; 214-219; 228-233; | PS00008 | |
| Phosphorylation | Casein kinase II phosphorylation site | 42-45; 208-211; 257-260; 265-268; 270-273; 293-296; 309-312; 332-335; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 72-74; 116-118; 200-202; 208-210; 239-241; 248-250; 262-264; 265-267; 341-343; 358-360; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 343-350; | PS00007 | |
| Glyceraldehyde 3-phosphate dehydrogenase, glycosomal [Q4Q6Z5] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1i33F | |
| Percent Identity | 94% | |
| Target Region | 1-361 | |
| Template Region | 1-358 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| CYS | 167 | Sidechain |
| HIS | 195 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| N-1,2,3,4-TETRAHYDRONAPHTH-1-YL-2'-[3,5-DIMETHOXYBENZAMIDO]- 2'-DEOXY-ADENOSINE | 560.601 | C29 H32 N6 O6 | O=C(c1cc(OC)cc(OC)c1)NC6C(O)C(OC6n3cnc2c(ncnc23)NC5c4ccccc4CCC5)CO | 1i33 | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/527722/Q4Q6Z5.pdb 2.0 361 = residues | | = | *| Ramachandran plot: 92.1% core 6.6% allow 0.6% gener 0.6% = disall | | = | +| All Ramachandrans: 9 labelled residues (out of 359) = | +| Chi1-chi2 plots: 1 labelled residues (out of 189) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 2 | *| Bond len/angle: 5.5 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.08 Covalent: -0.06 Overall: = 0.02 | | = | | M/c bond lengths: 99.4% within limits 0.6% highlighted = | | M/c bond angles: 95.7% within limits 4.3% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |