| Pteridine reductase 1 [Q01782] | |
|---|---|
| Systematic Name | LmjF.23.0270 [Leishmania major] |
| Gene Name | PTR1,HMTXR |
| Molecular Weight | 30457 Da |
| Protein Sequence Size | 288 |
| Function | Exhibits a NADPH-dependent biopterin reductase activity. Has good activity with folate and significant activity with dihydrofolate and dihydrobiopterin, but not with quinonoiddihydrobiopterin. Confers resistance to methotrexate (MTX). |
| Charge | 3 |
| Isoelectric Point | 7.188 pH |
| Description | Pteridine reductase 1 (EC 1.5.1.33) (H region methotrexate resistance |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 1.5.1.33 |
| Sequence | >sp|Q01782|PTR1_LEIMA Pteridine reductase 1 (EC 1.5.1.33) (H region methotrexate resistance protein) - Leishmania major TAPTVPVALVTGAAKRLGRSIAEGLHAEGYAVCLHYHRSAAEANALSATLNARRPNSAIT VQADLSNVATAPVSGADGSAPVTLFTRCAELVAACYTHWGRCDVLVNNASSFYPTPLLRN DEDGHEPCVGDREAMETATADLFGSNAIAPYFLIKAFAHRFAGTPAKHRGTNYSIINMVD AMTNQPLLGYTIYTMAKGALEGLTRSAALELAPLQIRVNGVGPGLSVLVDDMPPAVWEGH RSKVPLYQRDSSAAEVSDVVIFLCSSKAKYITGTCVKVDGGYSLTRA |
| DNA Sequence | >LmjF23.0270 |PTR1||pteridine reductase 1|Leishmania major|chr 23|||Manual ATGACTGCTC CGACCGTGCC GGTGGCGTTG GTAACAGGCG CCGCGAAGCG TCTTGGCCGCAGTATCGCTG AGGGACTCCA CGCGGAGGGG TACGCTGTCT GCTTGCACTA TCATCGCTCTGCTGCAGAAG CGAACGCACT ATCCGCGACG CTCAACGCAA GGCGACCGAA CAGCGCCATCACGGTGCAGG CGGATCTGAG CAACGTTGCC ACAGCCCCGG TCAGCGGCGC TGATGGCTCTGCACCTGTTA CCCTCTTCAC GCGCTGTGCT GAGTTGGTGG CTGCGTGCTA CACCCACTGGGGACGCTGCG ACGTGCTAGT GAACAACGCC TCTTCTTTCT ACCCCACGCC GCTGCTGAGGAATGACGAGG ATGGACACGA GCCCTGTGTC GGAGATAGAG AGGCAATGGA GACGGCCACCGCTGACCTCT TCGGCTCCAA CGCGATAGCG CCCTACTTCT TGATTAAGGC GTTCGCGCATCGCGTCGCGG GCACCCCAGC CAAGCATCGC GGCACCAACT ACTCCATCAT CAACATGGTCGACGCCATGA CGAACCAGCC TCTTCTCGGG TACACCATAT ATACCATGGC CAAAGGGGCGTTGGAGGGGC TGACACGGTC TGCCGCGCTG GAGCTTGCGC CGCTGCAGAT TCGAGTGAACGGCGTTGGTC CGGGTTTGTC GGTGCTCGTC GATGACATGC CCCCTGCTGT GTGGGAGGGCCACCGCAGCA AGGTGCCTCT GTACCAGCGC GATTCCTCCG CCGCAGAGGT GAGCGACGTTGTTATCTTTC TGTGCTCCTC CAAGGCCAAG TACATCACCG GCACCTGTGT CAAAGTGGATGGTGGCTACA GCCTTACCCG GGCCTGA |
| Pteridine reductase 1 Q01782] | |
|---|---|
| Metabolite Information | metabolism |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K03331 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | dicarbonyl/L-xylulose reductase [Homo sapiens] | 27 | 0.000000000000008 | 78 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| fabG 3-Oxoacyl-[acyl-carrier protein] reductase | Staphylococcus aureus | 31% | 2e-20 | 94 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 109-112; 173-176; | PS00722 | |
| PDOC00299 | Microbodies C-terminal targeting signal | 285-287; | PS00342 | |
| PDOC00060 | Short-chain dehydrogenases/reductases family signature | 181-209; | PS00061 | |
| Acylation | N-myristoylation site | 76-81; 171-176; 199-204; 203-208; 221-226; 281-286; | PS00008 | |
| Glycosylation | N-glycosylation site | 109-112; 173-176; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 21-24; 40-43; 75-78; 253-256; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 266-268; 284-286; | PS00005 | |
| Pteridine reductase 1 [Q01782] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2bfpD | |
| Percent Identity | 99% | |
| Target Region | 1-288 | |
| Template Region | 5-257 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| THR | 165 | Sidechain |
| TYR | 194 | Sidechain |
| LYS | 198 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 25322-68-3 | 1,2-ETHANEDIOL | 62.068 | C2 H6 O2 | OCCO | 2bfp |
| 5,6,7,8-TETRAHYDROBIOPTERIN | 241.247 | C9 H15 N5 O3 | O=C1C=2NC(CNC=2N=C(N1)N)C(O)C(O)C | 2bfp | |
| NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | 743.405 | C21 H28 N7 O17 P3 | c1cc(c[n+](c1)C2C(C(C(O2)COP(=O)([O-])OP(=O)(O)OCC3C(C(C(O3)n4cnc5c4ncnc5N)OP(=O)(O)O)O)O)O)C(=O)N | 2bfp | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1563016/Q01782.pdb 2.0 288 = residues | | = | +| Ramachandran plot: 92.7% core 6.9% allow 0.4% gener 0.0% = disall | | = | +| All Ramachandrans: 6 labelled residues (out of 286) = | +| Chi1-chi2 plots: 2 labelled residues (out of 135) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 10.9 Bad contacts: = 4 | *| Bond len/angle: 5.6 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.13 Covalent: -0.13 Overall: = 0.03 | | = | | M/c bond lengths: 98.8% within limits 1.2% highlighted = | | M/c bond angles: 95.3% within limits 4.7% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |