| AP-Endonuclease [O15826] | |
|---|---|
| Systematic Name | LmjF.06.0560 [Leishmania major] |
| Gene Name | DUT |
| Molecular Weight | 30354 Da |
| Protein Sequence Size | 268 |
| Function | |
| Charge | -6 |
| Isoelectric Point | 4.8783 pH |
| Description | DUTPase (EC 3.6.1.23) (Deoxyuridine triphosphatase, putative (Dutp diphosphatase) (EC 3.6.1.23)). |
| Subcellular Location | Mitochondrion[Predict] |
| E. C. Number | 3.6.1.23 |
| Sequence | >tr|O15826|O15826_LEIMA DUTPase (EC 3.6.1.23) (Deoxyuridine triphosphatase, putative (Dutp diphosphatase) (EC 3.6.1.23)) - Leishmania major. KRARSANIPGAILHSLAELQDGLNAMIDPSWRAVRSLDNWALAITMESTELLDSYPWKWW KNLNATPDLANVRIELVDIFHFSLSGAMQMRSTPDDEIPAASLKPLKEVMTTFLPAKECT SDPYGFVFFPLTDTQNAIASFRNIIQLANAYRFDVIIECIIYAAEDLGFNLVAYYIAKHT LNCIRQLSGYKDGSYVKVNNGVEDNSLLHNCIKDVSLDEVLDADKYVQAWNSIMANVYEA FQIKESDRKDAERWFALAKENRLAIKA |
| DNA Sequence | >LmjF06.0560 |DUT||deoxyuridine triphosphatase, putative|Leishmania major|chr 6|||Manual ATGAAGCGCG CTCGCAGCGC CAATATTCCA GGTGCGATTC TGCACTCCTT GGCAGAGCTGCAGGATGGCC TGAACGCGAT GATCGACCCT TCCTGGCGCG CTGTTCGCTC GCTGGACAATTGGGCTCTAG CCATCACGAT GGAGTCGACG GAGCTGCTGG ACTCCTATCC GTGGAAGTGGTGGAAGAACC TCAACGCTAC GCCTGATTTG GCCAACGTTA GGATTGAACT GGTCGACATCTTTCACTTTT CCCTTTCGGG CGCTATGCAA ATGCGGTCTA CCCCGGACGA TGAGATTCCCGCCGCTTCGC TCAAGCCTCT GAAGGAGGTC ATGACAACTT TTCTGCCGGC TAAGGAGTGCACGAGCGACC CTTACGGCTT TGTGTTCTTT CCCCTCACCG ATACACAGAA CGCCATTGCGAGCTTTCGTA ACATCATTCA GCTCGCAAAC GCCTATCGCT TCGATGTCAT CATAGAGTGCATCATATACG CCGCTGAGGA CCTGGGGTTT AACTTGGTGG CGTACTATAT TGCAAAACACACTCTGAACT GCATTCGCCA GCTGAGTGGC TACAAAGACG GCTCTTACGT GAAGGTGAACAATGGTGTTG AGGACAATTC ACTTCTGCAC AACTGCATCA AAGACGTATC CCTCGATGAAGTTCTCGACG CTGACAAGTA TGTCCAGGCG TGGAACAGCA TCATGGCTAA CGTTTACGAAGCTTTCCAGA TTAAGGAGTC TGACCGAAAG GATGCGGAGC GCTGGTTTGC CCTCGCAAAGGAAAATCGGC TGGCGATCAA GGCATAA |
| AP-Endonuclease O15826] | |
|---|---|
| Metabolite Information | |
| Molecular Function | dUTP diphosphatase activity |
| Biochemical Pathway | dUTP metabolism |
| Regulatory Pathway | |
| KEGG Pathways | lma00240 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | PREDICTED: hypothetical protein LOC9720 isoform 5 [Homo sapiens] | 36 | 4.8 | 29 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| HI0829 soluble lytic murein transglycosylase | Haemophilus influenzae | 32% | 1.2 | 27.7 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| Acylation | N-myristoylation site | 202-207 | PS00008 | |
| Phosphorylation | Casein kinase II phosphorylation site | 16-19; 93-96; 94-97; 217-220 | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 31-33; 103-105; 141-143; 247-249 | PS00005 | |
| AP-Endonuclease [O15826] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2cjeA | |
| Percent Identity | 100% | |
| Target Region | 1-268 | |
| Template Region | 8-258 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-DIPHOSPHATE | 387.177 | C9 H15 N3 O10 P2 | O=P(O)(O)NP(=O)(O)OCC2OC(N1C(=O)NC(=O)C=C1)CC2O | 2cje | |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 2cje |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1960686/O15826.pdb 2.0 268 = residues | | = | | Ramachandran plot: 94.4% core 5.6% allow 0.0% gener 0.0% = disall | | = | +| All Ramachandrans: 3 labelled residues (out of 266) = | +| Chi1-chi2 plots: 1 labelled residues (out of 173) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 3.9 Bad contacts: = 3 | *| Bond len/angle: 5.6 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.25 Covalent: -0.07 Overall: = 0.13 | | = | | M/c bond lengths: 99.3% within limits 0.7% highlighted = | | M/c bond angles: 96.0% within limits 4.0% highlighted = | +| Planar groups: 99.0% within limits 1.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |