| Cysteine synthase, putative [Q4Q159] | |
|---|---|
| Systematic Name | LmjF.36.3590 [Leishmania major] |
| Gene Name | LMJF_36_3590 |
| Molecular Weight | 35450 Da |
| Protein Sequence Size | 333 |
| Function | |
| Charge | 1.5 |
| Isoelectric Point | 6.9 pH |
| Description | Cysteine synthase, putative (EC 2.5.1.47). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 2.5.1.47 |
| Sequence | >tr|Q4Q159|Q4Q159_LEIMA Cysteine synthase, putative (EC 2.5.1.47) - Leishmania major AAPFDKSRNVAQSIDQLIGQTPALYLNKLNNTKAKVVLKMECENPMASVKDRLGFAIYDK AEKEGKLIPGKSVVVESSSGNTGVSLAHLGAIRGYKVIITMPESMSLERRCLLRIFGAEV ILTPAALGMKGAVTMAKKIVTANPNAVLADQFATKYNALIHEETTGPEIWEQTNHNVDCF IAGVGTGGTLTGVARALKKMGSHARIVAVEPMESPVLSGGKPGAHKIQGIGPGFVPDVLD RSLIDEVFCVAGDDAIETALKLTRSDGVFCGFSGGANVYAALKIAERPEMEGKTIVTIIP SFGERYLSTALYRSVRDEVSSLPVVDASELQD |
| DNA Sequence | >LmjF36.3590 |||cysteine synthase, putative|Leishmania major|chr 36|||Manual ATGGCAGCAC CGTTCGACAA GTCGAGAAAT GTGGCGCAGT CCATCGATCA GCTGATCGGCCAGACGCCGG CGTTGTACCT AAACAAGCTG AACAATACCA AGGCAAAGGT TGTGCTCAAGATGGAGTGCG AGAACCCGAT GGCGTCTGTA AAGGATCGCC TCGGCTTCGC CATCTACGATAAAGCGGAGA AGGAGGGCAA GCTGATTCCT GGCAAGTCTG TAGTGGTCGA GTCGTCCAGTGGAAACACGG GCGTGTCGCT AGCGCACTTG GGTGCAATTC GTGGTTACAA GGTCATCATTACGATGCCCG AATCCATGTC TCTCGAGCGC CGCTGCCTGC TGCGTATCTT CGGCGCCGAGGTAATCCTCA CCCCTGCCGC TCTCGGCATG AAGGGTGCCG TGACCATGGC CAAGAAAATCGTGACCGCTA ACCCCAACGC CGTCTTGGCG GATCAGTTCG CAACCAAGTA CAACGCCCTCATACACGAGG AGACCACGGG GCCTGAGATT TGGGAGCAGA CGAACCACAA TGTCGACTGCTTCATAGCCG GCGTTGGAAC GGGTGGCACG CTGACAGGTG TGGCGCGGGC GCTCAAGAAGATGGGCAGTC ATGCCCGCAT CGTTGCCGTG GAGCCGATGG AGTCACCCGT GCTGTCGGGTGGAAAACCAG GCGCGCACAA AATTCAGGGC ATCGGCCCAG GATTTGTGCC TGACGTGCTCGACCGCAGCC TCATCGACGA GGTGTTTTGC GTAGCAGGTG ATGATGCCAT TGAGACGGCACTGAAGCTCA CGCGCAGCGA CGGGGTCTTC TGCGGCTTCT CTGGCGGAGC CAACGTTTACGCGGCGCTGA AGATTGCGGA GCGTCCGGAG ATGGAGGGCA AGACAATTGT GACGATCATTCCGTCCTTCG GTGAGCGGTA CCTCTCCACC GCGTTGTACA GGAGCGTCCG GGATGAGGTGTCGTCTCTGC CAGTAGTCGA TGCCTCGGAG CTGCAGGACT GA |
| Cysteine synthase, putative Q4Q159] | |
|---|---|
| Metabolite Information | cysteine biosynthesis from serine; metabolism |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K01738 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | cystathionine-beta-synthase [Homo sapiens] | 32 | 1e-40 | 164 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| pykA pyruvate kinase | Staphylococcus aureus | 31% | 1.3 | 28.1 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00017 | ATP/GTP-binding site motif A (P-loop) | 66-73; | PS00017 | |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 31-34; | PS00722 | |
| Acylation | N-myristoylation site | 20-25; 84-89; 91-96; 129-134; 184-189; 186-191; 188-193; 189-194; 193-198; 220-225; 230-235; 268-273; | PS00008 | |
| Glycosylation | N-glycosylation site | 31-34; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 49-52; 101-104; 166-169; 243-246; 264-267; 302-305; 315-318; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 49-51; 315-317; | PS00005 | |
| Cysteine synthase, putative [Q4Q159] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2isqA | |
| Percent Identity | 47% | |
| Target Region | 9-334 | |
| Template Region | 3-320 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| LYS | 43 | Sidechain |
| SER | 294 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 54-47-7 | PYRIDOXAL-5'-PHOSPHATE | 247.142 | C8 H10 N O6 P | O=P(O)(O)OCc1cnc(c(O)c1C=O)C | 2isq |
| 14808-79-8 | SULFATE ION | 96.063 | O4 S   | [O-]S([O-])(=O)=O | 2isq |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
|
|
|
| Modeled Protein | Template Structure |
|
|
+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/491608/Q4Q159.pdb 2.0 325 = residues | | = | | Ramachandran plot: 92.1% core 7.9% allow 0.0% gener 0.0% = disall | | = | +| All Ramachandrans: 6 labelled residues (out of 323) = | +| Chi1-chi2 plots: 3 labelled residues (out of 171) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 11.6 Bad contacts: = 3 | *| Bond len/angle: 7.3 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.14 Covalent: -0.12 Overall: = 0.04 | | = | | M/c bond lengths: 99.2% within limits 0.8% highlighted = | | M/c bond angles: 95.2% within limits 4.8% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |