| Malate dehydrogenase [Q4Q3J4] | |
|---|---|
| Systematic Name | LmjF.34.0140 [Leishmania major] |
| Gene Name | LMJF_34_0140 |
| Molecular Weight | 33358 Da |
| Protein Sequence Size | 317 |
| Function | |
| Charge | 5 |
| Isoelectric Point | 8.4533 pH |
| Description | Malate dehydrogenase (EC 1.1.1.37). |
| Subcellular Location | Mitochondrion[Predict] |
| E. C. Number | 1.1.1.37 |
| Sequence | >tr|Q4Q3J4|Q4Q3J4_LEIMA Malate dehydrogenase (EC 1.1.1.37) - Leishmania major. RRSQGCFFRVAVLGAAGGIGQPLSLLLKNNKYVKELKLYDVKGGPGVAADLSHICAPAKV TGYTKDELSRAVENADVVVIPAGIPRKPGMTRDDLFNTNASIVRDLAIAVGTHAPKAIVG IITNPVNSTVPVAAEALKKVGVYDPARLFGVTTLDVVRARTFVAEALGASPYDVDVPVIG GHSGETIVPLLSGFPSLSEEQVRQLTHRIQFGGDEVVKAKDGAGSATLSMAFAGNEWTTA VLRALSGEKGVVVCTYVQSTVEPSCAFFSSPVLLGNSGVEKIYPVPMLNAYEEKLMAKCL EGLQSNITKGIAFSNK |
| DNA Sequence | >LmjF34.0140 |||malate dehydrogenase|Leishmania major|chr 34|||Manual ATGCGCCGTT CTCAGGGGTG CTTCTTCCGT GTCGCTGTGC TCGGTGCTGC CGGTGGCATCGGCCAGCCCT TGTCGCTCCT CCTCAAAAAT AACAAGTACG TGAAGGAGCT GAAGCTGTACGATGTCAAGG GTGGACCAGG CGTGGCTGCG GACCTCTCCC ACATCTGCGC TCCAGCGAAGGTGACTGGGT ACACGAAGGA TGAGCTCTCA AGAGCTGTCG AGAACGCTGA CGTTGTGGTGATCCCCGCTG GCATCCCCCG CAAACCGGGG ATGACGCGCG ACGACCTCTT TAACACCAACGCCAGCATTG TCCGAGATCT CGCCATCGCC GTTGGCACGC ACGCACCGAA GGCAATTGTTGGCATTATCA CGAACCCCGT CAACAGCACT GTGCCAGTGG CTGCTGAGGC GCTGAAGAAGGTCGGCGTGT ACGATCCTGC GCGCCTCTTT GGTGTCACCA CACTCGACGT TGTCCGTGCCCGCACCTTCG TCGCGGAGGC GCTCGGTGCC AGCCCGTACG ACGTTGACGT CCCTGTTATTGGCGGCCACA GCGGTGAGAC GATTGTGCCG TTGCTCTCGG GCTTCCCGTC GCTGTCGGAGGAGCAGGTGC GGCAGCTGAC GCACCGCATC CAGTTTGGTG GTGATGAGGT GGTGAAGGCCAAGGATGGTG CGGGCTCGGC GACGCTGTCC ATGGCGTTCG CAGGCAACGA GTGGACGACGGCGGTGCTGC GCGCCCTCAG TGGCGAGAAG GGTGTCGTGG TGTGCACGTA CGTGCAGAGCACCGTGGAGC CGTCGTGCGC CTTCTTCAGC TCTCCGGTGT TGCTGGGCAA CAGTGGTGTGGAAAAGATCT ACCCTGTGCC GATGCTGAAC GCATACGAAG AGAAGCTGAT GGCCAAGTGCTTGGAGGGTC TGCAGAGCAA CATCACGAAG GGCATCGCGT TCAGCAACAA GTAA |
| Malate dehydrogenase Q4Q3J4] | |
|---|---|
| Metabolite Information | malate metabolism; tricarboxylic acid cycle; tricarboxylic acid cycle intermediate metabolism |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K00026 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | mitochondrial malate dehydrogenase precursor [Homo sapiens] | 54 | 7e-89 | 324 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| mdh malate dehydrogenase | Haemophilus influenzae | 52% | 9e-76 | 277 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 100-103; 128-131; 307-310; | PS00722 | |
| PDOC00066 | Malate dehydrogenase active site signature | 152-164; | PS00068 | |
| Acylation | N-myristoylation site | 15-20; 21-26; 181-186; 223-228; 235-240; 251-256; 303-308; 311-316; | PS00008 | |
| Glycosylation | N-glycosylation site | 100-103; 128-131; 307-310; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 65-68; 92-95; 153-156; 171-174; 197-200; 260-263; 278-281; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 207-209; 315-317; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 32-40; | PS00007 | |
| Malate dehydrogenase [Q4Q3J4] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2dfdD | |
| Percent Identity | 54% | |
| Target Region | 1-317 | |
| Template Region | 6-314 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 156 | Sidechain |
| ARG | 159 | Sidechain |
| HIS | 183 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 6898-94-8 | ALANINE | 89.093 | C3 H7 N O2 | O=C(O)C(N)C | 2dfd |
| 16887-00-6 | CHLORIDE ION | 35.453 | Cl | [Cl-] | 2dfd |
| 07-02-6027 00:00:00 | HISTIDINE | 156.162 | C6 H10 N3 O2 | O=C(O)C(N)Cc1cnc[nH+]1 | 2dfd |
| MALATE ION | 133.079 | C4 H5 O5 | [O-]C(=O)C(O)CC(=O)O | 2dfd | |
| NICOTINAMIDE-ADENINE-DINUCLEOTIDE | 663.425 | C21 H27 N7 O14 P2 | c1cc(c[n+](c1)C2C(C(C(O2)COP(=O)([O-])OP(=O)(O)OCC3C(C(C(O3)n4cnc5c4ncnc5N)O)O)O)O)C(=O)N | 2dfd | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/2720256/Q4Q3J4.pdb 2.0 317 = residues | | = | +| Ramachandran plot: 94.7% core 4.5% allow 0.8% gener 0.0% = disall | | = | +| All Ramachandrans: 8 labelled residues (out of 315) = | +| Chi1-chi2 plots: 1 labelled residues (out of 152) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 9.4 Bad contacts: = 6 | *| Bond len/angle: 6.2 Morris et al class: 1 = 1 2 | +| 2 cis-peptides = | | G-factors Dihedrals: 0.09 Covalent: -0.14 Overall: = 0.00 | | = | | M/c bond lengths: 99.4% within limits 0.6% highlighted = | *| M/c bond angles: 93.6% within limits 6.4% highlighted 1 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |