| Malate dehydrogenase, putative [Q4Q3J2] | |
|---|---|
| Systematic Name | LmjF.34.0160 [Leishmania major] |
| Gene Name | mMDH |
| Molecular Weight | 34145 Da |
| Protein Sequence Size | 325 |
| Function | |
| Charge | 1 |
| Isoelectric Point | 6.7215 pH |
| Description | Malate dehydrogenase, putative (EC 1.1.1.37). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 1.1.1.37 |
| Sequence | >tr|Q4Q3J2|Q4Q3J2_LEIMA Malate dehydrogenase, putative (EC 1.1.1.37) - Leishmania major. RSSATRLFRVAVLGAAGGIGQPLSLLLKCSPLVTSLSLYDIRGGPGVAADLSHIPSPAEV IGFSSGELEKAVKGADLVLVVAGIPRKPGMTRDDLLHTNASIVRDLAIAVGTHAPKAIVG IITNPVNSTVPVAAEALKKVGVYDPARLFGVTTLDVVRARTFVAEALGASPYDVDVPVIG GHSGETIVPLLSGFPSLSEEQVRQLTHRIQFGGDEVVKAKDGAGSATLSMAYAASEWSIS MLKALRGDRGIVEYALVESDMQQPHSRFFGCAVELGTHGVERVLPMPKLNAYEQQLLDAC VPALSAEFRKGVDLAVKLHLSQDN |
| DNA Sequence | >LmjF34.0160 |||malate dehydrogenase, putative|Leishmania major|chr 34|||Manual ATGCGTTCCT CTGCGACTCG GCTGTTCCGC GTTGCCGTCC TGGGAGCGGC TGGCGGCATCGGCCAGCCCT TGTCGCTCCT CCTAAAGTGC AGCCCTCTCG TGACGAGTCT TTCGCTCTATGACATTCGCG GCGGACCAGG CGTGGCTGCG GACCTCTCCC ACATTCCATC ACCAGCAGAGGTGATCGGGT TTTCCTCAGG TGAGCTGGAG AAAGCCGTGA AGGGAGCGGA CCTCGTGCTGGTGGTGGCTG GCATCCCCCG CAAACCGGGG ATGACGCGCG ACGACCTCTT GCACACCAACGCCAGCATTG TCCGAGATCT CGCCATCGCC GTTGGCACGC ACGCACCGAA GGCAATTGTTGGCATTATCA CGAACCCCGT CAACAGCACT GTGCCAGTGG CTGCTGAGGC GCTGAAGAAGGTCGGCGTGT ACGATCCTGC GCGCCTCTTT GGTGTCACCA CACTCGACGT TGTCCGTGCCCGCACCTTCG TCGCGGAGGC GCTCGGTGCC AGCCCGTACG ACGTTGACGT CCCTGTTATTGGCGGCCACA GCGGTGAGAC GATTGTGCCG TTGCTCTCGG GCTTCCCGTC GCTGTCGGAGGAGCAGGTGC GGCAGCTGAC GCACCGCATC CAGTTTGGTG GTGATGAGGT GGTGAAGGCCAAGGATGGTG CGGGCTCGGC GACGCTGTCC ATGGCGTACG CGGCCTCGGA ATGGTCAATCTCGATGCTCA AGGCTCTTCG TGGTGACAGG GGCATTGTCG AGTACGCTCT TGTGGAAAGTGACATGCAGC AGCCACATTC TCGCTTCTTT GGATGTGCAG TGGAACTTGG CACACACGGCGTAGAGCGTG TGCTGCCGAT GCCGAAGCTG AATGCCTACG AGCAGCAGTT GCTGGACGCGTGCGTTCCTG CACTCAGCGC CGAGTTCCGC AAGGGGGTCG ACCTTGCTGT GAAGTTGCACCTCTCACAGG ATAATTGA |
| Malate dehydrogenase, putative Q4Q3J2] | |
|---|---|
| Metabolite Information | malate metabolism; tricarboxylic acid cycle intermediate metabolism |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K00026 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | mitochondrial malate dehydrogenase precursor [Homo sapiens] | 52 | 4e-85 | 311 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| mdh malate dehydrogenase | Haemophilus influenzae | 54% | 7e-79 | 288 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00016 | Cell attachment sequence | 247-249; | PS00016 | |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 100-103; 128-131; | PS00722 | |
| PDOC00066 | Malate dehydrogenase active site signature | 152-164; | PS00068 | |
| Acylation | N-myristoylation site | 15-20; 21-26; 181-186; 223-228; 312-317; | PS00008 | |
| Glycosylation | N-glycosylation site | 100-103; 128-131; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 38-41; 57-60; 65-68; 92-95; 153-156; 171-174; 197-200; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 207-209; | PS00005 | |
| Malate dehydrogenase, putative [Q4Q3J2] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1smkH | |
| Percent Identity | 54% | |
| Target Region | 1-325 | |
| Template Region | 44-313 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 156 | Sidechain |
| HIS | 183 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 5949-29-1 | CITRIC ACID | 192.124 | C6 H8 O7 | O=C(O)CC(O)(C(=O)O)CC(=O)O | 1smk |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/137490/Q4Q3J2.pdb 2.0 325 = residues | | = | +| Ramachandran plot: 91.9% core 7.0% allow 1.1% gener 0.0% = disall | | = | *| All Ramachandrans: 10 labelled residues (out of 323) = | +| Chi1-chi2 plots: 2 labelled residues (out of 159) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 15.8 Bad contacts: = 6 | *| Bond len/angle: 8.1 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.04 Covalent: -0.18 Overall: = -0.04 | | = | | M/c bond lengths: 99.2% within limits 0.8% highlighted = | *| M/c bond angles: 94.4% within limits 5.6% highlighted 3 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |