| Prostaglandin f synthase, putative [Q4Q5N9] | |
|---|---|
| Systematic Name | LmjF.32.0460 [Leishmania major] |
| Gene Name | LMJF_32_0460 |
| Molecular Weight | 31834 Da |
| Protein Sequence Size | 279 |
| Function | |
| Charge | 4.5 |
| Isoelectric Point | 7.3605 pH |
| Description | Prostaglandin f synthase, putative. |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4Q5N9|Q4Q5N9_LEIMA Prostaglandin f synthase, putative - Leishmania major AVKCTHVTLSNGVQVPQLGIGTWEAKDGNEVVNIKWAVNAGYRHVDTAHYYKNEKGVGQG ISECGVPRSDIFVTTKLWNYDHGYESALAAFEQSRQALGVEYVDLYLIHWPGPNRSYIET WRAFEKLYEMKKVRAIGVSNFEPHHLDDLLANCTVPPMVNQVEMHPHFQQKALRAYCAEK NIAVTAWRPLGKGALLTEPQLVELAEKHKRSAAQVIIRWLIQLGVIAIPKSSHEERIKQN FDVFDFELSPEDMRRIESMDRNSRIGSSPETFFPTERK |
| DNA Sequence | >LmjF32.0460 |||prostaglandin f synthase, putative|Leishmania major|chr 32|||Manual ATGGCTGTTA AGTGCACGCA CGTGACCCTG AGCAATGGGG TACAGGTGCC ACAGCTCGGCATCGGCACGT GGGAGGCGAA GGATGGCAAT GAAGTCGTGA ACATCAAGTG GGCTGTCAACGCTGGCTACC GCCATGTTGA CACTGCGCAC TACTACAAGA ACGAGAAGGG CGTTGGCCAGGGCATTAGCG AATGCGGCGT GCCGCGCTCT GATATCTTCG TCACGACGAA GCTGTGGAACTACGACCACG GCTACGAAAG TGCCCTCGCT GCCTTCGAGC AGAGTCGCCA AGCGCTTGGGGTGGAGTACG TGGATTTGTA CCTCATCCAC TGGCCGGGCC CCAATCGCTC GTATATCGAGACGTGGCGTG CCTTCGAGAA GTTGTATGAG ATGAAGAAGG TGCGTGCGAT CGGCGTGTCCAACTTTGAGC CGCACCACCT TGATGACCTG CTGGCGAACT GCACGGTGCC ACCGATGGTGAACCAGGTAG AGATGCACCC TCACTTCCAG CAAAAGGCGC TCCGCGCGTA CTGCGCCGAGAAGAACATTG CCGTGACGGC ATGGCGCCCT CTTGGCAAGG GTGCCCTGCT GACAGAGCCTCAGCTCGTGG AGCTGGCGGA AAAGCACAAG AGGTCTGCAG CTCAGGTCAT CATTCGCTGGCTCATCCAAC TCGGCGTCAT TGCCATCCCC AAGTCCAGCC ACGAGGAGCG CATCAAACAGAACTTCGACG TTTTCGACTT TGAGCTTTCA CCCGAGGACA TGCGGAGAAT CGAGTCCATGGACAGGAATT CGCGTATCGG GTCTAGCCCC GAGACCTTCT TCCCAACCGA GCGCAAGTAA |
| Prostaglandin f synthase, putative Q4Q5N9] | |
|---|---|
| Metabolite Information | biological process unknown |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K00002 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | aldo-keto reductase family 1, member A1 [Homo sapiens] | 39 | 2e-56 | 216 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| Rv2971 PROBABLE OXIDOREDUCTASE | Mycobacterium tuberculosis H37Rv | 37% | 2e-50 | 192 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00061 | Aldo/keto reductase family signatures | 125-142; | PS00062 | |
| PDOC00061 | Aldo/keto reductase family signatures | 229-244; | PS00063 | |
| PDOC00061 | Aldo/keto reductase family signatures | 42-59; | PS00798 | |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 115-118; 153-156; | PS00722 | |
| Acylation | N-myristoylation site | 22-27; 57-62; 59-64; 61-66; 66-71; 194-199; | PS00008 | |
| Phosphorylation | Casein kinase II phosphorylation site | 117-120; 140-143; 232-235; 233-236; 250-253; 268-271; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 75-77; 121-123; 276-278; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 44-51; 123-129; | PS00007 | |
| Prostaglandin f synthase, putative [Q4Q5N9] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1vbjB | |
| Percent Identity | 58% | |
| Target Region | 1-279 | |
| Template Region | 1-279 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 47 | Sidechain |
| TYR | 52 | Sidechain |
| LYS | 77 | Sidechain |
| HIS | 110 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 5949-29-1 | CITRIC ACID | 192.124 | C6 H8 O7 | O=C(O)CC(O)(C(=O)O)CC(=O)O | 1vbj |
| NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | 743.405 | C21 H28 N7 O17 P3 | c1cc(c[n+](c1)C2C(C(C(O2)COP(=O)([O-])OP(=O)(O)OCC3C(C(C(O3)n4cnc5c4ncnc5N)OP(=O)(O)O)O)O)O)C(=O)N | 1vbj | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1674156/Q4Q5N9.pdb 2.0 279 = residues | | = | | Ramachandran plot: 91.5% core 8.5% allow 0.0% gener 0.0% = disall | | = | +| All Ramachandrans: 4 labelled residues (out of 277) = | | Chi1-chi2 plots: 0 labelled residues (out of 172) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 5.2 Bad contacts: = 3 | *| Bond len/angle: 6.4 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.10 Covalent: -0.14 Overall: = 0.01 | | = | | M/c bond lengths: 99.2% within limits 0.8% highlighted = | | M/c bond angles: 94.3% within limits 5.7% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |