| Ras-related rab-4, putative [Q4Q5N4] | |
|---|---|
| Systematic Name | LmjF.32.0490 [Leishmania major] |
| Gene Name | LMJF_32_0490 |
| Molecular Weight | 22166 Da |
| Protein Sequence Size | 203 |
| Function | |
| Charge | -3.5 |
| Isoelectric Point | 5.0204 pH |
| Description | Ras-related rab-4, putative. |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4Q5N4|Q4Q5N4_LEIMA Ras-related rab-4, putative - Leishmania major ADKYQQLMKLIVIGDSGVGKSCLLHRFIEDTFSEEQTQTIGIEYGAKIIDVGGAKVKLQI WDTAGQERYKSVTRSYYRGATGCLIVYDVNNRSSYESVPQWLSDVRQLAGSDVVVMLIGN KMDLTKGNSVRAVQHNEASLYAQQNGLLHFETSAATGEFVSEAFLKVAKSAVSLASAAEP ESDTQLIQDNTANNSYLSSCSC |
| DNA Sequence | >LmjF32.0490 |||ras-related rab-4, putative|Leishmania major|chr 32|||Manual ATGGCGGACA AGTATCAGCA GTTGATGAAA CTCATTGTCA TCGGAGACAG TGGCGTGGGCAAGTCGTGCC TGCTGCACCG ATTCATTGAG GACACTTTTT CTGAAGAGCA GACGCAGACCATCGGCATTG AGTACGGTGC GAAGATTATC GACGTGGGCG GAGCAAAGGT CAAGCTGCAGATCTGGGACA CAGCTGGCCA GGAGCGGTAC AAGTCGGTGA CGAGAAGCTA TTATCGTGGTGCCACGGGAT GCCTCATCGT CTACGATGTC AACAATAGAA GCTCGTACGA AAGTGTTCCGCAGTGGTTAA GCGACGTACG ACAGCTAGCA GGAAGCGATG TCGTAGTAAT GCTCATCGGAAATAAAATGG ATCTGACGAA AGGGAATAGC GTTCGCGCAG TCCAGCACAA CGAGGCATCTTTGTATGCGC AGCAAAATGG TCTGCTGCAC TTTGAAACTT CCGCAGCTAC GGGCGAGTTTGTTTCTGAAG CTTTCCTGAA AGTCGCCAAA AGTGCCGTGT CCTTGGCGAG CGCTGCGGAGCCAGAGAGCG ACACCCAGCT GATCCAGGAT AACACAGCAA ACAACTCATA CCTTTCGTCGTGCTCTTGCT AA |
| Ras-related rab-4, putative Q4Q5N4] | |
|---|---|
| Metabolite Information | intracellular protein transport; protein transport; small GTPase mediated signal transduction |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K07976 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | RAB4A, member RAS oncogene family [Homo sapiens] | 56 | 2e-49 | 192 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YFL038c GTP-binding protein of the rab family | Saccharomyces cerevisiae | 45% | 1e-38 | 153 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00017 | ATP/GTP-binding site motif A (P-loop) | 15-22; | PS00017 | |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 92-95; 194-197; | PS00722 | |
| PDOC00579 | Sigma-54 interaction domain signatures and profile | 11-24; | PS00675 | |
| Acylation | N-myristoylation site | 18-23; 42-47; 80-85; | PS00008 | |
| Phosphorylation | Casein kinase II phosphorylation site | 32-35; 94-97; 177-180; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 130-132; | PS00005 | |
| Ras-related rab-4, putative [Q4Q5N4] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2bmeD | |
| Percent Identity | 56% | |
| Target Region | 1-178 | |
| Template Region | 4-174 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| GLN | 67 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 60-24-2 | BETA-MERCAPTOETHANOL | 78.133 | C2 H6 O S | OCCS | 2bme |
| PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER | 522.196 | C10 H17 N6 O13 P3 | O=P(O)(O)NP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c2N=C(N)NC1=O)C(O)C3O | 2bme | |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 2bme |
| 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | 122.143 | C4 H12 N O3 | OCC([NH3+])(CO)CO | 2bme | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/117618/Q4Q5N4.pdb 2.0 178 = residues | | = | | Ramachandran plot: 96.9% core 3.1% allow 0.0% gener 0.0% = disall | | = | +| All Ramachandrans: 2 labelled residues (out of 176) = | | Chi1-chi2 plots: 0 labelled residues (out of 101) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | | Residue properties: Max.deviation: 2.9 Bad contacts: = 1 | +| Bond len/angle: 4.0 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.09 Covalent: -0.12 Overall: = 0.01 | | = | | M/c bond lengths: 99.9% within limits 0.1% highlighted = | | M/c bond angles: 93.9% within limits 6.1% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |