| Chaperonin Hsp60, mitochondrial [Q4Q1M1] | |
|---|---|
| Systematic Name | LmjF.36.2020 [Leishmania major] |
| Gene Name | LMJF_36_2020 |
| Molecular Weight | 60180 Da |
| Protein Sequence Size | 565 |
| Function | |
| Charge | -7 |
| Isoelectric Point | 5.1201 pH |
| Description | Chaperonin Hsp60, mitochondrial. |
| Subcellular Location | mitochondrial matrix; Mitochondrion[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4Q1M1|Q4Q1M1_LEIMA Chaperonin Hsp60, mitochondrial - Leishmania major LRSAVCLAGKDVRFGEEARRSMQKGVTRAVAAVATTLGPKGRNVIIEQAYGAPKITKDGV TVAKAIEFKDRFENMGAQLVRQVCNQTNDLAGDGTTTSAVLVDSIFGEGLKCIAQGTNPI DMKRGMDVAVHYVQTSLLKQSRPIKGTEDIVRVATISANGDKEIGEMIGQAMDKVGRDGV ITAQDGKTMATELEVVEGMKIERGFLSPYFVTDAKVQKAELEDLNVLVSKKKISTIQTLL PALNHVAQQGRPLLIIADDVESEALTTLIFNKLQGKLKVCCVKAPGFGDTKEGMLEDIAV FTGAKVVGDENTGVELDARSFDPSILGSAKKVTVTKDDTVMLNGGGDAAAVKERQQLLRD RIEQEAVEYNREKLQERLAKLSGGVAVIKVGGGSEVEVSEKKDRVVDAVCSTRAAVQEGI VAGGGTALLRASKELEALANDGSLTRDQRTGVTIVRNAIRLPAMKIAANAGKEGAVIVEK VLEASEESTGYDAQNDKYVNMFEAGIIDPTRVVRVAISDATSVASLMMTAEAAIVEAPKE SKAKNGKRSAAAETEDDEELFGSY |
| DNA Sequence | >LmjF36.2020 |||chaperonin Hsp60, mitochondrial precursor|Leishmania major|chr 36|||Manual ATGCTCCGCT CCGCTGTGTG CCTTGCCGGC AAGGACGTGC GCTTTGGCGA AGAGGCCCGCCGCTCCATGC AGAAGGGCGT CACTCGCGCT GTGGCTGCGG TAGCGACGAC GCTGGGGCCGAAGGGTCGCA ACGTGATCAT CGAGCAGGCC TACGGCGCGC CGAAGATCAC GAAGGATGGTGTGACCGTTG CCAAGGCGAT CGAGTTCAAG GATCGCTTCG AGAACATGGG TGCGCAGCTGGTACGCCAGG TGTGCAACCA GACGAACGAC TTGGCTGGCG ACGGCACCAC GACCTCTGCAGTGCTTGTGG ACAGCATCTT TGGCGAGGGC CTCAAGTGCA TTGCCCAGGG CACGAACCCGATCGACATGA AGCGTGGCAT GGATGTCGCG GTCCACTACG TACAGACGAG CCTGCTGAAGCAGTCCCGCC CGATCAAGGG AACTGAGGAT ATCGTTCGCG TTGCCACCAT CTCGGCCAACGGCGATAAGG AGATCGGCGA GATGATCGGT CAGGCGATGG ACAAGGTCGG TCGCGACGGCGTCATTACGG CGCAGGACGG CAAGACGATG GCCACTGAGC TGGAGGTTGT GGAGGGCATGAAGATCGAGC GTGGGTTTCT CAGCCCGTAC TTCGTGACGG ATGCGAAGGT GCAGAAGGCAGAGCTCGAGG ACTTGAATGT GCTTGTATCC AAGAAGAAAA TTAGCACCAT CCAGACGTTGCTTCCCGCCC TCAACCACGT CGCTCAGCAA GGCCGCCCGC TTCTGATCAT CGCCGACGATGTGGAGAGCG AGGCGCTGAC AACGCTGATC TTCAACAAGT TGCAGGGCAA GCTGAAGGTGTGCTGCGTCA AGGCGCCGGG CTTCGGCGAC ACTAAGGAGG GCATGCTCGA GGATATCGCTGTTTTCACTG GCGCTAAAGT GGTGGGAGAC GAGAACACCG GCGTGGAGCT GGATGCCAGGAGCTTCGACC CCTCCATCCT CGGCTCTGCA AAGAAGGTGA CAGTGACGAA GGACGATACAGTGATGCTGA ACGGCGGCGG CGATGCGGCG GCGGTGAAGG AGCGCCAGCA GTTGCTGCGCGATCGCATCG AGCAGGAGGC TGTGGAGTAC AATCGGGAGA AGCTGCAGGA GCGTCTGGCGAAGCTGAGCG GCGGCGTGGC CGTCATCAAG GTGGGTGGCG GCTCGGAAGT GGAGGTGAGCGAGAAGAAGG ACCGCGTCGT GGACGCTGTA TGCTCCACTC GCGCTGCGGT GCAGGAGGGCATCGTGGCGG GCGGTGGCAC GGCTCTGCTG CGCGCCAGCA AGGAGCTGGA GGCTCTCGCCAACGACGGTT CCCTCACCCG CGATCAGCGC ACGGGTGTGA CCATCGTGCG TAATGCCATCCGTCTGCCGG CTATGAAGAT TGCCGCGAAC GCTGGCAAGG AGGGTGCGGT TATTGTGGAGAAGGTGCTGG AGGCTTCCGA GGAGAGCACC GGCTACGATG CGCAGAACGA CAAGTACGTGAACATGTTCG AGGCTGGCAT CATCGACCCC ACCCGCGTCG TGCGTGTGGC CATCAGCGATGCGACGTCTG TGGCGAGTCT GATGATGACG GCCGAGGCCG CCATTGTTGA GGCTCCAAAGGAGTCCAAGG CCAAGAATGG CAAGAGATCC GCTGCCGCTG AGACGGAGGA TGACGAGGAGCTGTTCGGCA GCTACTAG |
| Chaperonin Hsp60, mitochondrial Q4Q1M1] | |
|---|---|
| Metabolite Information | cellular protein metabolism; protein complex assembly; protein folding; protein import into mitochondrial matrix |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K04077 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | chaperonin [Homo sapiens] | 52 | 1e-155 | 546 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YLR259c heat shock protein - chaperone, mitochondrial | Saccharomyces cerevisiae | 51% | 1e-149 | 523 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00268 | Chaperonins cpn60 signature | 415-426; | PS00296 | |
| Acylation | N-myristoylation site | 26-31; 60-65; 95-100; 126-131; 180-185; 345-350; 346-351; 420-425; 424-429; | PS00008 | |
| Amidation | Amidation site | 546-549; | PS00009 | |
| Glycosylation | N-glycosylation site | 86-89; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 232-235; 331-334; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 183-186; 192-195; 313-316; 336-339; 395-398; 490-493; 555-558; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 230-232; 329-331; 400-402; 412-414; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 362-370; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 485-499; | PS00003 | |
| Chaperonin Hsp60, mitochondrial [Q4Q1M1] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1iokG | |
| Percent Identity | 51% | |
| Target Region | 9-539 | |
| Template Region | 2-482 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 51 | Sidechain |
| THR | 88 | Sidechain |
| THR | 89 | Sidechain |
| ASP | 400 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/2078424/Q4Q1M1.pdb 2.0 531 = residues | | = | *| Ramachandran plot: 91.0% core 8.5% allow 0.2% gener 0.2% = disall | | = | *| All Ramachandrans: 19 labelled residues (out of 529) = | +| Chi1-chi2 plots: 6 labelled residues (out of 285) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 10.0 Bad contacts: = 9 | *| Bond len/angle: 13.2 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.04 Covalent: -0.23 Overall: = -0.06 | | = | *| M/c bond lengths: 99.0% within limits 1.0% highlighted 2 off = graph | *| M/c bond angles: 95.0% within limits 5.0% highlighted 4 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |