| Prostaglandin f2-alpha synthase [Q4Q646] | |
|---|---|
| Systematic Name | LmjF.31.2150 [Leishmania major] |
| Gene Name | |
| Molecular Weight | 31850 Da |
| Protein Sequence Size | 284 |
| Function | |
| Charge | -1.5 |
| Isoelectric Point | 6.1736 pH |
| Description | Prostaglandin f2-alpha synthase. |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4Q646|Q4Q646_LEIMA Prostaglandin f2-alpha synthase - Leishmania major. AGVDKAMVTLSNGVKMPQFGLGVWQSPAGEVTENAVKWALCAGYRHIDTAAIYKNEESVG AGLRASGVPREDVFITTKLWNTEQGYESTLAAFEESRQKLGVDYIDLYLIHWPRGKDILS KEGKKYLDSWRAFEQLYKEKKVRAIGVSNFHIHHLEDVLAMCTVTPMVNQVELHPLNNQA DLRAFCDAKQIKVEAWSPLGQGKLLSNPILSAIGAKYNKTAAQVILRWNIQKNLITIPKS VHRERIEENADIFDFELGAEDVMSIDALNTNSRYGPDPDEAQF |
| DNA Sequence | >LmjF31.2150 |PGFS||prostaglandin f2-alpha synthase|Leishmania major|chr 31|||Manual ATGGCTGGCG TTGATAAGGC AATGGTCACG CTGAGCAACG GCGTCAAGAT GCCGCAGTTCGGTCTCGGCG TGTGGCAGTC CCCCGCCGGC GAGGTAACCG AGAACGCTGT CAAGTGGGCGCTGTGTGCCG GCTACCGCCA CATCGACACG GCAGCCATCT ACAAGAACGA GGAGAGCGTCGGGGCTGGGC TGCGTGCCTC TGGTGTACCG CGCGAGGACG TGTTCATCAC GACGAAGCTCTGGAACACGG AGCAGGGCTA CGAGAGCACG CTTGCGGCCT TCGAGGAGAG TCGACAGAAGCTCGGTGTCG ACTACATTGA TCTCTACCTC ATCCACTGGC CGCGCGGCAA AGATATCCTGTCGAAAGAGG GCAAGAAGTA CCTGGATTCG TGGCGCGCCT TCGAGCAGCT CTACAAGGAGAAGAAGGTGC GGGCGATTGG AGTGTCGAAC TTTCACATCC ACCATCTGGA GGACGTGCTTGCAATGTGCA CTGTGACGCC AATGGTGAAC CAGGTGGAGC TTCACCCGTT GAACAATCAGGCCGATCTGC GGGCCTTCTG CGACGCCAAG CAAATCAAAG TTGAGGCTTG GTCGCCGCTAGGGCAGGGCA AGCTGCTCTC CAACCCGATC CTCTCCGCAA TCGGTGCAAA GTACAATAAGACGGCCGCGC AGGTGATCCT CCGCTGGAAC ATCCAGAAGA ATCTCATCAC GATCCCCAAGTCAGTCCACA GGGAGCGTAT CGAGGAGAAC GCGGACATCT TCGACTTTGA GCTCGGCGCCGAGGACGTGA TGAGTATCGA CGCTCTCAAC ACGAACTCGC GCTACGGCCC CGACCCTGATGAGGCGCAGT TCTAA |
| Prostaglandin f2-alpha synthase Q4Q646] | |
|---|---|
| Metabolite Information | biological process unknown |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | aldo-keto reductase family 1, member A1 [Homo sapiens] | 40 | 5e-58 | 221 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| Rv2971 PROBABLE OXIDOREDUCTASE | Mycobacterium tuberculosis H37Rv | 43% | 3e-57 | 215 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00061 | Aldo/keto reductase family signatures | 134-151; | PS00062 | |
| PDOC00061 | Aldo/keto reductase family signatures | 238-253; | PS00063 | |
| PDOC00061 | Aldo/keto reductase family signatures | 44-61; | PS00798 | |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 219-222; | PS00722 | |
| Acylation | N-myristoylation site | 3-8; 63-68; 215-220; | PS00008 | |
| Amidation | Amidation site | 123-126; | PS00009 | |
| Phosphorylation | Protein kinase C phosphorylation site | 77-79; 130-132; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 132-138; | PS00007 | |
| Prostaglandin f2-alpha synthase [Q4Q646] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1vbjB | |
| Percent Identity | 61% | |
| Target Region | 1-284 | |
| Template Region | 1-279 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 49 | Sidechain |
| TYR | 54 | Sidechain |
| LYS | 79 | Sidechain |
| HIS | 112 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 5949-29-1 | CITRIC ACID | 192.124 | C6 H8 O7 | O=C(O)CC(O)(C(=O)O)CC(=O)O | 1vbj |
| NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | 743.405 | C21 H28 N7 O17 P3 | c1cc(c[n+](c1)C2C(C(C(O2)COP(=O)([O-])OP(=O)(O)OCC3C(C(C(O3)n4cnc5c4ncnc5N)OP(=O)(O)O)O)O)O)C(=O)N | 1vbj | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/5147731/Q4Q646.pdb 2.0 284 = residues | | = | +| Ramachandran plot: 91.3% core 8.3% allow 0.4% gener 0.0% = disall | | = | +| All Ramachandrans: 3 labelled residues (out of 282) = | +| Chi1-chi2 plots: 3 labelled residues (out of 177) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.9 Bad contacts: = 3 | *| Bond len/angle: 5.7 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.09 Covalent: -0.08 Overall: = 0.03 | | = | | M/c bond lengths: 99.6% within limits 0.4% highlighted = | | M/c bond angles: 95.1% within limits 4.9% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |