| Short chain 3-hydroxyacyl-coa dehydrogenase, putative [Q4Q1W2] | |
|---|---|
| Systematic Name | LmjF.36.1140 [Leishmania major] |
| Gene Name | LMJF_36_1140 |
| Molecular Weight | 32973 Da |
| Protein Sequence Size | 305 |
| Function | |
| Charge | 4.5 |
| Isoelectric Point | 8.3513 pH |
| Description | Short chain 3-hydroxyacyl-coa dehydrogenase, putative (EC 1.1.1.35). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 1.1.1.35 |
| Sequence | >tr|Q4Q1W2|Q4Q1W2_LEIMA Short chain 3-hydroxyacyl-coa dehydrogenase, putative (EC 1.1.1.35) - Leishmania major LRCTASALFKSLAVWGGGTMGSGIAQITAQAAVPVTVVEVSQARLDASRKAIETSLLRIA NKQCNGDQGKMKAFVDTVLSHVTFTTDEAVAAGGAELIIEAIVENIGAKKELFGRLDKMA PKSTVFCSNTSSLSITEMASATTRKDRFAGMHFFSPVPMMKLLEVVRTEEVSPAIIEQLT AFGKKVGKIPVVAKDTEGFIVNRLLVPYQMEACRLVERGVATFQDVDTAMKFGCGYPMGP FELCDSVGIDVIKFIVDGWHAQYPNEPLFKPSPLIDERVAAGKLGKKTGEGYYKYDGKGR KIES |
| DNA Sequence | >LmjF36.1140 |||short chain 3-hydroxyacyl-coa dehydrogenase, putative|Leishmania major|chr 36|||Manual ATGCTTCGTT GCACCGCCTC TGCGCTTTTC AAGTCGCTTG CCGTGTGGGG CGGCGGCACGATGGGCAGCG GCATCGCGCA GATCACAGCT CAGGCGGCTG TCCCGGTGAC TGTGGTGGAGGTCTCGCAAG CGCGGCTGGA CGCGTCGCGC AAGGCGATCG AGACGTCGCT GCTGCGCATCGCCAACAAGC AGTGCAACGG TGATCAGGGC AAGATGAAGG CATTCGTGGA CACGGTGCTTTCTCACGTCA CGTTCACGAC GGACGAAGCC GTTGCTGCTG GCGGCGCGGA GCTGATCATCGAAGCGATTG TGGAGAACAT CGGCGCGAAA AAGGAGCTCT TTGGCCGCCT GGACAAGATGGCGCCGAAGT CGACGGTGTT CTGCTCGAAC ACGTCGTCTC TGAGCATCAC GGAGATGGCGTCCGCAACGA CACGCAAGGA CCGATTTGCC GGCATGCACT TCTTCTCGCC CGTGCCGATGATGAAGCTGC TCGAGGTGGT GCGCACAGAG GAGGTGAGTC CAGCCATCAT TGAGCAGCTTACCGCCTTCG GCAAGAAGGT AGGCAAGATA CCTGTTGTTG CGAAGGACAC GGAGGGCTTCATCGTGAACC GCCTTCTCGT GCCGTATCAA ATGGAGGCGT GTAGACTAGT AGAGCGTGGCGTCGCGACTT TCCAGGATGT GGACACTGCT ATGAAGTTCG GCTGCGGCTA CCCGATGGGACCGTTTGAAC TTTGTGACAG TGTTGGCATC GACGTTATCA AGTTCATTGT CGACGGCTGGCATGCTCAGT ACCCCAACGA GCCGCTCTTC AAGCCCTCAC CCCTAATTGA TGAACGGGTTGCCGCTGGCA AGCTGGGTAA GAAGACTGGC GAGGGCTACT ACAAGTACGA CGGCAAGGGCCGCAAAATCG AATCCTGA |
| Short chain 3-hydroxyacyl-coa dehydrogenase, putative Q4Q1W2] | |
|---|---|
| Metabolite Information | fatty acid metabolism |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K00022 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain [Homo sapiens] | 51 | 5e-78 | 288 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| fadB3 PROBABLE 3-HYDROXYBUTYRYL-CoA DEHYDROGENASE FADB3 (BETA-HYDROXYBUTYRYL-CoA DEHYDROGENASE) (BHBD) | Mycobacterium tuberculosis H37Rv | 30% | 3e-27 | 116 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 130-133; | PS00722 | |
| PDOC00015 | The rule PS00015 has been deleted from the PROSITE database and replaced by the following motif: PS50079 (Bipartite nuclear localization signal profile | 287-303; | PS00015 | |
| Acylation | N-myristoylation site | 18-23; 19-24; 22-27; | PS00008 | |
| Amidation | Amidation site | 183-186; 285-288; 299-302; | PS00009 | |
| Glycosylation | N-glycosylation site | 130-133; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 37-40; 86-89; 135-138; 144-147; 223-226; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 49-51; 143-145; 144-146; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 287-294; | PS00007 | |
| Short chain 3-hydroxyacyl-coa dehydrogenase, putative [Q4Q1W2] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 3hdhC | |
| Percent Identity | 52% | |
| Target Region | 9-290 | |
| Template Region | 14-265 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| SER | 124 | Unknown |
| HIS | 145 | Unknown |
| ASN | 195 | Unknown |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| NICOTINAMIDE-ADENINE-DINUCLEOTIDE | 663.425 | C21 H27 N7 O14 P2 | c1cc(c[n+](c1)C2C(C(C(O2)COP(=O)([O-])OP(=O)(O)OCC3C(C(C(O3)n4cnc5c4ncnc5N)O)O)O)O)C(=O)N | 3hdh | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1182200/Q4Q1W2.pdb 2.0 282 = residues | | = | +| Ramachandran plot: 91.0% core 7.8% allow 1.2% gener 0.0% = disall | | = | *| All Ramachandrans: 12 labelled residues (out of 280) = | +| Chi1-chi2 plots: 1 labelled residues (out of 148) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 6.7 Bad contacts: = 2 | *| Bond len/angle: 12.1 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: -0.02 Covalent: -0.43 Overall: = -0.16 | | = | *| M/c bond lengths: 98.6% within limits 1.4% highlighted 1 off = graph | *| M/c bond angles: 89.8% within limits 10.2% highlighted 3 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |