| Beta-tubulin [Q4Q4C4] | |
|---|---|
| Systematic Name | LmjF.33.0792 [Leishmania major] |
| Gene Name | LMJF_33_0792 |
| Molecular Weight | 49740 Da |
| Protein Sequence Size | 443 |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a nonexchangeable site on the alpha-chain |
| Charge | -21 |
| Isoelectric Point | 4.4459 pH |
| Description | Beta-tubulin. |
| Subcellular Location | microtubule; protein complex[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4Q4C4|Q4Q4C4_LEIMA Beta-tubulin - Leishmania major REIVSCQAGQCGNQIGSKFWEVIADEHGVDPTGSYQGDSDLQLERINVYFDESAGGRYVP RAVLMDLEPGTMDSVRAGPYGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELIDSVLDVCR KEAESCDCLQGFQLSHSLGGGTGSGMGTLLISKLREEYPDRIMMTFSVIPSPRVSDTVVE PYNTTLSVHQLVENSDESMCIDNEALYDICFRTLKLTTPTFGDLNHLVAAVMSGVTCCLR FPGQLNSDLRKLAVNLVPFPRLHFFMMGFAPLTSRGSQQYRGLSVAELTQQMFDAKNMMQ AADPRHGRYLTASALFRGRMSTKEVDEQMLNVQNKNSSYFIEWIPNNIKSSICDIPPKGL KMSVTFIGNNTCIQEMFRRVGEQFTGMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSE YQQYQDATVEEEGEYDEEEEAY |
| DNA Sequence | >LmjF33.0792 |||beta-tubulin|Leishmania major|chr 33|||Manual ATGCGTGAGA TCGTTTCCTG CCAGGCCGGC CAGTGCGGCA ACCAGATCGG CTCTAAGTTTTGGGAGGTGA TTGCCGACGA ACATGGTGTC GATCCGACTG GCTCCTACCA GGGTGACTCGGATCTGCAGC TGGAGCGCAT CAACGTGTAC TTCGATGAGT CGGCGGGAGG CCGCTACGTGCCGCGCGCCG TGCTGATGGA CCTCGAGCCC GGCACTATGG ACTCCGTTCG CGCCGGCCCGTACGGCCAGC TGTTCCGCCC GGACAACTTC ATCTTTGGTC AGTCCGGCGC TGGCAACAACTGGGCCAAGG GCCACTACAC TGAGGGTGCG GAGCTGATCG ACTCCGTGCT TGATGTGTGCCGCAAGGAGG CGGAGAGCTG CGACTGCCTG CAGGGCTTCC AGCTGTCTCA CTCCCTCGGCGGCGGCACGG GCTCCGGCAT GGGCACGCTG CTCATTTCCA AGCTGCGCGA GGAGTACCCGGACCGGATCA TGATGACCTT CTCCGTCATC CCGTCCCCCC GCGTGTCGGA TACCGTTGTGGAGCCGTACA ACACGACCCT CTCTGTGCAC CAGCTCGTGG AGAACTCCGA CGAGTCCATGTGCATCGACA ACGAGGCGCT GTACGATATT TGCTTCCGCA CGCTGAAGCT GACGACGCCGACGTTCGGTG ACCTGAACCA CCTCGTCGCC GCTGTGATGT CTGGCGTGAC CTGCTGCCTGCGCTTCCCTG GCCAGCTGAA CTCTGACCTG CGCAAGCTTG CCGTGAACCT CGTGCCGTTCCCGCGCCTGC ACTTCTTCAT GATGGGCTTC GCGCCGCTGA CGAGCCGCGG CTCGCAGCAGTACCGCGGCC TGTCCGTCGC GGAGCTGACG CAGCAGATGT TCGACGCCAA GAACATGATGCAGGCCGCCG ACCCGCGCCA CGGCCGCTAC CTCACCGCGT CCGCGCTGTT CCGCGGCCGCATGTCGACCA AGGAGGTGGA CGAGCAGATG CTGAACGTGC AGAACAAGAA CTCCAGCTACTTCATCGAGT GGATCCCGAA CAACATCAAG TCGTCCATCT GCGATATCCC GCCCAAGGGTCTCAAGATGT CTGTCACCTT CATCGGCAAC AACACCTGCA TCCAGGAGAT GTTCCGCCGCGTCGGTGAGC AGTTCACGGG TATGTTCCGC CGCAAGGCCT TCCTCCACTG GTACACCGGTGAGGGCATGG ACGAGATGGA GTTCACGGAG GCCGAGTCCA ACATGAACGA CCTCGTCTCTGAGTACCAGC AGTACCAGGA CGCCACCGTC GAGGAGGAGG GCGAGTACGA CGAGGAGGAGGAAGCCTACT AG |
| Beta-tubulin Q4Q4C4] | |
|---|---|
| Metabolite Information | cell motility; microtubule cytoskeleton organization and biogenesis; microtubule-based movement; protein polymerization |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K07375 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | tubulin, beta, 2 [Homo sapiens] | 82 | 0 | 751 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YFL037w beta-tubulin | Saccharomyces cerevisiae | 68% | 0 | 630 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 184-187; 337-340; 370-373; | PS00722 | |
| PDOC00199 | Tubulin subunits alpha, beta, and gamma signature | 140-146; | PS00227 | |
| PDOC00200 | Tubulin-beta mRNA autoregulation signal | 1-4; | PS00228 | |
| Acylation | N-myristoylation site | 10-15; 13-18; 29-34; 34-39; 71-76; 93-98; 96-101; 98-103; 140-145; 141-146; 142-147; 144-149; 235-240; 244-249; 283-288; 360-365; 369-374; | PS00008 | |
| Glycosylation | N-glycosylation site | 184-187; 337-340; 370-373; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 115-118; 178-181; 221-224; 285-288; 322-325; 352-355; 409-412; 429-432; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 75-77; 172-174; 214-216; 274-276; 322-324; | PS00005 | |
| Sulfation | Tyrosine sulfation site | 43-57; 415-429; | PS00003 | |
| Beta-tubulin [Q4Q4C4] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2hxhB | |
| Percent Identity | 82% | |
| Target Region | 1-443 | |
| Template Region | 2-426 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 58-64-0 | ADENOSINE-5'-DIPHOSPHATE | 427.201 | C10 H15 N5 O10 P2 | O=P(O)(O)OP(=O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O | 2hxh |
| 146-91-8 | GUANOSINE-5'-DIPHOSPHATE | 443.201 | C10 H15 N5 O11 P2 | O=P(O)(O)OP(=O)(O)OCC3OC(n2cnc1c2N=C(N)NC1=O)C(O)C3O | 2hxh |
| 86-01-1 | GUANOSINE-5'-TRIPHOSPHATE | 523.18 | C10 H16 N5 O14 P3 | O=P(O)(O)OP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c2N=C(N)NC1=O)C(O)C3O | 2hxh |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 2hxh |
| TAXOL | 853.906 | C47 H51 N O14 | O=C(c1ccccc1)NC(c2ccccc2)C(O)C(=O)OC5C(=C4C(OC(=O)C)C(=O)C7(C(C(OC(=O)c3ccccc3)C(O)(C4(C)C)C5)C6(OC(=O)C)C(OC6)CC7O)C)C | 2hxh | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1793694/Q4Q4C4.pdb 2.0 443 = residues | | = | *| Ramachandran plot: 88.6% core 9.4% allow 1.3% gener 0.8% = disall | | = | *| All Ramachandrans: 23 labelled residues (out of 441) = | +| Chi1-chi2 plots: 2 labelled residues (out of 268) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 12.2 Bad contacts: = 15 | *| Bond len/angle: 7.0 Morris et al class: 1 = 1 3 | | = | | G-factors Dihedrals: -0.10 Covalent: -0.18 Overall: = -0.13 | | = | | M/c bond lengths: 99.1% within limits 0.9% highlighted = | | M/c bond angles: 93.0% within limits 7.0% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |