| DNA ligase I, putative [Q4Q6U5] | |
|---|---|
| Systematic Name | LmjF.30.3440 [Leishmania major] |
| Gene Name | LMJF_30_3440 |
| Molecular Weight | 75433 Da |
| Protein Sequence Size | 681 |
| Function | |
| Charge | -4.5 |
| Isoelectric Point | 6 pH |
| Description | DNA ligase I, putative (EC 6.5.1.1). |
| Subcellular Location | nucleus[Predict] |
| E. C. Number | 6.5.1.1 |
| Sequence | >tr|Q4Q6U5|Q4Q6U5_LEIMA DNA ligase I, putative (EC 6.5.1.1) - Leishmania major ALVADVPPPTATSMAKLFQESSFDPVTTFEAVWLPPRVPAAPPTAGASEPVPFAAVVDVL ADISATGSRLECLKQLTFLLLAVIERCPEDLVPVMYLVINKHAPQHEGVELGIGDAVLVK AVAECCGMTETRAKEEYRQSGDLAEIAQMHKQKQGTLMKPKPLSARSVFKTYKEIAMMSG RDVMRRRSDLIKGLLRDAQGPEVNLIVRGLQQKMRIGLAEPSALAAVGYAFALHFLGGAQ MHKMDEVQLQTLLNTGADSLARIFYEVPSLDVVLSAVLANGFMTLVPGSSIAKQYAKDLS IRPGLPVKPQLAYPTSSITVILDRLQGKKFTSEYKYDGERAQIHYDKDKGFHIFSRNSET HTGKYPDVISMLPKVFDPVEVQSFILDSEVVAVHSETGALQAFQVLQHRGRKNIAEEDVS IPVCVFVFDILYFNGEPQLNKTLQQRRELLWRCIHPLPAKLSFATHLDSEKVEDVQTFLE RSIADGCEGLMVKTLEEEANYTPAKRSHYWLKLKKDYMDGVTDTLDLVPIAAFYGKGKRT GVFGGFLLACYDPKADEYQSICKIGTGFQDEELEKLTQSLKPFVVDEKPRYYRAGGEEPD VWLTEAQVWEVKAADLSVSPVHQAAVGLVDPDKGIALRFPRYLRQREDKKPADATNARQV ADMYKAQSLAAEHDANGDAE |
| DNA Sequence | >LmjF30.3440 |||DNA ligase I, putative|Leishmania major|chr 30|||Manual ATGGCGCTGG TCGCCGATGT GCCGCCGCCG ACGGCGACGT CCATGGCGAA GCTCTTCCAGGAGTCCTCCT TCGATCCTGT GACGACGTTC GAGGCAGTAT GGTTGCCTCC CCGGGTTCCTGCCGCGCCGC CTACCGCTGG TGCATCGGAG CCGGTGCCGT TTGCGGCAGT AGTGGACGTGCTGGCAGACA TCAGCGCCAC CGGCTCGCGG CTTGAGTGCT TGAAGCAGCT TACCTTTCTGCTGCTTGCTG TTATCGAGCG GTGCCCAGAG GACTTGGTGC CGGTGATGTA CCTGGTGATCAACAAGCATG CGCCGCAGCA CGAGGGCGTG GAGCTAGGCA TCGGTGATGC GGTGCTGGTGAAGGCGGTGG CCGAGTGCTG CGGCATGACG GAGACGCGAG CCAAAGAGGA GTACCGGCAGAGCGGCGATT TGGCGGAAAT CGCGCAGATG CATAAGCAGA AGCAAGGTAC GCTCATGAAACCGAAGCCGC TCTCCGCTCG GAGCGTGTTC AAGACATACA AGGAGATCGC TATGATGAGCGGCAGGGATG TGATGCGGCG GCGGTCGGAC TTGATCAAGG GGCTTTTGCG CGACGCACAGGGCCCGGAGG TGAACTTGAT TGTGCGTGGG CTTCAGCAGA AGATGCGCAT TGGCCTTGCCGAGCCATCCG CCTTGGCAGC TGTGGGGTAC GCCTTCGCTC TTCACTTCCT CGGTGGCGCGCAAATGCACA AGATGGACGA GGTGCAGCTG CAGACGCTGC TTAATACCGG CGCCGACAGTCTCGCGCGTA TCTTCTACGA GGTTCCAAGT CTCGACGTGG TTCTGAGCGC GGTGCTTGCGAACGGCTTCA TGACCTTGGT GCCGGGTAGC TCCATCGCGA AGCAGTATGC AAAGGACTTGTCCATTCGGC CTGGGCTGCC GGTGAAGCCC CAGTTAGCGT ACCCGACGAG CAGTATCACCGTCATCCTGG ACCGCCTGCA GGGCAAGAAG TTCACGTCGG AGTACAAGTA CGACGGTGAGCGCGCGCAGA TCCACTATGA CAAAGACAAA GGCTTCCATA TCTTTTCCCG CAACTCCGAGACTCACACCG GCAAATACCC GGACGTCATT TCGATGCTGC CAAAAGTCTT TGATCCAGTCGAAGTGCAGT CTTTCATCCT CGACTCGGAG GTTGTGGCGG TGCATTCGGA GACCGGTGCCCTTCAGGCCT TCCAAGTGCT GCAGCATCGT GGCCGAAAGA ACATTGCAGA GGAGGATGTCAGCATTCCCG TCTGCGTGTT TGTGTTTGAC ATCCTGTACT TCAACGGAGA GCCGCAGCTGAATAAGACAC TGCAGCAGCG TCGCGAGCTG CTGTGGCGGT GCATCCATCC CCTCCCGGCAAAGCTGTCGT TTGCCACGCA CTTGGATTCG GAGAAGGTCG AGGACGTGCA AACGTTTTTGGAGAGGTCCA TCGCGGACGG CTGCGAAGGA TTAATGGTGA AGACGCTCGA GGAGGAAGCCAACTACACCC CCGCCAAGCG CTCGCACTAC TGGCTGAAGC TGAAGAAGGA CTACATGGACGGCGTCACCG ATACGCTGGA TCTTGTCCCC ATCGCCGCTT TCTACGGCAA GGGCAAGCGAACTGGCGTGT TTGGCGGCTT CCTGCTGGCG TGCTACGACC CGAAGGCGGA TGAGTACCAGAGCATCTGCA AAATCGGCAC CGGCTTCCAG GACGAAGAGC TGGAGAAGCT CACGCAGTCCCTTAAGCCGT TCGTGGTGGA CGAAAAGCCG CGCTACTACC GCGCGGGTGG GGAGGAACCGGATGTTTGGC TCACCGAGGC GCAGGTGTGG GAGGTGAAGG CGGCGGACCT GTCAGTGTCGCCAGTTCATC AGGCAGCCGT GGGGCTCGTG GACCCCGACA AGGGCATCGC GCTGCGCTTTCCTCGCTACC TGCGCCAGCG CGAGGACAAG AAACCCGCAG ACGCCACAAA CGCACGGCAGGTGGCGGACA TGTACAAAGC GCAGTCCTTA GCAGCCGAAC ACGACGCGAA CGGGGACGCCGAGTGA |
| DNA ligase I, putative Q4Q6U5] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; DNA binding; DNA ligase (ATP) activity; nucleic acid binding |
| Biochemical Pathway | DNA recombination; DNA repair; DNA replication |
| Regulatory Pathway | |
| KEGG Pathways | K01971 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | DNA ligase I [Homo sapiens] | 44 | 1e-144 | 509 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| aspS aspartyl-tRNA synthetase | Staphylococcus aureus | 19% | 1 | 29.6 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00295 | ATP-dependent DNA ligase signatures and profile | 489-515; | PS00333 | |
| PDOC00295 | ATP-dependent DNA ligase signatures and profile | 334-342; | PS00697 | |
| PDOC00295 | ATP-dependent DNA ligase signatures and profile | 44.175 | 417-553 | PS50160 |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 441-444; | PS00722 | |
| Acylation | N-myristoylation site | 109-114; 113-118; 128-133; 289-294; 399-404; 521-526; 542-547; | PS00008 | |
| Amidation | Amidation site | 327-330; 410-413; 537-540; | PS00009 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 186-189; 329-332; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 22-25; 28-31; 69-72; 172-175; 180-183; 256-259; 466-469; 478-481; 483-486; 495-498; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 165-167; 172-174; 180-182; 301-303; 363-365; 470-472; 580-582; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 330-337; 494-502; | PS00007 | |
| DNA ligase I, putative [Q4Q6U5] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1x9nA | |
| Percent Identity | 44% | |
| Target Region | 20-669 | |
| Template Region | 262-632 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| LYS | 317 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 61-19-8 | ADENOSINE MONOPHOSPHATE | 347.221 | C10 H14 N5 O7 P | O=P(O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O | 1x9n |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/624834/Q4Q6U5.pdb 2.0 650 = residues | | = | *| Ramachandran plot: 90.2% core 9.1% allow 0.5% gener 0.2% = disall | | = | +| All Ramachandrans: 16 labelled residues (out of 648) = | +| Chi1-chi2 plots: 1 labelled residues (out of 380) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 4 | *| Bond len/angle: 7.8 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.03 Covalent: -0.17 Overall: = -0.04 | | = | | M/c bond lengths: 98.8% within limits 1.2% highlighted = | | M/c bond angles: 93.6% within limits 6.4% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |