| S-adenosylmethionine synthetase [Q4Q6T6] | |
|---|---|
| Systematic Name | LmjF.30.3500 [Leishmania major] |
| Gene Name | METK2 |
| Molecular Weight | 43017 Da |
| Protein Sequence Size | 392 |
| Function | |
| Charge | -4.5 |
| Isoelectric Point | 5.6662 pH |
| Description | S-adenosylmethionine synthetase (AdoMet synthetase) (Methionine adenosyltransferase). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 2.5.1.6 |
| Sequence | >tr|Q4Q6T6|Q4Q6T6_LEIMA S-adenosylmethionine synthetase (AdoMet synthetase) (Methionine adenosyltransferase) - Leishmania major. SVHSILFSSEHVTEGHPDKLCDQVSDAVLDACLAGDPFSKVACESCAKTGMVMVFGEITT KTVLDYQKIVRNTIKDIGFDSADKGLDYESCNVLVAIEQQSPDICQGLGNFDSEDLGAGD QGMMFGYATDETETLMPLTYELARGLAKKYSELRRVGSLEWARPDAKTQVTVEYDYDTRE GKQVLTPKRVAVVLISAQHDEHVTNDKISVDLMEKVIKAVIPANMLDAETKYCLNPSGRF VRGGPHGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDPSKVDRSAAYAARWIAKSIVAGGL ARRCLVQLAYAIGVAEPLSMHVETYGTGKYDDAKLLEIVKQNFKLRPYDIIQELNLRRPI YYDTSRFGHFGRKDESGTGGFTWEVPKKMVE |
| DNA Sequence | >LmjF30.3500 |METK1|MAT2|S-adenosylmethionine synthetase|Leishmania major|chr 30|||Manual ATGTCTGTCC ACAGCATTCT CTTCTCCTCC GAGCACGTGA CGGAGGGCCA TCCAGACAAGCTGTGTGACC AGGTATCCGA CGCCGTGCTT GACGCGTGCC TCGCCGGCGA CCCGTTCTCGAAGGTTGCGT GCGAGTCGTG CGCGAAAACG GGCATGGTGA TGGTGTTCGG CGAGATCACGACGAAGACAG TGCTAGACTA CCAGAAGATC GTCCGCAACA CGATCAAGGA CATTGGCTTCGATTCCGCGG ACAAGGGTCT GGACTACGAG TCGTGCAATG TGCTGGTTGC GATTGAGCAGCAGTCGCCGG ACATCTGCCA GGGTCTGGGC AACTTCGATA GCGAGGATCT CGGCGCTGGCGACCAGGGCA TGATGTTCGG CTACGCGACG GACGAGACGG AGACGCTGAT GCCGCTGACGTACGAGCTGG CCCGCGGCCT TGCGAAGAAG TACAGCGAGC TTCGCCGCGT CGGCAGCCTGGAGTGGGCTC GCCCGGACGC GAAGACGCAG GTGACGGTGG AGTACGACTA CGACACGCGCGAGGGCAAGC AGGTGCTGAC GCCGAAGCGC GTGGCGGTGG TGCTGATCTC TGCGCAGCACGACGAGCACG TGACCAACGA TAAGATCAGC GTAGATCTGA TGGAGAAGGT GATCAAGGCTGTGATCCCTG CGAACATGCT GGACGCGGAG ACGAAGTACT GTCTGAATCC ATCTGGCCGCTTCGTGCGCG GTGGGCCGCA TGGCGACGCC GGCCTGACTG GCCGAAAGAT CATCGTAGACACGTACGGCG GCTGGGGCGC GCACGGCGGT GGCGCCTTCT CCGGCAAGGA CCCTTCGAAGGTGGACCGCT CCGCCGCGTA CGCCGCGCGC TGGATCGCGA AGTCGATCGT TGCAGGTGGCCTGGCGCGCC GCTGCCTTGT GCAGCTCGCG TACGCCATCG GTGTAGCGGA GCCGCTGAGCATGCACGTGG AGACGTACGG CACCGGCAAG TACGATGACG CGAAGCTGCT GGAGATCGTGAAGCAGAACT TCAAGCTGCG CCCGTACGAC ATCATCCAGG AGCTGAACCT GCGCCGCCCCATCTACTACG ATACGTCGCG CTTCGGCCAC TTCGGCCGTA AGGACGAGTC GGGCACGGGCGGGTTCACTT GGGAGGTGCC AAAGAAGATG GTCGAGTAA |
| S-adenosylmethionine synthetase Q4Q6T6] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; methionine adenosyltransferase activity |
| Biochemical Pathway | one-carbon compound metabolism |
| Regulatory Pathway | |
| KEGG Pathways | K00789 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | methionine adenosyltransferase II, alpha [Homo sapiens] | 58 | 1e-125 | 444 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| metK S-adenosylmethionine synthetase | Staphylococcus aureus | 52% | 1e-111 | 397 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00369 | S-adenosylmethionine synthetase signatures | 118-128; | PS00376 | |
| PDOC00369 | S-adenosylmethionine synthetase signatures | 269-277; | PS00377 | |
| Acylation | N-myristoylation site | 110-115; 123-128; 244-249; 263-268; 266-271; 270-275; 271-276; | PS00008 | |
| Amidation | Amidation site | 253-256; 371-374; | PS00009 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 149-152; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 63-66; 74-77; 274-277; 279-282; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 60-62; 74-76; 187-189; 238-240; 253-255; 274-276; 328-330; 365-367; | PS00005 | |
| Sulfation | Tyrosine sulfation site | 82-96; 168-182; 170-184; | PS00003 | |
| S-adenosylmethionine synthetase [Q4Q6T6] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2p02A | |
| Percent Identity | 58% | |
| Target Region | 1-392 | |
| Template Region | 38-380 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| HIS | 17 | Unknown |
| LYS | 168 | Unknown |
| ARG | 255 | Unknown |
| LYS | 256 | Unknown |
| LYS | 276 | Unknown |
| LYS | 280 | Unknown |
| ASP | 282 | Unknown |
| Co-Factor | |
|---|---|
| Metal | Description |
| K | Binds 1 potassium ion per subunit |
| Magnesium or cobalt | Binds 2 divalent ions per subunit |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 16887-00-6 | CHLORIDE ION | 35.453 | Cl | [Cl-] | 2p02 |
| 86866-89-9 | S-ADENOSYLMETHIONINE | 398.437 | C15 H22 N6 O5 S | [O-]C(=O)C(N)CC[S+](C)CC3OC(n2cnc1c(ncnc12)N)C(O)C3O | 2p02 |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/3804800/Q4Q6T6.pdb 2.0 392 = residues | | = | +| Ramachandran plot: 93.5% core 6.2% allow 0.3% gener 0.0% = disall | | = | +| All Ramachandrans: 6 labelled residues (out of 390) = | +| Chi1-chi2 plots: 2 labelled residues (out of 222) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 15.6 Bad contacts: = 2 | *| Bond len/angle: 7.6 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.07 Covalent: -0.16 Overall: = -0.01 | | = | | M/c bond lengths: 99.6% within limits 0.4% highlighted = | | M/c bond angles: 94.4% within limits 5.6% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |