| Elongation factor 2 [Q4Q259] | |
|---|---|
| Systematic Name | LmjF.36.0180 [Leishmania major] |
| Gene Name | EF2-2 |
| Molecular Weight | 94133 Da |
| Protein Sequence Size | 845 |
| Function | |
| Charge | -5.5 |
| Isoelectric Point | 5.9481 pH |
| Description | Elongation factor 2. |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4Q259|Q4Q259_LEIMA Elongation factor 2 - Leishmania major VNFTVDQVRELMDYPDQIRNMSVIAHVDHGKSTLSDSLVGAAGIIKMEEAGDKRIMDTRA DEIARGITIKSTAISMHYHVPKEMIGDLDDDKRDFLINLIDSPGHVDFSSEVTAALRVTD GALVVVDCVEGVCVQTETVLRQALTERIRPVVFINKVDRAILELQLDPEEAYQGFVKTLQ NVNVVVATYNDPSMGDVQVSPEKGTVAIGSGLQAWAFSLTRFANMYAAKFGVDELKMRER LWGDNFFDAKNKKWIKQETNADGERVRRAFCQFCLDPIYQIFDAVMNEKKDKVDKMLKSL HVTLTAEEREQVPKKLLKTVMMKFLPAAETLLQMIVAHLPSPKKAQAYRAEMLYSGEASP EDKYFMGIKNCDPAAPLMLYISKMVPTADRGRFFAFGRIFSGKVRSGQKVRIMGNNYVYG KKQDLYEDKPVQRSVLMMGRYQEAVEDMPCGNVVGLVGVDKYIVKSATITDDGESPHPLR DMKYSVSPVVRVAVEAKNPSDLPKLVEGLKRLAKSDPLVVCSIEESGEHIVAGAGELHLE ICLKDLQEDFMNGAPLKISEPVVSFRETVTDVSSQQCLSKSANKHNRLFCRGAPLTEELA LAMEEGTAGPEADPKVRARFLADNYEWDVQEARKIWCYGPDNRGPNVVVDVTKGVQNMAE MKDSFVAAWQWATREGVLCDENMRGVRVNVEDVTMHADAIHRGGGQIIPTARRVFYACCL TASPRLMEPMFVVDIQTVEHAMGGIYGVLTRRRGVIIGEENRPGTPIYNVRAYLPVAESF GFTADLRAGTGGQAFPQCVFDHWQEYPGDPLEPKSLANTTTLGIRTRKGLKPDIPGLDQF MDKL |
| DNA Sequence | >LmjF36.0180 |EF2-1||elongation factor 2|Leishmania major|chr 36|||Manual ATGGTGAACT TTACCGTCGA TCAGGTCCGC GAGCTGATGG ACTATCCGGA CCAGATCCGGAACATGTCCG TGATTGCTCA CGTCGACCAC GGCAAGTCGA CGCTGTCCGA CTCTCTCGTTGGTGCTGCCG GCATCATCAA GATGGAGGAG GCTGGCGATA AGCGGATCAT GGATACACGCGCGGATGAGA TCGCGCGTGG TATCACGATC AAGTCCACCG CCATCTCCAT GCACTACCACGTGCCGAAGG AGATGATCGG CGATCTGGAT GACGACAAGC GCGACTTCCT GATCAACCTGATCGACTCCC CCGGACACGT CGACTTCAGC TCCGAGGTGA CTGCCGCTCT TCGTGTGACGGACGGCGCGC TGGTCGTGGT GGACTGCGTG GAGGGCGTGT GCGTGCAGAC GGAGACGGTGCTGCGCCAGG CGCTGACGGA GCGCATCCGC CCTGTTGTGT TCATCAACAA GGTGGACCGCGCCATCCTTG AGCTCCAACT GGACCCCGAG GAGGCGTACC AGGGCTTCGT GAAGACGCTGCAGAACGTGA ACGTGGTGGT TGCCACGTAC AATGACCCCA GCATGGGCGA CGTGCAGGTGTCCCCCGAGA AGGGCACTGT GGCGATCGGC TCTGGCCTGC AGGCGTGGGC GTTCTCGCTGACCCGCTTTG CGAACATGTA TGCGGCGAAG TTCGGCGTGG ACGAGCTGAA GATGCGCGAGCGCCTGTGGG GCGACAACTT CTTTGACGCG AAGAACAAGA AGTGGATCAA GCAGGAGACGAACGCCGATG GCGAGCGCGT GCGCCGCGCG TTCTGCCAGT TCTGCCTAGA CCCCATCTACCAGATCTTCG ACGCTGTGAT GAACGAGAAG AAGGACAAGG TGGACAAGAT GCTCAAGTCGCTGCACGTGA CGCTGACGGC TGAGGAGCGC GAGCAGGTGC CGAAGAAGCT TCTGAAGACGGTGATGATGA AGTTCCTGCC GGCTGCTGAG ACGCTGCTAC AGATGATCGT GGCGCACCTGCCGTCGCCCA AGAAGGCGCA GGCGTACCGT GCGGAGATGC TGTACTCTGG CGAGGCGTCGCCGGAGGACA AGTACTTCAT GGGTATCAAG AACTGCGACC CCGCTGCGCC GCTCATGCTGTACATCAGCA AGATGGTGCC GACGGCCGAC CGCGGCCGCT TCTTCGCCTT TGGCCGCATCTTCTCCGGTA AGGTGCGCAG CGGCCAGAAG GTGCGCATCA TGGGTAACAA CTACGTCTACGGCAAGAAGC AGGACCTGTA CGAGGACAAG CCTGTGCAGC GCTCCGTGCT GATGATGGGCCGCTACCAGG AGGCCGTGGA GGACATGCCG TGCGGTAACG TGGTGGGCCT TGTGGGCGTGGACAAGTACA TCGTGAAGTC CGCGACGATC ACGGACGATG GCGAGAGCCC GCACCCGCTGCGCGACATGA AGTACTCTGT GTCGCCCGTC GTGCGTGTGG CCGTGGAGGC GAAGAACCCGTCCGACCTGC CGAAGCTTGT GGAGGGCCTG AAGCGCCTTG CCAAGTCCGA CCCGCTGGTGGTGTGCAGCA TTGAGGAGTC TGGCGAGCAC ATTGTTGCCG GCGCTGGCGA GCTTCACCTTGAGATTTGCC TGAAGGATCT CCAGGAGGAC TTCATGAACG GCGCGCCGCT GAAGATCTCCGAGCCGGTGG TGTCGTTCCG CGAGACGGTG ACGGATGTGT CGTCGCAGCA GTGCCTGTCGAAGTCTGCGA ACAAGCACAA CCGTCTCTTC TGCCGCGGTG CGCCGCTGAC AGAGGAGCTGGCGCTGGCGA TGGAGGAGGG CACCGCTGGT CCCGAGGCGG ATCCGAAGGT GCGCGCGCGCTTCCTTGCCG ACAACTACGA GTGGGACGTG CAGGAGGCCC GCAAGATCTG GTGCTACGGCCCGGACAACC GCGGCCCGAA CGTGGTCGTG GATGTGACGA AGGGTGTCCA GAACATGGCTGAGATGAAGG ACTCCTTTGT TGCGGCGTGG CAGTGGGCGA CCCGCGAGGG TGTGCTGTGCGACGAGAACA TGCGCGGCGT GCGCGTGAAC GTGGAGGATG TGACGATGCA CGCGGATGCCATTCACCGTG GTGGTGGCCA GATCATCCCG ACGGCGCGCC GCGTGTTCTA CGCGTGCTGCCTGACGGCGT CGCCGCGCCT GATGGAGCCG ATGTTCGTGG TGGACATCCA GACCGTGGAGCACGCCATGG GCGGCATCTA CGGTGTGCTG ACCCGCCGCC GTGGCGTGAT CATTGGCGAGGAGAACCGCC CCGGCACGCC CATCTACAAC GTGCGCGCGT ACCTGCCGGT TGCGGAGTCGTTCGGCTTCA CTGCCGACCT GCGCGCTGGA ACTGGCGGCC AGGCCTTCCC GCAGTGTGTGTTCGACCACT GGCAGGAGTA CCCCGGTGAC CCGCTGGAGC CGAAGTCGCT GGCCAACACGACGACGCTTG GCATCCGCAC GCGCAAGGGC CTGAAGCCGG ATATCCCCGG CCTGGACCAGTTCATGGATA AGTTGTAA |
| Elongation factor 2 Q4Q259] | |
|---|---|
| Metabolite Information | protein biosynthesis |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K03234 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | eukaryotic translation elongation factor 2 [Homo sapiens] | 60 | 0 | 1025 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YKL173w U5 snRNP-specific protein | Saccharomyces cerevisiae | 26% | 1e-72 | 269 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00017 | ATP/GTP-binding site motif A (P-loop) | 26-33; | PS00017 | |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 3-6; 21-24; 819-822; | PS00722 | |
| PDOC00015 | The rule PS00015 has been deleted from the PROSITE database and replaced by the following motif: PS50079 (Bipartite nuclear localization signal profile | 253-269; | PS00015 | |
| Acylation | N-myristoylation site | 368-373; 452-457; 593-598; 686-691; 744-749; 755-760; | PS00008 | |
| Amidation | Amidation site | 420-423; | PS00009 | |
| Glycosylation | N-glycosylation site | 3-6; 21-24; 819-822; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 34-37; 59-62; 189-192; 194-197; 260-263; 306-309; 360-363; 469-472; 523-526; 565-568; 569-572; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 69-71; 146-148; 342-344; 402-404; 565-567; 711-713; 724-726; 751-753; 827-829; | PS00005 | |
| Sulfation | Tyrosine sulfation site | 166-180; 800-814 | PS00003 | |
| Elongation factor 2 [Q4Q259] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2p8zT | |
| Percent Identity | 60% | |
| Target Region | 1-845 | |
| Template Region | 3-813 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 29 | Sidechain |
| HIS | 106 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| ADENOSINE-5-DIPHOSPHORIBOSE | 559.316 | C15 H23 N5 O14 P2 | O=P(O)(OCC3OC(n1c2ncnc(N)c2nc1)C(O)C3O)OP(=O)(O)OCC4OC(O)C(O)C4O | 2p8z | |
| PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER | 522.196 | C10 H17 N6 O13 P3 | O=P(O)(O)NP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c2N=C(N)NC1=O)C(O)C3O | 2p8z | |
| 11076-17-8 | SORDARIN | 494.618 | C27 H42 O8 | O=C(O)C53C4(COC1OC(C(OC)C(O)C1O)C)CC2C(C)CCC2C3(C=O)CC4CC5C(C)C | 2p8z |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/2061268/Q4Q259.pdb 2.0 845 = residues | | = | *| Ramachandran plot: 90.9% core 8.0% allow 0.8% gener 0.3% = disall | | = | +| All Ramachandrans: 22 labelled residues (out of 843) = | +| Chi1-chi2 plots: 12 labelled residues (out of 496) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 9.8 Bad contacts: = 22 | *| Bond len/angle: 21.2 Morris et al class: 1 = 1 2 | *| 1 cis-peptides 1 D amino acids = | +| G-factors Dihedrals: 0.03 Covalent: -0.73 Overall: = -0.26 | | = | *| M/c bond lengths: 97.5% within limits 2.5% highlighted 27 off = graph | *| M/c bond angles: 92.4% within limits 7.6% highlighted 30 off = graph | +| Planar groups: 99.7% within limits 0.3% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |