| Hs1vu complex proteolytic subunit-like [Q4Q116] | |
|---|---|
| Systematic Name | LmjF.36.3990 [Leishmania major] |
| Gene Name | LMJF_36_3990 |
| Molecular Weight | 23350 Da |
| Protein Sequence Size | 214 |
| Function | |
| Charge | -1.5 |
| Isoelectric Point | 5.4999 pH |
| Description | Hs1vu complex proteolytic subunit-like (Hs1vu complex proteolytic subunit-like, threonine peptidase, clan t(1), family t1b) (EC 3.4.25.-). |
| Subcellular Location | Mitochondrion; proteasome core complex (sensu Eukaryota)[Predict] |
| E. C. Number | 3.4.25.- |
| Sequence | >tr|Q4Q116|Q4Q116_LEIMA Hs1vu complex proteolytic subunit-like (Hs1vu complex proteolytic subunit-like, threonine peptidase, clan t(1), family t1b) (EC 3.4.25.-) - Leishmania major. FRRLATRSTSLVTGAAVQARHTTILSVRKGSKVILIGDRQVTLGERIVAKSSACKLRKLN DNVVIGFAGSTADAFALMEKLENKLNDFPEQLSRAAVELAKDWRTDRALRRLEASLIVCS KEETLEIDGQGNVITPEADGIIAIGSGGTYAKAAARALIDVDGYDAERIARKAMRIATDI DVFSNSNWDVEILTRDEEAVKKDEAENEAQGKE |
| DNA Sequence | >LmjF36.3990 |||hs1vu complex proteolytic subunit-like|Leishmania major|chr 36|||Manual ATGTTCCGTC GTCTTGCCAC CCGCTCCACG TCCCTCGTGA CGGGGGCCGC CGTGCAGGCGCGTCACACCA CCATTCTTTC CGTGCGAAAA GGCAGCAAAG TAATCCTTAT TGGGGACCGTCAGGTGACTT TGGGCGAGCG TATCGTGGCG AAGAGTAGCG CCTGCAAGCT GCGCAAGCTCAACGACAACG TTGTGATTGG CTTCGCCGGC AGCACGGCGG ATGCTTTTGC GTTGATGGAAAAGCTGGAAA ACAAGCTGAA CGACTTTCCG GAGCAACTGT CTCGCGCTGC GGTGGAGCTGGCAAAGGACT GGCGCACCGA CCGCGCCCTC CGCCGCCTCG AGGCATCCCT CATTGTGTGCAGCAAGGAAG AGACGTTGGA GATTGACGGG CAGGGCAACG TAATCACCCC GGAGGCTGACGGCATCATCG CCATTGGCTC GGGTGGCACG TATGCCAAGG CGGCGGCTCG TGCGCTTATTGACGTTGACG GCTATGATGC GGAGCGCATC GCGCGCAAGG CGATGAGGAT TGCAACCGATATCGATGTCT TCAGCAACAG CAACTGGGAT GTGGAGATCT TGACGCGTGA TGAAGAAGCGGTAAAGAAGG ACGAGGCGGA GAATGAGGCG CAGGGCAAGG AATAG |
| Hs1vu complex proteolytic subunit-like Q4Q116] | |
|---|---|
| Metabolite Information | ubiquitin-dependent protein catabolism |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K01419 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | SET binding factor 2 [Homo sapiens] | 44 | 0.047 | 35 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| prcB proteasome (beta subunit) PrcB | Mycobacterium tuberculosis H37Rv | 28% | 0.00002 | 43.5 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| Acylation | N-myristoylation site | 146-151; 148-153; | PS00008 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 29-32; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 43-46; 71-74; 121-124; 179-182; 187-190; 195-198; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 27-29; 106-108; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 157-165; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 158-172; | PS00003 | |
| Hs1vu complex proteolytic subunit-like [Q4Q116] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1ofiN | |
| Percent Identity | 53% | |
| Target Region | 23-194 | |
| Template Region | 1-172 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| THR | 1 | Sidechain, Backbone amide |
| CYS | 33 | Sidechain (LYS 33 In Template) |
| GLY | 48 | Backbone amide |
| SER | 125 | Sidechain, Backbone amide |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 58-64-0 | ADENOSINE-5'-DIPHOSPHATE | 427.201 | C10 H15 N5 O10 P2 | O=P(O)(O)OP(=O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O | 1ofi |
| 4-IODO-3-NITROPHENYL ACETYL-LEUCINYL-LEUCINYL- LEUCINYL-VINYLSULFONE | 722.632 | C28 H43 I N4 O8 S | O=[N+]([O-])c1cc(cc(I)c1O)CC(=O)NC(C(=O)NC(C(=O)NC(\C=C/S(=O)(=O)C)CC(C)C)CC(C)C)CC(C)C | 1ofi | |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 1ofi |
| 14265-44-2 | PHOSPHATE ION | 94.971 | O4 P | [O-]P([O-])([O-])=O | 1ofi |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1825648/Q4Q116.pdb 2.0 172 = residues | | = | +| Ramachandran plot: 87.8% core 9.0% allow 3.2% gener 0.0% = disall | | = | *| All Ramachandrans: 10 labelled residues (out of 170) = | +| Chi1-chi2 plots: 2 labelled residues (out of 102) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 17.4 Bad contacts: = 4 | *| Bond len/angle: 5.1 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: -0.12 Covalent: -0.14 Overall: = -0.12 | | = | | M/c bond lengths: 99.8% within limits 0.2% highlighted = | | M/c bond angles: 94.1% within limits 5.9% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |