| ATP-dependent DNA helicase, putative [Q4Q2T4] | |
|---|---|
| Systematic Name | LmjF.34.2610 [Leishmania major] |
| Gene Name | LMJF_34_2610 |
| Molecular Weight | 53638 Da |
| Protein Sequence Size | 483 |
| Function | |
| Charge | -10.5 |
| Isoelectric Point | 5.2 pH |
| Description | ATP-dependent DNA helicase, putative (Ruvb dna helicase-like protein, putative) (EC 3.6.1.-). |
| Subcellular Location | nucleus[Predict] |
| E. C. Number | 3.6.1.- |
| Sequence | >tr|Q4Q2T4|Q4Q2T4_LEIMA ATP-dependent DNA helicase, putative (Ruvb dna helicase-like protein, putative) (EC 3.6.1.-) - Leishmania major. AEGPIRTAEARDLTRVERVGAHSHIRGLGLDDTLEARVSSQGMVGQMEARRAAGVVVQMV KKGKIAGRCVLLAGGPGSGKTAIAMAMAQALGPETPFTMIAGSEIFSLEMSKTEALTQAF RRSIGVHIKEETEMIEGEVVEVTIDRPSTNPAEAHQRTGQLVLKTSDMESTFDLGQKMIE SLQKEKVQVGDVITIDKATGRISKLGRSFVHSKDFDAMSANTRFVQTPEGELSKRKEVVH TVTLHEVDVINSRQQGFLALFAGDTGEIKPEVREQIDQRVAEWREEGKGEIVPGVLFIDE VHMLDIECFSWLNRALESPLAPVVIVASNRGISRIRGTQYKAPHGIPIDLLDRMMIITTN PYSQEELGKIINIRCEEEDVELTEDAFVLLTTLGQKTSLRYVLQLITTANMVAQKRKSST VSVDDIKKVYLLFIDLRRSVELLQEHEKDFLFGEEDTHVENSTRVRVRDGQEDCGNEEET VR |
| DNA Sequence | >LmjF34.2610 |||ATP-dependent DNA helicase, putative|Leishmania major|chr 34|||Manual ATGGCGGAGG GTCCCATTCG TACTGCGGAG GCTCGCGACC TCACCCGAGT GGAGCGCGTCGGCGCACATT CCCACATTCG CGGCCTCGGC CTCGATGACA CGCTCGAGGC GCGCGTTTCGAGTCAGGGCA TGGTGGGTCA AATGGAGGCT CGGCGTGCGG CGGGCGTGGT GGTGCAGATGGTGAAGAAGG GAAAGATCGC CGGCCGGTGT GTGTTGTTGG CTGGCGGGCC GGGCTCTGGTAAGACGGCCA TTGCGATGGC CATGGCGCAG GCGCTTGGAC CCGAGACACC CTTCACCATGATTGCTGGTA GCGAGATCTT TTCCCTTGAG ATGTCCAAGA CGGAGGCGCT GACGCAGGCGTTTCGTCGTA GCATTGGCGT GCACATCAAG GAGGAGACGG AGATGATCGA GGGCGAGGTGGTGGAGGTGA CCATTGATCG CCCGTCTACG AATCCCGCCG AGGCCCATCA GCGCACCGGGCAACTGGTGC TCAAGACTTC CGACATGGAG TCGACCTTCG ACCTGGGGCA GAAGATGATCGAGAGCTTGC AGAAGGAAAA GGTCCAAGTA GGCGACGTCA TCACGATTGA CAAGGCCACCGGCCGCATCA GCAAACTGGG CCGCAGCTTT GTGCATAGCA AGGACTTTGA CGCCATGTCCGCCAACACGC GCTTCGTGCA GACACCGGAG GGCGAGCTGT CGAAGCGGAA GGAGGTGGTGCACACCGTGA CGCTGCATGA GGTGGACGTG ATCAACTCTC GCCAGCAGGG CTTCCTCGCTCTCTTTGCCG GTGACACCGG TGAGATCAAG CCCGAGGTGC GCGAGCAGAT TGATCAGCGCGTCGCTGAAT GGCGGGAGGA GGGTAAGGGC GAGATCGTTC CCGGTGTACT CTTTATTGACGAGGTCCACA TGCTCGATAT CGAGTGCTTC TCATGGCTGA ACCGCGCGCT GGAAAGCCCATTAGCGCCGG TGGTGATCGT CGCGTCGAAT CGTGGTATTT CGCGTATCCG CGGTACACAGTACAAGGCCC CACATGGCAT CCCGATCGAC CTGCTGGATC GAATGATGAT TATCACCACAAACCCGTACT CGCAGGAGGA GCTCGGCAAG ATTATTAACA TTCGGTGCGA GGAGGAGGATGTGGAGCTGA CAGAGGACGC CTTTGTCCTC CTGACGACAC TTGGCCAGAA GACGTCGCTGCGCTACGTGC TGCAGCTCAT TACGACGGCG AACATGGTGG CGCAGAAGCG GAAGTCGTCGACCGTGTCGG TGGACGACAT CAAGAAGGTG TATCTTCTCT TCATCGACCT CCGCCGCAGCGTGGAGCTGC TGCAGGAGCA CGAGAAGGAC TTTCTGTTTG GCGAGGAGGA CACGCACGTCGAGAACTCGA CTCGCGTGCG CGTACGGGAC GGTCAGGAAG ATTGCGGCAA CGAGGAGGAGACGGTGCGGT AA |
| ATP-dependent DNA helicase, putative Q4Q2T4] | |
|---|---|
| Metabolite Information | |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K01529 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | RuvB-like 2 [Homo sapiens] | 67 | 1e-176 | 615 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YPL235w RUVB-like protein | Saccharomyces cerevisiae | 60% | 1e-155 | 543 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00017 | ATP/GTP-binding site motif A (P-loop) | 75-82; | PS00017 | |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 462-465; | PS00722 | |
| Acylation | N-myristoylation site | 30-35; 46-51; 75-80; 78-83; 395-400; 471-476; | PS00008 | |
| Glycosylation | N-glycosylation site | 462-465; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 416-419; 417-420; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 15-18; 112-115; 167-170; 171-174; 244-247; 364-367; 423-426; 458-461; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 79-81; 200-202; 234-236; 329-331; 399-401; 463-465; 481-483; | PS00005 | |
| ATP-dependent DNA helicase, putative [Q4Q2T4] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2c9oC | |
| Percent Identity | 41% | |
| Target Region | 12-447 | |
| Template Region | 7-311 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 58-64-0 | ADENOSINE-5'-DIPHOSPHATE | 427.201 | C10 H15 N5 O10 P2 | O=P(O)(O)OP(=O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O | 2c9o |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/5310177/Q4Q2T4.pdb 2.0 435 = residues | | = | +| Ramachandran plot: 92.5% core 7.0% allow 0.5% gener 0.0% = disall | | = | +| All Ramachandrans: 5 labelled residues (out of 433) = | +| Chi1-chi2 plots: 2 labelled residues (out of 258) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 3.0 Bad contacts: = 1 | *| Bond len/angle: 5.7 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.15 Covalent: -0.12 Overall: = 0.05 | | = | | M/c bond lengths: 99.5% within limits 0.5% highlighted = | | M/c bond angles: 95.1% within limits 4.9% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |