| Hypothetical protein pkac1 [Q27687] | |
|---|---|
| Systematic Name | LmjF.18.1080 [Leishmania major] |
| Gene Name | PKAC1 |
| Molecular Weight | 38212 Da |
| Protein Sequence Size | 332 |
| Function | |
| Charge | 7.5 |
| Isoelectric Point | 8.5313 pH |
| Description | Hypothetical protein pkac1 (EC 2.7.1.37) (Protein kinase A catalytic subunit). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 2.7.1.37 |
| Sequence | >tr|Q27687|Q27687_LEIMA Hypothetical protein pkac1 (EC 2.7.1.37) (Protein kinase A catalytic subunit) - Leishmania major ITKAEASQWRLSDLEMRETVGTGTFGRVRLVKHKGTGQYAALKILKKQEILRMKQVDHVM AEASLLQEIDHPFIVSMLRGYMDKNRLYILLEYVVGGELFSHLRKAGKFPNDVSKFYCAE VILAFDYLHNKTIVYRDLKPENILLDQDGNIKITDFGFAKRVTERTFTLCGTPEYLAPEI IQSKGHNKAVDWWALGILLYEMLVGYPPFFDDSPMKIYEKILVGKVLFPRWVDSKARDFI KGLLSLDPTKRLGNLPNGTEDIKNHKYFAEVDWNVVLSKKIPAPIPVRQHKEGDTHYFDK YPDSPLNPLRTLTPAQQDCFANFCNGQYTDE |
| DNA Sequence | >LmjF18.1080 |PKAC1||protein kinase A catalytic subunit|Leishmania major|chr 18|||Manual ATGATCACCA AGGCCGAAGC TTCCCAATGG AGGCTGAGCG ACTTGGAGAT GCGTGAGACGGTCGGCACCG GCACTTTCGG CCGAGTACGC TTGGTGAAGC ACAAAGGAAC AGGCCAGTACGCGGCCCTCA AGATCCTGAA GAAGCAGGAG ATTCTCCGAA TGAAGCAGGT CGACCACGTGATGGCGGAGG CAAGTCTTCT CCAGGAGATC GACCACCCTT TTATTGTGAG CATGCTGCGGGGATACATGG ACAAGAACCG GCTGTACATT CTCCTCGAGT ACGTGGTAGG CGGCGAGCTGTTTTCGCATC TCCGCAAGGC CGGCAAGTTC CCTAATGATG TCTCGAAGTT CTACTGCGCCGAGGTGATCC TAGCATTTGA CTACCTGCAC AACAAGACGA TCGTTTACCG TGATCTGAAGCCGGAGAACA TTCTCCTCGA CCAGGACGGC AACATAAAGA TCACGGATTT CGGATTCGCGAAGCGCGTGA CGGAGCGTAC GTTTACGCTT TGTGGCACAC CCGAGTACCT AGCACCCGAGATCATTCAGA GCAAGGGGCA CAACAAGGCT GTCGACTGGT GGGCGCTCGG CATCCTGCTGTACGAAATGC TGGTTGGCTA TCCGCCGTTC TTCGACGACA GCCCAATGAA GATCTACGAGAAGATCCTTG TGGGCAAGGT GCTGTTCCCA CGATGGGTAG ACTCGAAGGC CCGAGACTTCATCAAGGGCC TTCTATCCCT CGATCCCACG AAGCGCCTCG GCAATTTGCC CAACGGCACGGAGGACATCA AGAACCACAA GTACTTTGCG GAAGTGGACT GGAATGTAGT GCTGTCCAAGAAGATTCCCG CGCCAATCCC AGTGCGGCAA CACAAGGAGG GCGATACCCA CTACTTCGACAAGTACCCCG ACAGCCCACT CAACCCTTTG CGCACTTTGA CTCCAGCACA GCAGGACTGCTTCGCCAACT TCTGCAACGG CCAGTATACG GACGAGTAG |
| Hypothetical protein pkac1 Q27687] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; protein kinase activity; protein serine/threonine kinase activity; protein-tyrosine kinase activity |
| Biochemical Pathway | protein amino acid phosphorylation |
| Regulatory Pathway | |
| KEGG Pathways | K04345 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | protein kinase, X-linked [Homo sapiens] | 52 | 1e-100 | 361 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YHR205w strong similarity to S.pombe sck1 protein kinase | Saccharomyces cerevisiae | 42% | 7e-71 | 261 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 131-134; 258-261; | PS00722 | |
| PDOC00100 | Protein kinases signatures and profile | 21-44; | PS00107 | |
| PDOC00100 | Protein kinases signatures and profile | 134-146; | PS00108 | |
| PDOC00100 | Protein kinases signatures and profile | 50.52 | 15-269 | PS50011 |
| Acylation | N-myristoylation site | 38-43; 254-259; | PS00008 | |
| Glycosylation | N-glycosylation site | 131-134; 258-261; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 161-164; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 3-6; 13-16; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 164-166; 250-252; 279-281; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 292-298; | PS00007 | |
| Hypothetical protein pkac1 [Q27687] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1ctpE | |
| Percent Identity | 54% | |
| Target Region | 1-332 | |
| Template Region | 7-333 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 138 | Sidechain |
| LYS | 140 | Sidechain |
| ASN | 143 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 544-63-8 | MYRISTIC ACID | 228.371 | C14 H28 O2 | O=C(O)CCCCCCCCCCCCC | 1ctp |
| PHOSPHITE ION | 78.972 | O3 P | [O-][PH2-]([O-])=O | 1ctp | |
| 3,5-DIIODOTYROSINE | 432.982 | C9 H9 I2 N O3 | Ic1cc(cc(I)c1O)CC(C(=O)O)N | 1ctp | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1150607/Q27687.pdb 2.0 332 = residues | | = | *| Ramachandran plot: 89.0% core 9.3% allow 1.4% gener 0.3% = disall | | = | *| All Ramachandrans: 11 labelled residues (out of 330) = | +| Chi1-chi2 plots: 2 labelled residues (out of 221) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 9 | *| Bond len/angle: 6.9 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.00 Covalent: -0.22 Overall: = -0.08 | | = | | M/c bond lengths: 98.5% within limits 1.5% highlighted = | | M/c bond angles: 93.1% within limits 6.9% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |