| DNA-directed RNA polymerase subunit, putative [Q4Q3B6] | |
|---|---|
| Systematic Name | LmjF.34.0890 [Leishmania major] |
| Gene Name | LMJF_34_0890 |
| Molecular Weight | 17521 Da |
| Protein Sequence Size | 159 |
| Function | |
| Charge | -17 |
| Isoelectric Point | 4.104 pH |
| Description | DNA-directed RNA polymerase subunit, putative (EC 2.7.7.6). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 2.7.7.6 |
| Sequence | >tr|Q4Q3B6|Q4Q3B6_LEIMA DNA-directed RNA polymerase subunit, putative (EC 2.7.7.6) - Leishmania major. SDHDDEDDLLSLNLGDDRDEGDSGSDIRHSGADEEDEEGGEQLPEDVAANVILSGNAQAA KGARARGINERVTSAVMTKYERARVLGTRALQISMNAPVAVALEGETDPLTIAVKELRER RTPLIIRRVLPDNTYEDWSVSELLVDFDRPADERYTNI |
| DNA Sequence | >LmjF34.0890 |||DNA-directed RNA polymerase subunit, putative|Leishmania major|chr 34|||Manual ATGTCGGACC ACGACGACGA AGACGATCTC CTCAGCCTCA ATCTAGGAGA CGACCGTGATGAGGGCGACA GCGGCAGCGA CATTCGACAC AGCGGCGCTG ATGAGGAGGA CGAGGAGGGAGGCGAGCAGC TGCCCGAGGA CGTTGCCGCC AACGTCATCT TGTCCGGCAA TGCGCAGGCCGCAAAGGGTG CACGGGCCCG CGGAATCAAC GAGCGCGTGA CGAGTGCTGT CATGACCAAGTATGAGCGGG CACGCGTGCT GGGTACGCGC GCGCTGCAGA TAAGCATGAA CGCCCCTGTCGCTGTGGCGC TCGAGGGCGA AACCGATCCG CTCACGATTG CCGTAAAGGA GCTGCGTGAGCGCCGCACGC CGCTTATCAT TCGTCGCGTG CTGCCGGACA ACACGTACGA GGACTGGAGCGTCAGCGAGC TGCTCGTCGA CTTTGATCGC CCGGCGGATG AGCGCTACAC GAACATCTAA |
| DNA-directed RNA polymerase subunit, putative Q4Q3B6] | |
|---|---|
| Metabolite Information | transcription, DNA-dependent |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K03014 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | DNA directed RNA polymerase II polypeptide F [Homo sapiens] | 65 | 5e-26 | 114 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YPR187w DNA-directed RNA polymerase I, II, III 18 KD subunit | Saccharomyces cerevisiae | 71% | 5e-28 | 117 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00029 | Leucine zipper pattern | 104-125; | PS00029 | |
| PDOC00855 | RNA polymerases K / 14 to 18 Kd subunits signature | 79-93; | PS01111 | |
| Acylation | N-myristoylation site | 56-61; | PS00008 | |
| Phosphorylation | Casein kinase II phosphorylation site | 2-5; 24-27; 31-34; 79-82; 135-138; 140-143; | PS00006 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 129-136; 150-156; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 129-143; | PS00003 | |
| DNA-directed RNA polymerase subunit, putative [Q4Q3B6] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2yu9F | |
| Percent Identity | 71% | |
| Target Region | 64-147 | |
| Template Region | 71-84 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
|
|
|
| Modeled Protein | Template Structure |
|
|
+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/2635110/Q4Q3B6.pdb 2.0 84 = residues | | = | +| Ramachandran plot: 92.0% core 6.7% allow 1.3% gener 0.0% = disall | | = | *| All Ramachandrans: 4 labelled residues (out of 82) = | +| Chi1-chi2 plots: 1 labelled residues (out of 48) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 2.6 Bad contacts: = 1 | *| Bond len/angle: 6.3 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: -0.03 Covalent: -0.25 Overall: = -0.10 | | = | | M/c bond lengths: 99.3% within limits 0.7% highlighted = | | M/c bond angles: 92.7% within limits 7.3% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |