| Glucose-6-phosphate 1-dehydrogenase [Q4Q3K1] | |
|---|---|
| Systematic Name | LmjF.34.0080 [Leishmania major] |
| Gene Name | G6PD |
| Molecular Weight | 63341 Da |
| Protein Sequence Size | 562 |
| Function | |
| Charge | -5.5 |
| Isoelectric Point | 5.7141 pH |
| Description | Glucose-6-phosphate 1-dehydrogenase (G6PD). |
| Subcellular Location | cytoplasm; glycosome[Predict] |
| E. C. Number | 1.1.1.49 |
| Sequence | >tr|Q4Q3K1|Q4Q3K1_LEIMA Glucose-6-phosphate 1-dehydrogenase (G6PD) - Leishmania major. SEEQSHADQDAYVADVDGILDVLRAEVLERKPDDVFQFISQSALSLQKDRGAESCDRINC KVTDEQKSRALTIIVFGASGDLAKKKTFPALFDLYCGGLLPPEVNVIGYARTKVDDAERW KRETLMTYFSNVPERACHAEDFLKHISYFCGSYDKVDDFKRLDAVIREKENAFKGPEKGG NRLFYLALPPSVFASVCESIHKGAMPQEVGGWARVIIEKPFGRDTKSSAELSRALEPFFD ESQLYRIDHYLGKEMVQNIITTRFANRIFSAVWNSNNIACVQITFKETIGTEGRGGYFDG IGIIRDVMQNHLTQILALLAMEKPRSLDAECIRDEKVSVLKCIEPITKENCVLGQYTASA DGSIPGYQEDVTVPEGSTCPTFAVMRLNINNDRWAGVPFILKAGKAVEQKYVAIRIQFKD EVHPYGEATQRNELVIRAQPSEAMYVKITTKVPGLSGDLRQTHQTELDLTYHTRYDVRLP DAYESLINDALLGNSTNFVRKDELDVAWRIFTPLLHQIDCGEIKPIPYQAGTRGPKEADE FITNNGFKHQKGYQWLPSNKL |
| DNA Sequence | >LmjF34.0080 |G6PD||glucose-6-phosphate dehydrogenase|Leishmania major|chr 34|||Manual ATGTCGGAAG AGCAGTCTCA TGCTGATCAG GATGCCTACG TGGCCGATGT CGACGGCATTCTGGACGTGC TCCGTGCGGA AGTGCTGGAG CGGAAGCCGG ATGACGTTTT CCAGTTCATCTCCCAGTCGG CGCTTAGTCT GCAGAAGGAC AGGGGCGCCG AATCGTGCGA TCGCATCAACTGTAAGGTCA CGGATGAACA GAAGAGCCGT GCGTTGACTA TCATCGTGTT CGGTGCCAGCGGGGATCTGG CCAAGAAGAA GACATTTCCT GCCCTCTTCG ATCTGTACTG CGGCGGGCTCCTCCCCCCGG AGGTCAACGT TATCGGCTAT GCTCGCACTA AGGTGGACGA TGCCGAGAGGTGGAAGCGTG AGACGCTCAT GACGTATTTT TCGAACGTGC CGGAGCGCGC GTGCCACGCCGAGGACTTCT TGAAACATAT CAGCTACTTC TGTGGATCGT ACGACAAGGT GGACGACTTCAAGCGTCTTG ACGCGGTGAT TCGCGAGAAG GAGAATGCCT TTAAGGGCCC TGAGAAGGGTGGAAACCGTC TCTTCTACCT GGCTCTTCCG CCATCGGTAT TCGCAAGTGT CTGCGAGAGCATTCACAAGG GTGCGATGCC GCAGGAAGTG GGGGGATGGG CGCGGGTGAT CATCGAGAAGCCCTTTGGCC GCGATACCAA GAGCTCCGCC GAGCTGTCCC GAGCGCTGGA GCCGTTCTTCGACGAGTCGC AGCTGTACCG CATTGACCAC TACCTCGGGA AGGAGATGGT GCAGAACATCATCACGACAC GCTTCGCCAA CCGTATCTTC AGCGCTGTGT GGAACTCGAA CAACATCGCGTGCGTGCAGA TCACGTTCAA GGAGACCATC GGCACTGAGG GCCGTGGCGG CTACTTCGACGGCATCGGCA TTATTCGCGA CGTCATGCAA AACCATCTCA CCCAGATTCT TGCCCTGCTGGCCATGGAGA AGCCTCGGTC GCTGGACGCT GAGTGCATCC GCGACGAGAA GGTGTCGGTGCTGAAGTGCA TTGAGCCAAT AACGAAGGAG AATTGTGTTC TGGGCCAGTA CACTGCCTCCGCCGATGGTT CCATCCCTGG TTACCAAGAA GACGTGACCG TACCCGAGGG CAGCACTTGCCCCACATTTG CCGTGATGCG GCTCAACATC AACAACGACC GCTGGGCCGG CGTCCCTTTTATTCTCAAGG CCGGCAAGGC TGTGGAGCAG AAGTACGTGG CGATTCGCAT TCAGTTCAAGGATGAGGTCC ACCCCTACGG CGAGGCGACA CAGCGCAACG AGCTCGTCAT CCGTGCCCAGCCGTCCGAGG CCATGTATGT CAAGATCACC ACAAAGGTTC CTGGCCTTAG TGGGGACTTGCGGCAGACGC ACCAGACAGA GCTGGACCTC ACATACCACA CCCGGTACGA TGTACGCCTTCCAGACGCCT ACGAGAGCCT CATCAACGAC GCACTGCTCG GCAACTCGAC AAACTTTGTGCGTAAGGACG AGCTCGATGT GGCATGGCGC ATCTTCACGC CGCTCCTGCA CCAGATCGACTGTGGAGAGA TCAAACCGAT TCCGTACCAG GCCGGCACGC GCGGGCCCAA GGAAGCGGATGAATTCATCA CCAACAACGG CTTCAAGCAC CAGAAAGGCT ACCAATGGCT TCCCTCGAATAAGCTGTGA |
| Glucose-6-phosphate 1-dehydrogenase Q4Q3K1] | |
|---|---|
| Metabolite Information | glucose metabolism; pentose-phosphate shunt |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K00036 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | glucose-6-phosphate dehydrogenase [Homo sapiens] | 51 | 1e-136 | 482 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| zwf Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) | Escherichia coli MG1655 | 38% | 8e-84 | 305 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 495-498; | PS00722 | |
| PDOC00067 | Glucose-6-phosphate dehydrogenase active site | 249-255; | PS00069 | |
| PDOC00299 | Microbodies C-terminal targeting signal | 560-562; | PS00342 | |
| Acylation | N-myristoylation site | 52-57; 355-360; 363-368; 494-499; | PS00008 | |
| Glycosylation | N-glycosylation site | 495-498; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 85-88; 122-125; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 6-9; 113-116; 196-199; 228-231; 285-288; 373-376; 466-469; 474-477; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 262-264; 285-287; 430-432; 450-452; 559-561; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 406-412; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 6-20; 147-161; 419-433; | PS00003 | |
| Glucose-6-phosphate 1-dehydrogenase [Q4Q3K1] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2bhlB | |
| Percent Identity | 51% | |
| Target Region | 67-562 | |
| Template Region | 27-479 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 183 | Sidechain |
| HIS | 246 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 56-73-5 | BETA-D-GLUCOSE-6-PHOSPHATE | 260.136 | C6 H13 O9 P | O=P(O)(O)OCC1OC(O)C(O)C(O)C1O | 2bhl |
| 8013-25-0 | GLYCEROL | 92.094 | C3 H8 O3 | OCC(O)CO | 2bhl |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
|
|
|
| Modeled Protein | Template Structure |
|
|
+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/3813705/Q4Q3K1.pdb 2.0 496 = residues | | = | +| Ramachandran plot: 91.7% core 7.6% allow 0.7% gener 0.0% = disall | | = | +| All Ramachandrans: 8 labelled residues (out of 494) = | +| Chi1-chi2 plots: 4 labelled residues (out of 306) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 6.6 Bad contacts: = 6 | *| Bond len/angle: 7.0 Morris et al class: 1 = 1 2 | +| 2 cis-peptides = | | G-factors Dihedrals: 0.03 Covalent: -0.14 Overall: = -0.04 | | = | | M/c bond lengths: 99.2% within limits 0.8% highlighted = | | M/c bond angles: 94.2% within limits 5.8% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |