| Serine/threonine protein phosphatase [Q4Q4W8] | |
|---|---|
| Systematic Name | LmjF.32.3040 [Leishmania major] |
| Gene Name | LMJF_32_3040 |
| Molecular Weight | 34995 Da |
| Protein Sequence Size | 308 |
| Function | |
| Charge | -9.5 |
| Isoelectric Point | 4.8932 pH |
| Description | Serine/threonine protein phosphatase (EC 3.1.3.16). |
| Subcellular Location | protein phosphatase type 2A complex[Predict] |
| E. C. Number | 3.1.3.16 |
| Sequence | >tr|Q4Q4W8|Q4Q4W8_LEIMA Serine/threonine protein phosphatase (EC 3.1.3.16) - Leishmania major. LTEILDIDEHLATLNRCENIGEADVKRLCLKAKEIFTSEENVHKIPAPCTIVGDIHGQFY DLLELFRVGGEIPDTNYVFMGDFVDRGYHSVESFLLLLVLKIKYSRRVALVRGNHESRQI TQVYGFYDECLRKYGSINVWRYCTDVFDLLPLASVVEGKIFCVHAGLSPSIQTVDQMRSI RRNCEVPHEGAMCDLLWSDPEDIDGWGLSPRGAGYLFGGDVVCQFNETNKLDLICRSHQL VMEGYKAMFNDTLVTVWSAPNYCYRCGNVASILELDEHLNKNFKIFEAAPAEAREAGQRN EVPAYFL |
| DNA Sequence | >LmjF32.3040 |||protein phosphatase 2A catalytic subunit, putative|Leishmania major|chr 32|||Manual ATGTTAACAG AAATATTGGA CATTGACGAG CACCTCGCCA CCCTCAACCG GTGCGAGAACATCGGCGAGG CGGATGTGAA GCGGCTGTGC TTGAAGGCAA AGGAGATTTT CACGAGTGAGGAGAACGTGC ACAAGATCCC GGCTCCGTGC ACCATCGTGG GCGACATCCA TGGCCAATTCTACGACCTCC TGGAGCTCTT CCGCGTCGGT GGCGAAATCC CGGACACAAA CTACGTCTTCATGGGCGATT TCGTAGACCG AGGCTACCAT AGCGTAGAGT CGTTCCTGTT GCTACTTGTGCTCAAGATCA AGTACTCTCG TCGCGTTGCG CTGGTGCGCG GCAACCACGA GTCGCGCCAAATTACGCAGG TGTACGGCTT CTACGATGAG TGCTTGCGCA AGTACGGCAG CATCAACGTGTGGCGCTACT GCACGGACGT GTTCGATCTC TTGCCCCTAG CGAGCGTCGT GGAAGGGAAGATCTTCTGCG TGCACGCTGG TCTGTCGCCA TCGATACAGA CGGTGGACCA GATGCGTTCCATCCGCCGTA ACTGCGAGGT GCCGCACGAA GGCGCCATGT GCGACTTGCT CTGGTCCGATCCGGAGGACA TTGACGGCTG GGGTCTGTCG CCGCGCGGCG CCGGCTACCT GTTCGGCGGTGATGTCGTGT GCCAGTTCAA CGAGACAAAC AAGCTAGACC TCATCTGCCG CTCCCATCAGCTGGTGATGG AGGGATACAA GGCTATGTTC AATGACACGC TTGTGACGGT CTGGTCGGCGCCCAACTACT GTTACCGCTG TGGTAACGTT GCGTCTATTT TGGAGCTGGA CGAGCACCTGAACAAGAACT TCAAGATTTT TGAGGCTGCG CCGGCAGAGG CGCGCGAGGC TGGTCAGCGGAACGAGGTAC CAGCCTACTT CCTTTAG |
| Serine/threonine protein phosphatase Q4Q4W8] | |
|---|---|
| Metabolite Information | phosphate metabolism |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K01090 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | protein phosphatase 4 (formerly X), catalytic subunit [Homo sapiens] | 68 | 1e-132 | 466 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YER133w serthr phosphoprotein phosphatase 1, catalytic chain | Saccharomyces cerevisiae | 45% | 2e-74 | 273 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 227-230; 251-254; | PS00722 | |
| PDOC00115 | Serine/threonine specific protein phosphatases signature | 112-117; | PS00125 | |
| Acylation | N-myristoylation site | 114-119; 167-172; 268-273; | PS00008 | |
| Phosphorylation | Casein kinase II phosphorylation site | 38-41; 155-158; 199-202; 272-275; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 106-108; 180-182; 210-212; 229-231; | PS00005 | |
| Serine/threonine protein phosphatase [Q4Q4W8] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2nppF | |
| Percent Identity | 59% | |
| Target Region | 1-308 | |
| Template Region | 2-308 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 86 | Sidechain |
| ARG | 87 | Sidechain |
| ASN | 115 | Sidechain |
| HIS | 116 | Sidechain |
| ARG | 212 | Sidechain |
| HIS | 239 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 16397-91-4 | MANGANESE (II) ION | 54.938 | Mn | [Mn+2] | 2npp |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/518397/Q4Q4W8.pdb 2.0 308 = residues | | = | *| Ramachandran plot: 87.9% core 10.6% allow 0.7% gener 0.7% = disall | | = | +| All Ramachandrans: 9 labelled residues (out of 306) = | +| Chi1-chi2 plots: 3 labelled residues (out of 194) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 3 | *| Bond len/angle: 12.1 Morris et al class: 1 = 1 2 | +| 2 cis-peptides = | | G-factors Dihedrals: -0.01 Covalent: -0.16 Overall: = -0.06 | | = | | M/c bond lengths: 99.7% within limits 0.3% highlighted = | *| M/c bond angles: 94.1% within limits 5.9% highlighted 1 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |