| Isocitrate dehydrogenase, putative [Q4Q3T2] | |
|---|---|
| Systematic Name | LmjF.33.2550 [Leishmania major] |
| Gene Name | LMJF_33_2550 |
| Molecular Weight | 46335 Da |
| Protein Sequence Size | 425 |
| Function | |
| Charge | -7.5 |
| Isoelectric Point | 5.2057 pH |
| Description | Isocitrate dehydrogenase, putative (EC 1.1.1.42). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 1.1.1.42 |
| Sequence | >tr|Q4Q3T2|Q4Q3T2_LEIMA Isocitrate dehydrogenase, putative (EC 1.1.1.42) - Leishmania major. AESISFAKGALVVPSCPIIPYIEGDGIGKEITEVAHRVFDAAVAKEYGTTRKISWLEVLA GEKAFEQKGSWLPEETIEAFDKHRIGMKGPLTTPVGEGIRSLNVVLRHRLDLFVCQRPVR WFTGVGSPIRHPEWVDMVVFRENTEDIYSGIEWGQGTPEAAKFADFLTKEMGVTTVRFPE TSAYGVKPVSLEGSERLVRAAVQYAIDHHLPSVTLVHKGNIMKFTEGCFKKWGYALAERE FPDAAFTMTQYDAIKEKGGKEAAETALREAMDSGKVVIKDCICDAFLQNTILRPLDYSVI ATMNLNGDYVSDQLAALVGGIGIAPGANINYVTGHAIFEATHGTAPDIAGQGKANPSSLI LSGAMMFDYMGWSEVSARIINALEMVFQANMATVDLARQMTGARALSTAEFGEAIIKKIN GDEA |
| DNA Sequence | >LmjF33.2550 |||isocitrate dehydrogenase, putative|Leishmania major|chr 33|||Manual ATGGCGGAAT CCATCTCTTT CGCGAAGGGG GCGCTGGTGG TGCCAAGTTG CCCCATTATCCCCTACATTG AGGGGGACGG CATTGGCAAG GAGATCACGG AGGTGGCGCA CCGCGTCTTTGATGCGGCGG TGGCAAAGGA GTACGGCACC ACCCGGAAGA TTTCGTGGCT AGAGGTGCTCGCGGGGGAGA AAGCGTTCGA GCAGAAGGGC AGCTGGCTGC CGGAGGAGAC CATCGAGGCATTTGACAAGC ACCGCATTGG CATGAAGGGC CCACTGACAA CCCCGGTTGG TGAGGGGATCCGCTCGCTCA ACGTGGTGCT GCGTCACCGG CTGGACTTGT TCGTCTGCCA GCGCCCCGTACGATGGTTCA CCGGCGTCGG ATCGCCGATC CGACACCCGG AGTGGGTGGA CATGGTTGTGTTTCGCGAGA ACACGGAGGA CATATACTCT GGCATTGAGT GGGGCCAGGG CACCCCTGAGGCTGCCAAAT TTGCAGACTT TCTGACGAAG GAGATGGGTG TGACGACGGT GCGCTTCCCTGAGACGAGCG CGTACGGCGT GAAGCCGGTT TCGCTGGAGG GAAGCGAGCG GCTGGTGCGTGCGGCGGTGC AGTACGCTAT CGATCACCAT CTTCCGTCTG TTACGCTGGT GCACAAAGGCAACATCATGA AGTTCACAGA GGGTTGCTTC AAGAAGTGGG GCTACGCCTT GGCCGAGCGTGAGTTCCCCG ATGCCGCCTT CACCATGACG CAGTACGACG CCATCAAGGA GAAAGGCGGCAAGGAGGCGG CTGAGACGGC GCTGCGCGAG GCGATGGACA GCGGCAAAGT CGTTATCAAGGATTGCATCT GTGACGCCTT TCTGCAAAAC ACGATTCTCC GCCCATTGGA CTATAGCGTGATTGCCACCA TGAACCTGAA CGGCGATTAC GTGTCTGATC AGCTGGCCGC GCTTGTCGGTGGCATCGGCA TCGCGCCAGG CGCGAACATC AACTATGTGA CCGGCCACGC TATTTTCGAGGCGACGCATG GGACGGCGCC GGATATCGCT GGGCAAGGTA AAGCGAACCC ATCCTCGCTCATCCTATCTG GGGCTATGAT GTTTGACTAC ATGGGCTGGT CCGAGGTGTC GGCGCGCATCATCAACGCGC TTGAGATGGT CTTTCAGGCA AACATGGCAA CCGTCGATCT TGCGCGGCAGATGACCGGTG CCAGGGCACT CTCGACAGCA GAATTCGGGG AGGCGATCAT CAAGAAGATCAACGGCGACG AGGCGTAG |
| Isocitrate dehydrogenase, putative Q4Q3T2] | |
|---|---|
| Metabolite Information | metabolism; tricarboxylic acid cycle |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K00031 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | isocitrate dehydrogenase 3 (NAD+) alpha precursor [Homo sapiens] | 30 | 2e-36 | 150 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| icdA isocitrate dehydrogenase | Salmonella typhimurium | 51% | 1e-112 | 399 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00389 | Isocitrate and isopropylmalate dehydrogenases signature | 305-324; | PS00470 | |
| Acylation | N-myristoylation site | 151-156; 157-162; 259-264; 323-328; 327-332; 351-356; | PS00008 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 52-55; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 55-58; 150-153; 250-253; 345-348; 408-411; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 50-52; 51-53; 176-178; 195-197; 274-276; 377-379; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 142-149; 178-185; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 142-156; | PS00003 | |
| Isocitrate dehydrogenase, putative [Q4Q3T2] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1hqsB | |
| Percent Identity | 55% | |
| Target Region | 1-425 | |
| Template Region | 1-422 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| LYS | 219 | Sidechain |
| ASP | 285 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Mn | Binds 1 magnesium or manganese ion per subunit |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 5949-29-1 | CITRIC ACID | 192.124 | C6 H8 O7 | O=C(O)CC(O)(C(=O)O)CC(=O)O | 1hqs |
| S-1,2-PROPANEDIOL | 76.094 | C3 H8 O2 | OCC(O)C | 1hqs | |
| R-1,2-PROPANEDIOL | 76.094 | C3 H8 O2 | OCC(O)C | 1hqs | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/2235540/Q4Q3T2.pdb 2.0 425 = residues | | = | *| Ramachandran plot: 92.9% core 5.5% allow 1.1% gener 0.5% = disall | | = | +| All Ramachandrans: 7 labelled residues (out of 423) = | +| Chi1-chi2 plots: 2 labelled residues (out of 236) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 0 | *| Bond len/angle: 7.9 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.10 Covalent: -0.09 Overall: = 0.03 | | = | | M/c bond lengths: 99.7% within limits 0.3% highlighted = | | M/c bond angles: 95.2% within limits 4.8% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |