| DNA-directed RNA polymerase II subunit 2, putative [Q4Q6P8] | |
|---|---|
| Systematic Name | LmjF.31.0160 [Leishmania major] |
| Gene Name | LMJF_31_0160 |
| Molecular Weight | 133840 Da |
| Protein Sequence Size | 1185 |
| Function | |
| Charge | 19.5 |
| Isoelectric Point | 7.6 pH |
| Description | DNA-directed RNA polymerase II subunit 2, putative (EC 2.7.7.6). |
| Subcellular Location | nucleus[Predict] |
| E. C. Number | 2.7.7.6 |
| Sequence | >tr|Q4Q6P8|Q4Q6P8_LEIMA DNA-directed RNA polymerase II subunit 2, putative (EC 2.7.7.6) - Leishmania major AQDELLDLEDPDIVLDDGIVDEHDDENPNDDLDSEDIWEVISSFFREKGLVHQQLDSYND FLMRIPKMIESMAVIPRQDDQYDPGVTMEEQRDQPRLVLQDVVIGNPSHQTHSYFTGGPL PPLFPNECRLRDMTYDAQAQVTLGVELYHPHADTPHDTFLRNVELGRIPIMLKSMRCNLS GKDEDELPRLNECPHDQGGYFVINGTEKVLIAQERQAANHVYTFSRQKGLLCEVKSIVEG SLNKPRTLQIIMQYRNKGPGSGFENLMCRVAQMDETIPLFVLFRAMDMVADKEILQTVVP DLKDTAMLELLRGSMSDASTLQIFTREEALGYIGRRLGKQDAMANLQREAESLLMRDLLP HMGTDPSANRTKCLYMGYMVHKLLLVALGRHEETDRDFLGHKRIDVAGTLLTVQLQTFLA QVRKEMIKTLQDHAANPRGVFSFGRVIHSKLITDGLRRCLATGNFGDLKTGNIKTGVAQT LNRLTYSSSLSNLRRIQNPIAASSKATRPRNLHCTQWGYICPVETPEGGSIGLLKNVALM CLMSRGTDHAEVVQAVQARIDGFRTIGLEDTADVRVARVFVNGTMIGVDRNPERLLHDLR TRRRNGELSNEVSIVRDIRDREIRVFSDAGRCLRPLFVAENCRLKLRKSGMRDLLVSEKA GGKKSISWKTALKKGYVELIDCEEEDSLLIAMTPNEVEKNYYYSHCEMDPSMILGICASI IPYPNHNQSPRNTYQSAMGKQAMGIYASNFNMRMDTTAHVLFYPQKPLVRTKAMTYMHSN DLPAGHNAVVAISCYSGYNQEDSIIMSRSAVERGFFRSAFWRSFKAKEERQKRDALETFE NPDRELCRVKRADYSKLDTDGLIKPGMRVLGGDVVVGKTIPLPEADLEKLTTGNTKITKR DASITSRTTEKGVVDKVMLTMNNNDRFTKVKIRTIKIPNIGDKFCSRHGQKGTNGIQFRQ EDMPFNRDGIVPDLIINPHAIPSRMTVAHLIETLAGKVACYKGGEVYATPFCSVVVDDFG KALHALRSQRYGNECLYNGHTGLPLDHLIFFGPTFYQRLKHLSGDKIHARPRGPLQPLVR QPTEGRAHEGGLRFGEMERDCMLSYGASQWLRERLFRVSDYYTVHVCNMCGTICVADTKL NRYQCKGCENDTRISQVLMPYACKLLFQELMSMTILPRLGTGPL |
| DNA Sequence | >LmjF31.0160 |||DNA-directed RNA polymerase II subunit 2, putative|Leishmania major|chr 31|||Manual ATGGCACAGG ATGAGCTGCT GGATCTGGAA GACCCTGACA TAGTCCTCGA TGACGGCATCGTCGACGAGC ACGACGACGA GAACCCCAAC GATGACCTCG ACAGCGAGGA CATTTGGGAGGTCATCTCAT CCTTTTTCCG GGAGAAGGGT CTGGTGCACC AGCAGCTCGA CTCGTATAACGACTTCCTCA TGCGCATCCC CAAGATGATT GAGAGCATGG CGGTCATCCC GCGCCAAGACGACCAGTACG ACCCGGGTGT CACAATGGAG GAGCAGCGCG ACCAGCCCCG GCTGGTGTTGCAGGACGTGG TGATTGGCAA CCCGTCGCAC CAGACGCACT CGTACTTCAC AGGTGGCCCGCTCCCGCCGC TCTTCCCGAA CGAGTGCCGT CTGCGCGACA TGACGTACGA CGCACAGGCGCAGGTGACAC TCGGAGTAGA GCTGTACCAC CCCCACGCGG ACACGCCGCA CGACACCTTCCTGCGCAACG TCGAGCTGGG ACGCATTCCC ATCATGCTCA AGTCTATGCG CTGCAATCTCAGTGGCAAGG ATGAGGATGA GCTGCCGCGG CTCAACGAGT GCCCGCATGA CCAGGGCGGGTACTTCGTGA TCAACGGTAC CGAGAAGGTG CTCATTGCGC AGGAGCGGCA GGCCGCCAACCACGTCTACA CGTTTTCCCG GCAGAAAGGG CTGTTGTGCG AGGTGAAGTC GATCGTGGAGGGCTCGCTGA ACAAGCCGCG CACGCTGCAG ATCATCATGC AGTACAGGAA CAAAGGTCCGGGCAGCGGCT TCGAGAACTT GATGTGCCGC GTTGCGCAGA TGGACGAGAC AATTCCGCTTTTTGTGCTGT TCCGGGCGAT GGACATGGTG GCAGACAAGG AGATTCTGCA GACGGTCGTGCCGGATCTGA AGGACACGGC GATGCTGGAG CTGCTGCGTG GCTCCATGAG CGACGCGAGCACGCTGCAGA TCTTCACCCG CGAGGAGGCC CTTGGCTACA TTGGACGCCG TCTCGGCAAGCAAGACGCCA TGGCAAACCT GCAACGGGAG GCGGAGAGCC TGCTCATGCG GGACTTACTGCCGCACATGG GCACGGACCC GTCCGCCAAC CGCACCAAGT GCCTGTACAT GGGCTACATGGTGCACAAGC TGCTGCTCGT CGCCCTCGGC CGGCACGAGG AGACGGACCG CGATTTTCTCGGGCACAAGC GCATTGACGT GGCAGGCACG CTGCTGACGG TGCAGCTGCA GACCTTCCTTGCGCAGGTGC GGAAGGAGAT GATCAAGACA CTGCAGGACC ACGCCGCCAA CCCGCGCGGCGTCTTCAGCT TCGGTCGCGT CATTCACAGC AAACTTATCA CAGACGGTCT GCGCCGCTGCCTCGCCACCG GCAACTTCGG CGACCTCAAG ACGGGCAACA TCAAGACCGG CGTGGCGCAGACGCTGAACC GCCTCACCTA CTCGTCCTCC CTTAGCAATC TGCGCCGCAT CCAGAACCCCATTGCCGCCT CCAGCAAGGC GACGCGGCCG CGCAACTTGC ACTGCACGCA GTGGGGGTACATCTGCCCCG TGGAGACGCC AGAGGGTGGC TCGATCGGGC TGCTCAAGAA TGTGGCGCTGATGTGTCTCA TGTCCCGCGG CACGGACCAC GCCGAGGTGG TGCAGGCCGT GCAGGCGCGCATCGATGGCT TCCGCACGAT CGGCCTCGAG GACACGGCTG ACGTGCGCGT GGCGCGCGTGTTTGTGAACG GAACGATGAT TGGCGTCGAC CGCAACCCGG AGCGGCTGTT GCACGACCTTCGCACGCGGC GCCGCAACGG CGAGCTCAGC AACGAGGTGA GCATCGTGCG CGATATCCGCGATCGCGAAA TTCGCGTCTT CTCGGACGCT GGTCGCTGCC TGCGCCCTCT CTTCGTTGCCGAAAACTGCC GCCTGAAGCT CCGCAAGTCC GGCATGCGCG ACCTGCTTGT CTCCGAGAAGGCCGGCGGGA AGAAGTCGAT CTCGTGGAAG ACCGCGTTGA AGAAGGGCTA CGTCGAGCTGATTGACTGCG AGGAGGAGGA CTCACTGCTG ATCGCCATGA CGCCAAACGA GGTGGAGAAGAACTACTACT ATTCCCACTG CGAGATGGAC CCGTCGATGA TTCTCGGCAT TTGCGCCTCCATTATCCCGT ACCCCAACCA CAACCAGTCC CCGCGCAACA CCTACCAGTC CGCCATGGGTAAGCAGGCCA TGGGCATCTA CGCCTCGAAC TTCAACATGC GCATGGACAC CACCGCCCACGTCCTCTTCT ACCCGCAAAA ACCGCTGGTG CGTACCAAGG CTATGACGTA CATGCACAGCAACGATCTGC CCGCCGGCCA CAACGCGGTG GTGGCCATCT CGTGCTACAG TGGGTACAACCAAGAGGACT CCATCATCAT GAGCCGCTCC GCCGTGGAGC GCGGCTTCTT CCGCAGCGCGTTCTGGCGCT CCTTCAAGGC GAAGGAGGAG CGGCAGAAGC GCGACGCGCT CGAGACATTTGAGAACCCCG ACCGCGAGCT GTGCCGTGTG AAGCGCGCCG ACTATTCGAA GCTGGACACGGACGGCCTCA TCAAGCCCGG CATGCGGGTC CTCGGTGGCG ACGTGGTGGT GGGCAAGACGATCCCTCTCC CTGAGGCGGA CCTCGAGAAG CTCACGACCG GCAACACCAA GATCACGAAGCGTGACGCGA GCATCACCTC TCGCACCACG GAGAAGGGTG TCGTGGACAA GGTAATGCTGACCATGAACA ACAACGACCG CTTCACAAAG GTCAAGATTC GCACCATCAA GATCCCTAACATCGGCGACA AATTCTGCAG CCGCCACGGA CAAAAGGGCA CCAACGGCAT CCAGTTTCGCCAGGAGGACA TGCCCTTCAA CCGCGACGGC ATCGTGCCGG ACCTCATCAT CAACCCCCACGCCATTCCCT CCCGCATGAC AGTGGCCCAC CTGATCGAAA CGCTCGCCGG CAAGGTGGCGTGCTACAAGG GCGGCGAGGT CTACGCAACG CCGTTCTGCT CCGTCGTTGT GGACGACTTTGGCAAGGCTT TACATGCCCT CCGGTCGCAG CGGTACGGCA ACGAGTGCCT CTACAATGGGCACACCGGTC TGCCGCTGGA CCATCTGATC TTCTTCGGCC CCACCTTCTA CCAGCGCCTGAAGCACTTAT CCGGTGACAA GATCCACGCA CGCCCACGCG GGCCGCTGCA GCCGCTGGTGCGCCAGCCAA CCGAGGGTCG CGCACACGAG GGTGGGCTGC GGTTTGGCGA GATGGAGCGCGATTGCATGC TGTCCTACGG CGCCTCGCAG TGGCTGCGGG AGCGCCTTTT CCGCGTCAGCGACTACTACA CGGTACACGT CTGCAACATG TGCGGCACCA TATGCGTGGC CGACACAAAGCTAAACCGCT ACCAATGCAA GGGCTGCGAA AACGACACGC GCATCTCCCA GGTACTCATGCCGTACGCCT GCAAGCTGCT CTTCCAGGAG CTCATGTCCA TGACGATCCT GCCGCGCCTCGGTACCGGTC CTCTGTAA |
| DNA-directed RNA polymerase II subunit 2, putative Q4Q6P8] | |
|---|---|
| Metabolite Information | |
| Molecular Function | DNA binding; DNA-directed RNA polymerase activity |
| Biochemical Pathway | transcription |
| Regulatory Pathway | |
| KEGG Pathways | K03010 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | DNA directed RNA polymerase II polypeptide B [Homo sapiens] | 45 | 0 | 1005 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YOR151c DNA-directed RNA polymerase II, 140 kDa chain | Saccharomyces cerevisiae | 44% | 0 | 957 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00017 | ATP/GTP-binding site motif A (P-loop) | 873-880; | PS00017 | |
| PDOC00018 | EF-hand calcium-binding domain signature and profile | 26-38; | PS00018 | |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 179-182; 205-208; 370-373; 583-586; 1151-1154; | PS00722 | |
| PDOC00896 | RNA polymerases beta chain signature | 942-954; | PS01166 | |
| Acylation | N-myristoylation site | 364-369; 409-414; 456-461; 472-477; 477-482; 529-534; 533-538; 547-552; 568-573; 584-589; 662-667; 716-721; 745-750; 950-955; 1092-1097; | PS00008 | |
| Amidation | Amidation site | 334-337; 662-665; | PS00009 | |
| Phosphorylation | Casein kinase II phosphorylation site | 58-61; 88-91; 155-158; 181-184; 237-240; 262-265; 315-318; 326-329; 395-398; 430-433; 694-697; 705-708; 810-813; 856-859; 899-902; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 175-177; 181-183; 207-209; 395-397; 504-506; 602-604; 658-660; 668-670; 730-732; 824-826; 899-901; 906-908; 910-912; 935-937; 1029-1031; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 130-136; 1031-1038; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 76-90; | PS00003 | |
| DNA-directed RNA polymerase II subunit 2, putative [Q4Q6P8] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2yu9B | |
| Percent Identity | 44% | |
| Target Region | 28- 1208 | |
| Template Region | 20-1121 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 2yu9 |
| 58-98-0 | URIDINE 5'-TRIPHOSPHATE | 484.141 | C9 H15 N2 O15 P3 | O=P(O)(O)OP(=O)(O)OP(=O)(O)OCC2OC(N1C(=O)NC(=O)C=C1)C(O)C2O | 2yu9 |
| 23713-49-7 | ZINC ION | 65.409 | Zn | [Zn+2] | 2yu9 |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/535250/Q4Q6P8.pdb 2.0 1158 = residues | | = | *| Ramachandran plot: 85.2% core 11.4% allow 2.4% gener 1.1% = disall | | = | *| All Ramachandrans: 73 labelled residues (out of1156) = | +| Chi1-chi2 plots: 10 labelled residues (out of 721) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 5.6 Bad contacts: = 37 | *| Bond len/angle: 8.1 Morris et al class: 1 = 1 2 | *| 4 cis-peptides = | | G-factors Dihedrals: -0.07 Covalent: -0.21 Overall: = -0.12 | | = | | M/c bond lengths: 98.9% within limits 1.1% highlighted = | *| M/c bond angles: 93.1% within limits 6.9% highlighted 2 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |