| Malate dehydrogenase, putative [Q4Q3J3] | |
|---|---|
| Systematic Name | LmjF.34.0150 [Leishmania major] |
| Gene Name | LMJF_34_0150 |
| Molecular Weight | 35931 Da |
| Protein Sequence Size | 331 |
| Function | |
| Charge | 3 |
| Isoelectric Point | 7.4 pH |
| Description | Malate dehydrogenase, putative (EC 1.1.1.37). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 1.1.1.37 |
| Sequence | >tr|Q4Q3J3|Q4Q3J3_LEIMA Malate dehydrogenase, putative (EC 1.1.1.37) - Leishmania major. KPSTLSRFKVTVLGASGAIGQPLALALVQNKRVSELALYDIVQPRGVAVDLSHFPRKVKV TGYPTKWIHKALDGADLVLMSAGMPRRPGMTHDDLFNTNALTVNELSAAVARYAPKSVLA IISNPLNSMVPVAAETLQRAGVYDPRKLFGIISLNMMRARKMLGDFTGQDPEMLDVPVIG GHSGQTIVPLFSHSGVELRQEQVEYLTHRVRVGGDEVVKAKEGRGSSSLSMAFAAAEWAD GVLRAMDGEKTLLQCSFVESPLFADKCRFFGSTVEVCKEGIERVLPLPPLNEYEEEQLDR CLPDLEKNIRKGLAFVAENAATSTPSTASP |
| DNA Sequence | >LmjF34.0150 |||malate dehydrogenase, putative|Leishmania major|chr 34|||Manual ATGAAACCAT CGACCTTAAG TCGCTTCAAA GTCACCGTCT TGGGCGCCAG CGGGGCCATCGGCCAGCCGC TCGCTCTGGC GCTCGTACAG AACAAGCGAG TCAGTGAGCT GGCACTCTACGACATTGTGC AGCCGCGCGG CGTCGCCGTC GACCTCTCCC ATTTTCCACG CAAGGTCAAGGTAACGGGGT ACCCGACAAA GTGGATTCAC AAGGCTCTAG ATGGAGCAGA TCTCGTTCTGATGTCGGCTG GCATGCCCCG CCGACCCGGC ATGACCCACG ATGACCTCTT CAACACAAATGCTCTGACTG TGAACGAGCT GAGCGCGGCG GTGGCGAGGT ACGCCCCTAA ATCCGTTTTAGCCATTATTA GCAATCCACT GAATAGCATG GTGCCTGTCG CCGCAGAGAC GCTGCAGCGGGCTGGAGTGT ATGACCCTCG CAAGCTCTTT GGTATCATCT CGCTGAACAT GATGCGGGCACGCAAGATGC TGGGCGATTT TACTGGTCAG GACCCGGAGA TGCTGGACGT GCCCGTCATTGGCGGGCACA GCGGGCAAAC CATAGTTCCT CTTTTCTCGC ACTCTGGAGT GGAGTTGAGGCAAGAGCAGG TGGAGTACCT GACGCATCGC GTACGCGTGG GTGGCGACGA GGTGGTGAAGGCGAAGGAGG GCCGTGGGTC TTCGTCGCTG TCGATGGCGT TTGCGGCTGC CGAGTGGGCGGACGGGGTGC TGCGTGCGAT GGACGGCGAG AAGACTTTAT TGCAGTGCTC TTTTGTCGAAAGCCCGCTGT TTGCTGACAA GTGCCGCTTC TTCGGTAGCA CCGTGGAGGT ATGCAAGGAGGGAATCGAGC GGGTGCTGCC ACTGCCTCCG CTGAACGAGT ATGAAGAGGA GCAGCTGGACCGCTGCCTCC CGGACCTGGA GAAGAACATC CGCAAAGGAT TGGCATTCGT GGCGGAAAACGCGGCCACCT CGACACCCTC GACCGCCTCG CCTTAG |
| Malate dehydrogenase, putative Q4Q3J3] | |
|---|---|
| Metabolite Information | malate metabolism; tricarboxylic acid cycle intermediate metabolism |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K00016 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | mitochondrial malate dehydrogenase precursor [Homo sapiens] | 43 | 2e-69 | 259 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| mdh malate dehydrogenase | Haemophilus influenzae | 43% | 8e-61 | 228 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00015 | The rule PS00015 has been deleted from the PROSITE database and replaced by the following motif: PS50079 (Bipartite nuclear localization signal profile | 147-163; | PS00015 | |
| Acylation | N-myristoylation site | 15-20; 21-26; 181-186; 242-247; | PS00008 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 32-35; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 92-95; 103-106; 168-171; 195-198; 257-260; 273-276; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 208-210; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 32-40; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 287-301; | PS00003 | |
| Malate dehydrogenase, putative [Q4Q3J3] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2dfdD | |
| Percent Identity | 43% | |
| Target Region | 8-323 | |
| Template Region | 6-314 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASN | 149 | Sidechain (ASP 155 In Template) |
| ARG | 152 | Sidechain |
| HIS | 176 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 6898-94-8 | ALANINE | 89.093 | C3 H7 N O2 | O=C(O)C(N)C | 2dfd |
| 16887-00-6 | CHLORIDE ION | 35.453 | Cl | [Cl-] | 2dfd |
| 07-02-6027 00:00:00 | HISTIDINE | 156.162 | C6 H10 N3 O2 | O=C(O)C(N)Cc1cnc[nH+]1 | 2dfd |
| MALATE ION | 133.079 | C4 H5 O5 | [O-]C(=O)C(O)CC(=O)O | 2dfd | |
| NICOTINAMIDE-ADENINE-DINUCLEOTIDE | 663.425 | C21 H27 N7 O14 P2 | c1cc(c[n+](c1)C2C(C(C(O2)COP(=O)([O-])OP(=O)(O)OCC3C(C(C(O3)n4cnc5c4ncnc5N)O)O)O)O)C(=O)N | 2dfd | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1062957/Q4Q3J3.pdb 2.0 313 = residues | | = | | Ramachandran plot: 95.5% core 4.5% allow 0.0% gener 0.0% = disall | | = | +| All Ramachandrans: 8 labelled residues (out of 311) = | +| Chi1-chi2 plots: 3 labelled residues (out of 175) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 9.3 Bad contacts: = 4 | *| Bond len/angle: 10.1 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.07 Covalent: -0.15 Overall: = -0.01 | | = | | M/c bond lengths: 99.4% within limits 0.6% highlighted = | *| M/c bond angles: 94.0% within limits 6.0% highlighted 2 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |