| Peptidyl-prolyl cis-trans isomerase [O02614] | |
|---|---|
| Systematic Name | LmjF.25.0910 [Leishmania major] |
| Gene Name | CYPA |
| Molecular Weight | 18823 Da |
| Protein Sequence Size | 177 |
| Function | |
| Charge | 3 |
| Isoelectric Point | 7.9608 pH |
| Description | Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8). |
| Subcellular Location | cytosol; flagellum[Predict] |
| E. C. Number | 5.2.1.8 |
| Sequence | >tr|O02614|O02614_LEIMA Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) - Leishmania major. PYTPHYPVVESNPKVWMDIDIGGKPAGRVTMELFKDAVPQTAENFRALCTGEKGFGYANS PFHRVIPDFMCQGGDFTNGNGTGGKSIYGSKFADESFLGKAGKHFGPGTLSMANAGPNTN GSQFFLCTAPTSWLDGKHVVFGQVLEGYEVVKAMEAVGSRSGTTSKPVRVSACGQL |
| DNA Sequence | >LmjF25.0910 |CYPA||cyclophilin a|Leishmania major|chr 25|||Manual ATGCCTTACA CGCCGCACTA CCCTGTCGTT GAATCCAACC CCAAGGTTTG GATGGATATCGACATCGGTG GCAAGCCCGC CGGCCGCGTA ACGATGGAGC TCTTCAAGGA CGCCGTTCCCCAGACGGCCG AGAACTTCCG CGCGCTGTGC ACGGGCGAGA AGGGCTTCGG CTACGCCAACTCCCCGTTCC ACCGTGTGAT CCCGGATTTC ATGTGCCAGG GTGGTGACTT CACCAACGGCAACGGCACTG GCGGCAAGTC CATCTACGGC TCCAAGTTTG CCGATGAGTC CTTTCTCGGCAAGGCCGGCA AGCACTTCGG CCCAGGCACG CTGTCGATGG CCAATGCCGG CCCCAACACGAACGGCTCTC AGTTCTTCCT GTGCACAGCG CCCACGAGCT GGCTGGACGG CAAGCACGTCGTGTTCGGCC AGGTGCTGGA GGGCTATGAA GTGGTCAAGG CTATGGAGGC CGTCGGCAGCCGCAGCGGCA CCACCTCGAA GCCCGTGCGC GTGTCTGCCT GCGGGCAGCT TTAA |
| Peptidyl-prolyl cis-trans isomerase O02614] | |
|---|---|
| Metabolite Information | |
| Molecular Function | peptidyl-prolyl cis-trans isomerase activity |
| Biochemical Pathway | protein folding |
| Regulatory Pathway | |
| KEGG Pathways | K09565 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | peptidylprolyl isomerase F precursor [Homo sapiens] | 64 | 7e-58 | 219 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| ppiB Peptidyl-prolyl cis-trans isomerase B (EC 5.2.1.8) | Escherichia coli MG1655 | 29% | 0.000001 | 46.6 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00017 | ATP/GTP-binding site motif A (P-loop) | 80-87 | PS00017 | |
| PDOC00154 | Cyclophilin-type peptidyl-prolyl cis-trans isomerase signature & profile | 58-75 | PS00170 | |
| PDOC00154 | Cyclophilin-type peptidyl-prolyl cis-trans isomerase signature & profile | 43.551 | 17-176 | PS50072 |
| Acylation | N-myristoylation site | 23-28; 74-79; 80-85; 90-95; 159-164 | PS00008 | |
| Glycosylation | N-glycosylation site | 81-84; 121-124 | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 133-136 | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 165-167 | PS00005 | |
| Peptidyl-prolyl cis-trans isomerase [O02614] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2biuX | |
| Percent Identity | 64% | |
| Target Region | 12-177 | |
| Template Region | 2-164 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ARG | 54 | Sidechain |
| PHE | 59 | Sidechain |
| GLN | 62 | Sidechain |
| ASN | 104 | Sidechain |
| PHE | 115 | Sidechain |
| LEU | 124 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 8070-53-9 | DIMETHYL SULFOXIDE | 78.133 | C2 H6 O S | CS(=O)C | 2biu |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/4422384/O02614.pdb 2.0 166 = residues | | = | +| Ramachandran plot: 86.9% core 12.3% allow 0.8% gener 0.0% = disall | | = | *| All Ramachandrans: 8 labelled residues (out of 164) = | | Chi1-chi2 plots: 0 labelled residues (out of 80) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.1 Bad contacts: = 0 | *| Bond len/angle: 9.1 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: -0.04 Covalent: -0.22 Overall: = -0.11 | | = | | M/c bond lengths: 97.9% within limits 2.1% highlighted = | *| M/c bond angles: 91.9% within limits 8.1% highlighted 2 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |