| Cdc2-related kinase [O96526] | |
|---|---|
| Systematic Name | LmjF.36.0550 [Leishmania major] |
| Gene Name | CRK3 |
| Molecular Weight | 35644 Da |
| Protein Sequence Size | 311 |
| Function | |
| Charge | 3.5 |
| Isoelectric Point | 7.2997 pH |
| Description | Cdc2-related kinase (Cell division related protein kinase 2). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|O96526|O96526_LEIMA Cdc2-related kinase (Cell division related protein kinase 2) - Leishmania major. SSFGRVTARSGDAGTRDSLDRYNRLDVLGEGTYGVVYRAVDKITGQYVALKKVRLDRTEE GIPQTALREVSILQEFDHPNIVNLLDVICSDGKLYLVFEYVEADLKKAIEKQEGGYSGMD LKRLIYQLLDGLYFCHRHRIIHRDLKPANILLTSGNVLKLADFGLARAFQVPMHTYTHEV VTLWYRAPEILLGEKHYTPAVDMWSVGCIFAELTRRKVLFRGDSEIGQLFEIFQVLGTPT DTEGSWPGVSRLPDYRDVFPKWTAKRLGQVLPELHPDAIDLLSKMLKYDPRERISAKEAL QHPWFSDLRW |
| DNA Sequence | >LmjF36.0550 |CRK3||cell division related protein kinase 2|Leishmania major|chr 36|||Manual ATGTCTTCGT TTGGCCGTGT TACCGCCCGT AGCGGTGACG CTGGGACGCG TGACAGTCTTGACCGGTACA ATCGCTTGGA TGTTTTGGGA GAGGGAACGT ATGGTGTTGT GTATCGCGCGGTCGACAAAA TCACTGGGCA GTACGTTGCT CTCAAGAAAG TGCGACTCGA TCGCACTGAGGAGGGCATTC CGCAGACTGC GCTGCGCGAG GTGTCGATTC TACAAGAGTT CGACCACCCCAACATTGTGA ACTTGCTTGA TGTCATTTGC TCGGACGGGA AGCTCTACCT TGTCTTCGAGTATGTGGAGG CGGACCTGAA AAAGGCGATT GAAAAGCAAG AGGGTGGATA CTCTGGAATGGATCTGAAGC GGCTTATTTA TCAGCTTTTA GACGGCCTTT ACTTTTGCCA CCGCCATCGCATCATCCACC GTGATCTGAA GCCAGCCAAC ATCCTCCTGA CATCAGGGAA CGTCCTTAAATTGGCTGATT TCGGTCTCGC CCGTGCGTTC CAAGTGCCCA TGCACACCTA CACGCACGAGGTGGTTACAC TGTGGTACCG TGCCCCTGAG ATCCTCCTCG GTGAGAAGCA CTACACTCCTGCCGTGGATA TGTGGAGTGT CGGCTGTATT TTCGCTGAGC TAACGCGCCG AAAGGTTCTTTTCCGCGGCG ATAGCGAAAT CGGGCAGTTG TTCGAGATTT TTCAAGTGCT GGGGACGCCGACGGACACCG AGGGGTCCTG GCCTGGTGTG TCGCGGCTGC CCGACTACCG CGACGTATTTCCCAAGTGGA CTGCAAAGCG CCTGGGGCAG GTACTACCAG AACTTCATCC GGACGCTATTGATCTGCTCT CCAAGATGCT CAAGTACGAT CCGCGGGAGC GCATATCAGC CAAGGAGGCCCTTCAGCACC CGTGGTTCAG TGACCTTCGC TGGTAG |
| Cdc2-related kinase O96526] | |
|---|---|
| Metabolite Information | cell cycle; protein amino acid phosphorylation |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K02087 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | cyclin-dependent kinase 3 [Homo sapiens] | 59 | 1e-101 | 364 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YBR160w cyclin-dependent protein kinase | Saccharomyces cerevisiae | 54% | 3e-89 | 322 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00016 | Cell attachment sequence | 222-224 | PS00016 | |
| PDOC00100 | Protein kinases signatures and profile | 29-52 | PS00107 | |
| PDOC00100 | Protein kinases signatures and profile | 141-153 | PS00108 | |
| PDOC00100 | Protein kinases signatures and profile | 23-306 | PS50011 | |
| Acylation | N-myristoylation site | 15-20; 46-51; 62-67; 115-120; 132-137; 165-170; 208-213; 245-250 | PS00008 | |
| Phosphorylation | Casein kinase II phosphorylation site | 118-121; 239-242; 241-244; 296-299 | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 8-10; 215-217; 264-266; 296-298 | PS00005 | |
| Cdc2-related kinase [O96526] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2iw8C | |
| Percent Identity | 58% | |
| Target Region | 19-132 | |
| Template Region | 1-265 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 127 | Sidechain |
| LYS | 129 | Sidechain |
| ASN | 132 | Sidechain |
| THR | 165 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| O6-CYCLOHEXYLMETHOXY-2-(4'-SULPHAMOYLANILINO) PURINE | 402.471 | C18 H22 N6 O3 S | O=S(=O)(N)c1ccc(cc1)Nc3nc2c(ncn2)c(n3)OCC4CCCCC4 | 2iw8 | |
| 96-27-5 | MONOTHIOGLYCEROL | 108.159 | C3 H8 O2 S | OCC(O)CS | 2iw8 |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
|
|
|
| Modeled Protein | Template Structure |
|
|
+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/871640/O96526.pdb 2.0 293 = residues | | = | *| Ramachandran plot: 90.7% core 8.6% allow 0.4% gener 0.4% = disall | | = | +| All Ramachandrans: 7 labelled residues (out of 291) = | +| Chi1-chi2 plots: 2 labelled residues (out of 192) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 5.5 Bad contacts: = 3 | *| Bond len/angle: 16.6 Morris et al class: 1 = 1 2 | +| 2 cis-peptides = | | G-factors Dihedrals: 0.03 Covalent: -0.25 Overall: = -0.07 | | = | | M/c bond lengths: 98.7% within limits 1.3% highlighted = | *| M/c bond angles: 93.5% within limits 6.5% highlighted 2 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |